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General Information
Symbol
Dmel\P{GawB}GH146
Species
D. melanogaster
Name
FlyBase ID
FBti0016783
Also Known As
GH146-GAL4, GAL4-GH146, GH146
Description
Inserted Element
Uses

GAL4 reporter/driver

Affected gene(s)
Viability / fertility
viable
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
2R ( 51A4 )
Sequence Location
2R:14,458,431..14,458,452 [-]
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:14,458,431..14,458,452 [-]
Orientation
Cytological location (computed by FlyBase)
51A4 (inferred by FlyBase from sequence location)
Cytological location (reported)
51B--51C (in situ hybridization reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
Location-dependent role
Size (Kb)

11.279

Associated alleles
Molecular map
Affected Gene(s)
Insertion may affect gene
(Li et al., 2013, Acebes et al., 2012, Akerboom et al., 2012, Berdnik et al., 2012, Chen et al., 2012, Hong et al., 2012, Houot et al., 2012, Masuyama et al., 2012, Moraru et al., 2012, Naganos et al., 2012, Sudhakaran et al., 2012, Volders et al., 2012, Weng et al., 2012, Awasaki et al., 2011, Chen et al., 2011, Christiansen et al., 2011, Hampel et al., 2011, Li et al., 2011, López-Arias et al., 2011, Michels et al., 2011, Pitman et al., 2011, Sweeney et al., 2011, Thum et al., 2011, Wang et al., 2011, Wu et al., 2011, de Haro et al., 2010, Diao et al., 2010, Hekmat-Scafe et al., 2010, Pauls et al., 2010, Pauls et al., 2010, Potter and Luo, 2010, Ruta et al., 2010, Tamura et al., 2010, Tea et al., 2010, Yu et al., 2010, Yu et al., 2010, Blum et al., 2009, Gordon and Scott, 2009, Gu et al., 2009, Hong et al., 2009, Hong et al., 2009, Liu and Davis, 2009, Masuda-Nakagawa et al., 2009, Sakurai et al., 2009, Tian et al., 2009, Berdnik et al., 2008, Berdnik et al., 2008, Bolduc et al., 2008, Chen et al., 2008, Das et al., 2008, Lai et al., 2008, Lichtneckert et al., 2008, Schuldiner et al., 2008, Tanaka et al., 2008, Keleman et al., 2007, Komiyama and Luo, 2007, Komiyama et al., 2007, Lin et al., 2007, Rolls et al., 2007, Shang et al., 2007, Spletter et al., 2007, Zhang et al., 2007, Ashraf et al., 2006, Berdnik et al., 2006, Bhandari et al., 2006, Lai and Lee, 2006, Zhu et al., 2006, Zhu et al., 2006, Bettencourt da Cruz et al., 2005, Marin et al., 2005, Masuda-Nakagawa et al., 2005, Melcher and Pankratz, 2005, Ramaekers et al., 2005, Riemensperger et al., 2005, Stockinger et al., 2005, Wilson and Laurent, 2005, Hummel and Zipursky, 2004, Jefferis et al., 2004, Jhaveri et al., 2004, Suh et al., 2004, Wang et al., 2004, Wilson et al., 2004, Yu et al., 2004, Zhu and Luo, 2004, Ang et al., 2003, Hummel et al., 2003, Komiyama et al., 2003, Wang et al., 2003, Fiala et al., 2002, Jhaveri and Rodrigues, 2002, Ng et al., 2002, Python and Stocker, 2002, Python and Stocker, 2002, Schwaerzel et al., 2002, Wong et al., 2002, Heimbeck et al., 2001, Jefferis et al., 2001, Stocker et al., 1997)
Alleles and Phenotypes
Allele
Reference(s)
Reference(s)
(Arguello et al., 2021, Douthit et al., 2021, Golovin et al., 2021, Harrell et al., 2021, Hidalgo et al., 2021, Pooryasin et al., 2021, Vogt et al., 2021, Xie et al., 2021, Cachero et al., 2020, Donnelly et al., 2020, Grabe et al., 2020, He et al., 2020, Hing et al., 2020, Jung et al., 2020, Lee et al., 2020, Son and Choi, 2020, Bhukel et al., 2019, Bielopolski et al., 2019, Brown et al., 2019, Jantrapirom et al., 2019, Kaur et al., 2019, Nojima et al., 2019, Phan et al., 2019, Wu et al., 2019, Zhang et al., 2019, Zhao et al., 2019, Zhou et al., 2019, Astigarraga et al., 2018, Bakthavachalu et al., 2018, Bronk et al., 2018, Davie et al., 2018, Flybrain Neuron Database et al., 2018.6.28, Fulterer et al., 2018, Gouzi et al., 2018, Hussain et al., 2018, Jeanne et al., 2018, Jing et al., 2018, Louis et al., 2018, Lüdke et al., 2018, Mayseless et al., 2018, Utashiro et al., 2018, Zhao et al., 2018, Bienkowski et al., 2017, Boppana et al., 2017, Franco et al., 2017, Kremer et al., 2017, Li et al., 2017, Martelli et al., 2017, Minocha et al., 2017, Sethi and Wang, 2017, Shen et al., 2017, Tobin et al., 2017, Ueno et al., 2017, Wu et al., 2017, Wu et al., 2017, Carreira-Rosario et al., 2016, Cervantes-Sandoval et al., 2016, Drago and Davis, 2016, Gai et al., 2016, Grabe et al., 2016, Grover et al., 2016, Guven-Ozkan et al., 2016, Miyake et al., 2016, Ng et al., 2016, Rybak et al., 2016, Scholz-Kornehl and Schwärzel, 2016, Vogt et al., 2016, Fosque et al., 2015, Gao et al., 2015, Grabe et al., 2015, Hartenstein et al., 2015, Haynes et al., 2015, Liu et al., 2015, Lovick and Hartenstein, 2015, Macpherson et al., 2015, Mullin et al., 2015, Okumura et al., 2015, Pech et al., 2015, Sakuma et al., 2015, Shih et al., 2015, Andlauer et al., 2014, Fişek and Wilson, 2014, Kohl et al., 2014, Kohl et al., 2014, Lin et al., 2014, Luhur et al., 2014, Mishra-Gorur et al., 2014, Sakuma et al., 2014, Schubert et al., 2014, Sudhakaran et al., 2014, Wu et al., 2014, Bader et al., 2013, Cao et al., 2013, Chen et al., 2013, Das et al., 2013, Fujita et al., 2013, Levy and Larsen, 2013, Liang et al., 2013, Li et al., 2013, Li et al., 2013, Lin et al., 2013, Lin et al., 2013, Melnattur et al., 2013, Pech et al., 2013, Sekine et al., 2013, Singh et al., 2013, Tamura et al., 2013, Wu et al., 2013, Acebes et al., 2012, Akerboom et al., 2012, Berdnik et al., 2012, Butcher et al., 2012, Chen et al., 2012, Hong et al., 2012, Houot et al., 2012, Kao et al., 2012, Kuo et al., 2012, Lin et al., 2012, Masuyama et al., 2012, Moraru et al., 2012, Naganos et al., 2012, Sudhakaran et al., 2012, Tanaka et al., 2012, Volders et al., 2012, Weng et al., 2012, Awasaki et al., 2011, Chen et al., 2011, Christiansen et al., 2011, Das et al., 2011, Hampel et al., 2011, Li et al., 2011, López-Arias et al., 2011, McCann et al., 2011, Michels et al., 2011, Mochizuki et al., 2011, Ping and Tsunoda, 2011, Pitman et al., 2011, Sweeney et al., 2011, Thum et al., 2011, Wang et al., 2011, Wu et al., 2011, Chou et al., 2010, Das et al., 2010, de Haro et al., 2010, Diao et al., 2010, Hekmat-Scafe et al., 2010, Pauls et al., 2010, Pauls et al., 2010, Potter and Luo, 2010, Ruta et al., 2010, Shuai et al., 2010, Tamura et al., 2010, Tea et al., 2010, Yu et al., 2010, Yu et al., 2010, Avery et al., 2009, Blum et al., 2009, Gordon and Scott, 2009, Gu et al., 2009, Hong et al., 2009, Hong et al., 2009, Ignell et al., 2009, Leiss et al., 2009, Liu and Davis, 2009, Masuda-Nakagawa et al., 2009, Sakurai et al., 2009, Tian et al., 2009, Berdnik et al., 2008, Berdnik et al., 2008, Bolduc et al., 2008, Chen et al., 2008, Das et al., 2008, Hong et al., 2008, Lai et al., 2008, Lichtneckert et al., 2008, Root et al., 2008, Schuldiner et al., 2008, Tanaka et al., 2008, Chihara et al., 2007, Keleman et al., 2007, Komiyama and Luo, 2007, Komiyama et al., 2007, Lin et al., 2007, Rolls et al., 2007, Roy et al., 2007, Schwaerzel et al., 2007, Shang et al., 2007, Shi et al., 2007, Spletter et al., 2007, Yao et al., 2007, Zhang et al., 2007, Ashraf et al., 2006, Berdnik et al., 2006, Bhandari et al., 2006, Lai and Lee, 2006, Zhu et al., 2006, Zhu et al., 2006, Bettencourt da Cruz et al., 2005, Marin et al., 2005, Masuda-Nakagawa et al., 2005, Melcher and Pankratz, 2005, Ramaekers et al., 2005, Riemensperger et al., 2005, Stockinger et al., 2005, Wilson and Laurent, 2005, Hummel and Zipursky, 2004, Jefferis et al., 2004, Jhaveri et al., 2004, Suh et al., 2004, Wang et al., 2004, Wilson et al., 2004, Yu et al., 2004, Zhu and Luo, 2004, Ang et al., 2003, Hummel et al., 2003, Komiyama et al., 2003, Wang et al., 2003, Fiala et al., 2002, Jhaveri and Rodrigues, 2002, Ng et al., 2002, Python and Stocker, 2002, Python and Stocker, 2002, Schwaerzel et al., 2002, Wong et al., 2002, Heimbeck et al., 2001, Jefferis et al., 2001, Stocker et al., 1997)
Reference(s)
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Drives expression in projection neurons, particularly antennal lobe projection neurons.

Scer\GAL4GH146 is expressed in several cells of the adult brain, including a subset of antennal lobe projection neurons and in the mushroom body anterior paired lateral neurons.

Scer\GAL4GH146 labels a subset of projection neurons and the mushroom body anterior paired lateral cell. Co-expression of Scer\GAL4GH146 with a presynaptic marker preferentially labels the presynaptic terminals of the mushroom body anterior paired medial cell throughout the mushroom body with stronger expression in the alpha'/beta' lobes.

Scer\GAL4GH146 labels the antennal lobe projection neurons. Co-expression with a postsynaptic marker reveals that in the mushroom body calyx, these sites are juxtaposed to the presynapses of the Kenyon cells.

Labelling is observed in olfactory projection neurons.

Scer\GAL4GH146 is estimated to be expressed in one fifth of the lPN population.

Scer\GAL4GH146 is expressed in an average of 73, 193 and 49 cells of the adPN, lPN and vPN lineages, respectively.

Scer\GAL4GH146 is expressed in approximately 60% of projection neurons. An estimated 94% of cells expressing Scer\GAL4GH146 are cholinergic.

Although most projection neurons are not GABA-immunopositive, approximately 6 Scer\GAL4GH146 positive neurons, located ventral and slightly lateral to the antennal lobes, are GABA-positive.

Scer\GAL4GH146 is expressed by approximately 90 projection neurons that innervate approximately 40 of the roughly 50 adult antennal lobe glomeruli. Scer\GAL4GH146 is expressed in presumptive larval projection neurons that send neurites that appear to innervate the mushroom body calyx and the larval equivalent of the adult lateral horn.

Scer\GAL4GH146 is estimated to express in about two thirds of the antennal lobe projection neurons.

Use of UAS-GFP with nuclear localization signal allows visualization of cell bodies. In the adult brain, expression observed in clusters of cells (identified as projection neurons) surrounding the antennal lobe in 3 anatomically distinct groups: ~41 cells dorsal-anterior, ~34 cells lateral and ~7 cells ventral. In addition, expression observed in small number of cells that are not projection neurons: 6 cells posterior and lateral to the protocerebrum and 3 cells ventral to the subesophageal ganglion.

Use of UAS-CD8-GFP allows visualization of axons and dendrites. In the adult brain, the 'projection neurons' (relay interneurons) extend dendrites to specific glomeruli in the antennal lobe; the majority send dendrites to only one glomerulus. Projection neurons that innervate the same glomerulus exhibit similar patterns of axon arborization. The majority extend axons through the iACT and synapse both in the calyx of the mushroom body and the lateral protocerebrum; a small number extend axons through the mACT or the oACT, bypass the mushroom body, and connect to the protocerebrum.

The majority of axons from neurons in which Scer\GAL4GH146 expresses project through the inner antenno-cerebral tract and synapse in both the calyx of the mushroom body and the lateral cerebrum. It is expressed in at least 4 neurons that send axons through the middle antenno-cerebral tract and a single neuron projecting through the outer antenno-cerebral tract. These latter two tracts bypass the mushroom body and connect directly with the protocerebrum.

Scer\GAL4GH146 is expressed in some subset of adult antennal lobe projection neurons of the ventral, antero-dorsal and ventral antennal lobe neuroblast lineages. Because of this expression it specifically marks a number of adult antennal lobe glomeruli, the inner and medial antenno-cerebral tracts and parts of the mushroom body calyx and lateral horn.

Scer\GAL4GH146 is expressed in approximately 90 projection neurons.

In adult brain, expressed in lateral protocerebrum (LPR), relay interneurons, inner and median antenno-cerebral tracts (iACT, mACT), and calyx of mushroom body; also in 'descending interneurons' ventral to the LPR, with processes extending into the iACT. In pupal brain, expression first observed 24h APF.

 
Reflects expression of
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (3)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (23)
Reported As
Symbol Synonym
GH146-CadN
GH146-Gal4
(Arguello et al., 2021, Golovin et al., 2021, Pooryasin et al., 2021, Cachero et al., 2020, Donnelly et al., 2020, Grabe et al., 2020, He et al., 2020, Jung et al., 2020, Kaur et al., 2019, Nojima et al., 2019, Wu et al., 2019, Zhao et al., 2019, Zhou et al., 2019, Bakthavachalu et al., 2018, Davie et al., 2018, Gouzi et al., 2018, Hussain et al., 2018, Jeanne et al., 2018, Jing et al., 2018, Louis et al., 2018, Lüdke et al., 2018, Mayseless et al., 2018, Utashiro et al., 2018, Bienkowski et al., 2017, Boppana et al., 2017, Kremer et al., 2017, Martelli et al., 2017, Grover et al., 2016, Fosque et al., 2015, Hartenstein et al., 2015, Lovick and Hartenstein, 2015, Macpherson et al., 2015, Mullin et al., 2015, Okumura et al., 2015, Pech et al., 2015, Sakuma et al., 2015, Fişek and Wilson, 2014, Kohl et al., 2014, Kohl et al., 2014, Sakuma et al., 2014, Sudhakaran et al., 2014, Wu et al., 2014, Bader et al., 2013, Chen et al., 2013, Levy and Larsen, 2013, Lin et al., 2013, Pech et al., 2013, Sekine et al., 2013, Akerboom et al., 2012, Butcher et al., 2012, Chen et al., 2012, Kuo et al., 2012, Masuyama et al., 2012, Moraru et al., 2012, Sudhakaran et al., 2012, Weng et al., 2012, Li et al., 2011, Michels et al., 2011, Wang et al., 2011, Chou et al., 2010, Ruta et al., 2010, Tamura et al., 2010, Avery et al., 2009, Gordon and Scott, 2009, Gu et al., 2009, Hong et al., 2009, Hong et al., 2009, Ignell et al., 2009, Liu and Davis, 2009, Tian et al., 2009, Bolduc et al., 2008, Root et al., 2008, Komiyama and Luo, 2007, Komiyama et al., 2007, Rolls et al., 2007, Roy et al., 2007, Schwaerzel et al., 2007, Yao et al., 2007, Zhang et al., 2007, Wilson and Laurent, 2005, Hummel and Zipursky, 2004, Jhaveri et al., 2004, Suh et al., 2004, Wilson et al., 2004, Ang et al., 2003, Hummel et al., 2003, Wang et al., 2003)
GH146Gal4
P[GawB]GH146
P{GawB}GH146
pGAL4,w+GH146
pGal4,w+GH146
Name Synonyms
Secondary FlyBase IDs
    References (231)