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Citation
Moraru, M.M., Egger, B., Bao, D.B., Sprecher, S.G. (2012). Analysis of cell identity, morphology, apoptosis and mitotic activity in a primary neural cell culture system in Drosophila.  Neural Dev. 7(1): 14.
FlyBase ID
FBrf0218691
Publication Type
Research paper
Abstract

In Drosophila, most neurogenetic research is carried out in vivo. Mammalian research demonstrates that primary cell culture techniques provide a powerful model to address cell autonomous and non-autonomous processes outside their endogenous environment. We developed a cell culture system in Drosophila using wildtype and genetically manipulated primary neural tissue for long-term observations. We assessed the molecular identity of distinct neural cell types by immunolabeling and genetically expressed fluorescent cell markers. We monitored mitotic activity of cell cultures derived from wildtype and tumorous larval brains. Our system provides a powerful approach to unveil developmental processes in the nervous system and to complement studies in vivo.

PubMed ID
PubMed Central ID
PMC3379951 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Neural Dev.
    Title
    Neural Development
    Publication Year
    2006-
    ISBN/ISSN
    1749-8104
    Data From Reference