FlyBase Allele Report: Dmel\Rab27[UASp.YFP]

General Information

Symbol

Dmel\Rab27^UASp.YFP

Species

D. melanogaster

Name

FlyBase ID

FBal0215431

Feature type

allele

Associated gene

Dmel\Rab27

Associated Insertion(s)

Carried in Construct

P{UASp-YFP.Rab27}

Also Known As

UAS-YFP-rab27

Key Links

Transgenic product class

wild_type

(Zhang et al., 2007)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Zhang et al., 2007)

Mutagen

in vitro construct

(Zhang et al., 2007)

Progenitor genotype

Carried in construct

P{UASp-YFP.Rab27}

Cytology

Description

UASp regulatory sequences drive expression of wild-type Rab27 that is tagged at the N-terminal end with YFP.

(Zhang et al., 2007)

Allele components

Component

Use(s)

Regulatory region(s)

UASp

binary expression system - regulatory region

Encoded product / tool

Rab27

Tagged with

YFP

yellow fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^VT014429/Rab27^UASp.YFP rescues Rab27^CRISPR.KO

(Lien et al., 2020)

Scer\GAL4^{Rab27-Gal4-KO}/Rab27^UASp.YFP rescues Rab27^Gal4-KO

(Lien et al., 2020)

Scer\GAL4^{Rab27-Gal4-KO}/Rab27^UASp.YFP rescues Rab27^Gal4-KO

(Chan et al., 2011)

Comments

Expression of Rab27^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^{Rab27-Gal4-KO} rescues the reduction in daytime sleep bout length which is seen in Rab27^Gal4-KO animals.

(Chan et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

9810

y¹ w^*; P{UASp-YFP.Rab27}01

24769

y¹ w^*; P{UASp-YFP.Rab27}14

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Rab27^{Scer\UAS.P\T.T:Avic\GFP-YFP}

Rab27^UASp.YFP

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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