FlyBase Allele Report: Dmel\Rab27[Gal4-KO]

General Information

Symbol

Dmel\Rab27^Gal4-KO

Species

D. melanogaster

Name

FlyBase ID

FBal0283188

Feature type

allele

Associated gene

Dmel\Rab27

Associated Insertion(s)

TI{GAL4}Rab27^Gal4-KO

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Chan et al., 2011)

Mutagen

ends-out gene targeting

(Chan et al., 2011)

FLPase

(Chan et al., 2011)

I-SceI

(Chan et al., 2011)

Progenitor genotype

Associated Insertion(s)

TI{GAL4}Rab27^Gal4-KO

Cytology

Description

The endogenous Rab27 open reading frame has been replaced by sequence comprising a GAL4 driver coding region and a loxP cassette which contains both Disc\RFP^3xP3.PC and the kanamycin resistance gene.

(Chan et al., 2011)

Allele components

Component

Use(s)

Inserted element

TI{GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

outer photoreceptor cell | adult stage

(Kohrs et al., 2021)

retina

(Kohrs et al., 2021)

rhabdomere of eye photoreceptor cell | adult stage

(Kohrs et al., 2021)

wing

(Kohrs et al., 2021)

Detailed Description

Statement

Reference

Mutant flies show a significant decrease in daytime sleep bout length compared to controls.

(Chan et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Rab27^Gal4-KO has long lived | semidominant phenotype, non-enhanceable by gig^UAS.cTa/Scer\GAL4^{Rab27-Gal4-KO}

(Lien et al., 2020)

Rab27^Gal4-KO has long lived | semidominant phenotype, non-enhanceable by Scer\GAL4^{Rab27-Gal4-KO}/S6k^STDETE.UAS

(Lien et al., 2020)

NOT suppressed by

Statement

Reference

Rab27^Gal4-KO has long lived | semidominant phenotype, non-suppressible by gig^UAS.cTa/Scer\GAL4^{Rab27-Gal4-KO}

(Lien et al., 2020)

Rab27^Gal4-KO has long lived | semidominant phenotype, non-suppressible by Scer\GAL4^{Rab27-Gal4-KO}/S6k^STDETE.UAS

(Lien et al., 2020)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rab27^Gal4-KO is an enhancer of dorsal tracheal branch primordium phenotype of Rab35^CRISPR

(Camelo et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Rab27^Gal4-KO is rescued by Scer\GAL4^{Rab27-Gal4-KO}/Rab27^UASp.YFP

(Lien et al., 2020)

Rab27^Gal4-KO is rescued by Scer\GAL4^{Rab27-Gal4-KO}/Rab27^UASp.YFP

(Chan et al., 2011)

Comments

Expression of Rab27^{Scer\UAS.P\T.T:Avic\GFP-YFP} under the control of Scer\GAL4^{Rab27-Gal4-KO} rescues the reduction in daytime sleep bout length which is seen in Rab27^Gal4-KO animals.

(Chan et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rab27^Gal4-KO

(Chan et al., 2011)

rab27^Gal4-KO

(Kohrs et al., 2021, Lien et al., 2020)

rab27^KO

(Lien et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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