FlyBase Allele Report: Scer\GAL4[Rab27-Gal4-KO]

General Information

Symbol

Scer\GAL4^{Rab27-Gal4-KO}

Species

S. cerevisiae

Name

FlyBase ID

FBal0283192

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

TI{GAL4}Rab27^Gal4-KO

Carried in Construct

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

ends-out gene targeting

(Chan et al., 2011)

FLPase

(Chan et al., 2011)

I-SceI

(Chan et al., 2011)

Progenitor genotype

Associated Insertion(s)

TI{GAL4}Rab27^Gal4-KO

Cytology

Description

The endogenous Rab27 open reading frame has been replaced by sequence comprising a GAL4 driver coding region and a loxP cassette which contains both Disc\RFP^3xP3.PC and the kanamycin resistance gene.

(Chan et al., 2011)

Allele components

Component

Use(s)

Inserted element

TI{GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Rab27

(Jin et al., 2012, Chan et al., 2011)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

long lived, with S6k^KQ.UAS

(Lien et al., 2020)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

gig^UAS.cTa/Scer\GAL4^{Rab27-Gal4-KO} is a non-enhancer of long lived | semidominant phenotype of Rab27^Gal4-KO

(Lien et al., 2020)

Scer\GAL4^{Rab27-Gal4-KO}/S6k^STDETE.UAS is a non-enhancer of long lived | semidominant phenotype of Rab27^Gal4-KO

(Lien et al., 2020)

NOT Suppressor of

Statement

Reference

gig^UAS.cTa/Scer\GAL4^{Rab27-Gal4-KO} is a non-suppressor of long lived | semidominant phenotype of Rab27^Gal4-KO

(Lien et al., 2020)

Scer\GAL4^{Rab27-Gal4-KO}/S6k^STDETE.UAS is a non-suppressor of long lived | semidominant phenotype of Rab27^Gal4-KO

(Lien et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^{Rab27-Gal4-KO}/Rab27^UASp.YFP rescues Rab27^Gal4-KO

(Lien et al., 2020)

Scer\GAL4^{Rab27-Gal4-KO}/Rab27^UASp.YFP rescues Rab27^Gal4-KO

(Chan et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^{Rab27-Gal4-KO}

rab27^Gal4-KO

(Lien et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close