Imprecise excision of the P{GAL4}GH146 insertion.
Homozygous first and second instar larvae show characteristic gut staining after dye has been added to the food, indicating that the lethality of these animals is not due to a feeding defect.
Homozygous and Oaz93/Df(2R)L48 larvae have thinner and more elongated filzkorper than control larvae, while the spiracular hairs look normal in the mutants.
The main branches of the tracheal system are only partially filled with air in homozygous larvae, in contrast to wild type where air fills the entire tracheal system.
The spiracular chamber cells appear normal in shape and position in mutant embryos (at stages 11 to 17).