adPN neuroblast clones (induced in the early first instar larva and analysed in the adult brain) that are expressing emsScer\UAS.cRa under the control of Scer\GAL4GH146 show normal cell body projection and axonal projection trajectory, but show marked defects in dendritic targeting, with two types of phenotype being observed. Firstly, the mutant adPNs show ectopic innervation of one or more inappropriate glomeruli; the VA2 glomerulus is ectopically innervated in 100% of clones, VA6 in 78%, VL2 in 78%, VM1 in 11%, VM4 in 44%, DA2 in 78%, DC1 in 44%, DL2 in 89% and DL5 in 22%. Secondly, the mutant adPNs fail to innervate the DL1 glomerulus, which is normally innervated by these neurons in wild type.
The axons of adPN neuroblast clones expressing emsScer\UAS.cRa under the control of Scer\GAL4GH146 (induced in the early larva and analysed in adults) have ectopic terminal arbors between the main arborization area and the secondary arborization area in the lateral horn. Single cell DL1-innervating adPN clones expressing emsScer\UAS.cRa under the control of Scer\GAL4GH146 fail to innervate the DL glomerulus and ectopically innervate the DA2 and DM6 glomeruli and consistently form ectopic terminal branches between the main lateral terminal process and the secondary dorsal terminal process in the lateral horn.
Expression of emsScer\UAS.cRa under the control of Scer\GAL4sca-537.4 significantly rescues the embryonic brain defects and the "open-head" ectoderm defects of ems2 mutants.