FlyBase Allele Report: Dmel\daw[3]

General Information

Symbol

Dmel\daw³

Species

D. melanogaster

Name

FlyBase ID

FBal0211071

Feature type

allele

Associated gene

Dmel\daw

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Parker et al., 2006)

Mutagen

ethyl methanesulfonate

(Parker et al., 2006)

Progenitor genotype

Cytology

Description

Contains a 2bp deletion at 1893-1894, which results in a frame shift in the prodomain. This is predicted to result in a truncated protein at L515.

(Parker et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:2,811,279..2,811,280 [+]

Comment:

Deletion of nucleotides 1893-1894 (CA) of the cDNA leads to a frameshift at amino acid 450 and early translation termination.

(Parker et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage

(Pentek et al., 2009, Parker et al., 2006)

abnormal neuroanatomy | embryonic stage (with daw^Δ2)

(Parker et al., 2006)

decreased rate of movement | larval stage

(Parker et al., 2006)

lethal - all die before end of pupal stage

(Parker et al., 2006)

some die during pupal stage

(Parker et al., 2006)

uncoordinated | adult stage

(Parker et al., 2006)

Phenotype Manifest In

larval abdominal segmental nerve

(Parker et al., 2006)

larval abdominal segmental nerve (with daw^Δ2)

(Parker et al., 2006)

larval intersegmental nerve

(Parker et al., 2006)

larval intersegmental nerve (with daw^Δ2)

(Parker et al., 2006)

larval intersegmental nerve branch ISNb of A1-7

(Pentek et al., 2009)

Detailed Description

Statement

Reference

Homozygous embryos show stalling of ISNb axons at muscle 13 and incomplete extension with failure to form a synapse at muscle 12.

(Pentek et al., 2009)

Photoreceptor axon targeting defects are not seen in daw³/daw⁴ larvae.

(Zhu et al., 2008)

The majority (68%) of daw³ mutants die as white prepupae or as pharate adults and do not eclose. When reared at low density on nutritive agar plates, 7% eclose but most die shortly after eclosion. Rare escapers are fertile.

The CNS of stage 16/17 daw³ embryos show occasional breaks and fusions of the longitudinal axon fascicles at the low incidence of 5%. The glial cell population shows no overt defects. Motorneuron pathfinding is disrupted in these embryos as although the ISNb and SNa axons exit the ventral nerve cord correctly and extend into their target field, they fail to advance far enough to innervate the appropriate muscle. The ISNb axons commonly stall at muscle 6 and fail to form synapses on muscles 12 and 13, or reach muscle 12 but are unable to form a synapse. The SNa usually extends into the target muscle but frequently exhibits the loss of one or both branches. In embryos from daw³/daw³ mothers mated to daw^δ2/daw^δ2 fathers, the incidence of ISNb and SNa defects is higher than in zygotic nulls.

(Parker et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

daw³ has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by babo[+]/babo³²

(Parker et al., 2006)

daw³ has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by put⁸⁸/put[+]

(Parker et al., 2006)

Enhancer of

Statement

Reference

daw[+]/daw³ is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of babo³²

(Parker et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

daw³ has larval intersegmental nerve phenotype, enhanceable by babo[+]/babo³²

(Parker et al., 2006)

daw³ has larval intersegmental nerve phenotype, enhanceable by put⁸⁸/put[+]

(Parker et al., 2006)

Enhancer of

Statement

Reference

daw[+]/daw³ is an enhancer of larval intersegmental nerve phenotype of babo³²

(Parker et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

In daw³/+; babo³²/+; double mutant embryos, 20% of hemisegments show ISNb pathfinding defects as opposed to only 2% in daw³/+ single mutants and 4% in babo³²/+ single mutants.

In daw³/+; put⁸⁸/+ double mutant embryos, 14% of hemisegments show ISNb pathfinding defects as opposed to only 2% in daw³/+ single mutants and no defects in put⁸⁸/+ single mutants.

(Parker et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

daw^Δ1

(Parker et al., 2006)

Rescued by

daw^Δ1/daw³ is rescued by daw^UAS.cPa/Scer\GAL4^repo

(Parker et al., 2006)

daw^Δ1/daw³ is rescued by daw^UAS.cPa/Scer\GAL4^Mef2.PR

(Parker et al., 2006)

daw^Δ1/daw³ is rescued by daw^UAS.cPa/Scer\GAL4^RapGAP1-OK6

(Parker et al., 2006)

daw³ is rescued by daw^+t7.8

(Parker et al., 2006)

Comments

Two copies of daw^Scer\UAS.cPa, under the control of Scer\GAL4^repo or Scer\GAL4^Mef2.PR, rescues the axon guidance defects of daw³/daw^δ1 embryos, while one copy provides partial rescue. Expression driven by Scer\GAL4^OK6 also reverses the defects.

(Parker et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

daw³

(Ellis et al., 2010, Pentek et al., 2009, Zhu et al., 2008, Parker et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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