FlyBase Allele Report: Dmel\rictor[Δ2]

General Information

Symbol

Dmel\rictor^Δ2

Species

D. melanogaster

Name

FlyBase ID

FBal0215849

Feature type

allele

Associated gene

Dmel\rictor

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Hietakangas and Cohen, 2007)

Progenitor genotype

P{EPgy2}rictor^EY08986

(Hietakangas and Cohen, 2007)

Cytology

Description

Imprecise excision of the P{EPgy2}rictor^EY08986 insertion, resulting in a deletion that removes rictor exons 2-4 and part of exon 5.

(Hietakangas and Cohen, 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Pink1^RNAi.UAS.cYa

(Wu et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous neurons (generated via a Scer\FLP1^SOP / MARCM method) show a complicated phenotype that combines features of both undergrowth and miniaturization.

(Shimono et al., 2014)

Mutant embryos have a normal somatic muscle pattern. The number of nuclei in the segmental border muscle is normal.

(Tixier et al., 2013)

Homozygotes show a dendritic tiling defect in class IV dendrite arborization (da) neurons, with 8.1 +/- 1.7% of dendritic branches crossing one another. In addition, the total number of dendrite branches is reduced to approximately 80% of wild type.

Single-cell dorsal class IV da neuron clones that are homozygous for rictor^Δ2 (in a heterozygous background) show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type.

The v'ada and vdaB dendrites often invade neighbouring dendritic fields in homozygous mutants, in contrast to what is seen in wild-type controls.

(Koike-Kumagai et al., 2009)

rictor^Δ1/rictor^Δ2 flies are normal in appearance.

rictor^Δ2 flies raised under controlled conditions on a rich diet show a modest (approximately 10%) reduction in body weight and a modest reduction in tissue growth (wing area is reduced) compared to controls.

rictor^Δ1 flies raised on nutrient-reduced food show growth that is indistinguishable from that of wild-type controls raised under the same conditions.

(Hietakangas and Cohen, 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

rictor^Δ2 has abnormal neuroanatomy | somatic clone phenotype, suppressible | partially by Scer\GAL4^109(2)80/mora^UAS.cSa

(Shimono et al., 2014)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2 has visible phenotype, suppressible by Marf^RNAi.UAS.cUa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2 has visible phenotype, suppressible by Atg1^UAS.cSa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2 has visible phenotype, suppressible | partially by Miro^KK102189, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2 has visible phenotype, suppressible by Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

rictor^Δ2 has abnormal neuroanatomy phenotype, suppressible | partially by trc^UAS.cUa/Scer\GAL4^ppk.PG

(Koike-Kumagai et al., 2009)

rictor^Δ2 has abnormal neuroanatomy phenotype, suppressible by Scer\GAL4^ppk.PG/trc^{UAS.Tag:Myr(Src64B)}

(Koike-Kumagai et al., 2009)

NOT suppressed by

Statement

Reference

rictor^Δ2 has abnormal neuroanatomy phenotype, non-suppressible by trc^{T449A.UAS.Tag:Myr(Src64B)}/Scer\GAL4^ppk.PG

(Koike-Kumagai et al., 2009)

Enhancer of

Statement

Reference

rictor^Δ2 is an enhancer of visible phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

rictor^Δ2 is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, foxo^UAS.GFP

(Hietakangas and Cohen, 2007)

Suppressor of

Statement

Reference

rictor^Δ2 is a suppressor of hyperplasia | somatic clone phenotype of Pten³

(Hietakangas and Cohen, 2007)

rictor^Δ2 is a suppressor of hyperplasia phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^nub-AC-62

(Hietakangas and Cohen, 2007)

NOT Suppressor of

Statement

Reference

rictor^Δ2/rictor^Δ1 is a non-suppressor of abnormal neuroanatomy phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

rictor^Δ2 is a non-suppressor of hyperplasia | somatic clone phenotype of Tsc1^R453X

(Hietakangas and Cohen, 2007)

Other

Statement

Reference

Tl^10b.UAS.cUa, rictor^Δ2 has decreased body size | pupal stage phenotype

(Roth et al., 2018)

Df(2R)Sema2b-C4, rictor^Δ2 has abnormal neuroanatomy | third instar larval stage phenotype

(Meltzer et al., 2016)

mTor^ΔP, rictor[+]/rictor^Δ2 has abnormal neuroanatomy | dominant phenotype

(Koike-Kumagai et al., 2009)

Sin1[+]/Sin1^e03756, rictor^Δ2 has abnormal neuroanatomy | dominant phenotype

(Koike-Kumagai et al., 2009)

rictor^Δ2, trc¹/trc[+] has abnormal neuroanatomy phenotype

(Koike-Kumagai et al., 2009)

Phenotype Manifest In

Suppressed by

Statement

Reference

rictor^Δ2 has abdominal anterior ventral multidendritic neuron vdaa phenotype, suppressible | partially by Scer\GAL4^109(2)80/mora^UAS.cSa

(Shimono et al., 2014)

rictor^Δ2 has dendritic tree phenotype, suppressible | partially by Scer\GAL4^109(2)80/mora^UAS.cSa

(Shimono et al., 2014)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2 has wing phenotype, suppressible by Marf^RNAi.UAS.cUa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2 has wing phenotype, suppressible by Atg1^UAS.cSa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2 has wing phenotype, suppressible | partially by Miro^KK102189, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2 has mitochondrion phenotype, suppressible by Marf^RNAi.UAS.cUa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2 has mitochondrion phenotype, suppressible by Atg1^UAS.cSa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2 has wing phenotype, suppressible by Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW, rictor^Δ2 has mitochondrion phenotype, suppressible by Hsap\PRKN^UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

rictor^Δ2 has larval dorsal multidendritic neuron ddaC phenotype, suppressible | partially by trc^UAS.cUa/Scer\GAL4^ppk.PG

(Koike-Kumagai et al., 2009)

rictor^Δ2 has larval dorsal multidendritic neuron ddaC phenotype, suppressible by Scer\GAL4^ppk.PG/trc^{UAS.Tag:Myr(Src64B)}

(Koike-Kumagai et al., 2009)

NOT suppressed by

Statement

Reference

rictor^Δ2 has larval dorsal multidendritic neuron ddaC phenotype, non-suppressible by trc^{T449A.UAS.Tag:Myr(Src64B)}/Scer\GAL4^ppk.PG

(Koike-Kumagai et al., 2009)

Enhancer of

Statement

Reference

rictor^Δ2 is an enhancer of wing phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

rictor^Δ2 is an enhancer of mitochondrion phenotype of Pink1^RNAi.UAS.cYa, Scer\GAL4^Mhc.PW

(Wu et al., 2013)

rictor^Δ2 is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, foxo^UAS.GFP

(Hietakangas and Cohen, 2007)

Suppressor of

Statement

Reference

rictor^Δ2/rictor^Δ1 is a suppressor of embryonic/larval neuromuscular junction phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

rictor^Δ2 is a suppressor of embryonic/larval salivary gland phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^nub-AC-62

(Hietakangas and Cohen, 2007)

rictor^Δ2 is a suppressor of eye | somatic clone phenotype of Pten³

(Hietakangas and Cohen, 2007)

NOT Suppressor of

Statement

Reference

rictor^Δ2/rictor^Δ1 is a non-suppressor of photoreceptor cell R7 phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

rictor^Δ2/rictor^Δ1 is a non-suppressor of photoreceptor cell R8 phenotype of Rheb^UAS.cPa, Scer\GAL4^elav.PLu

(Dimitroff et al., 2012)

rictor^Δ2 is a non-suppressor of eye | somatic clone phenotype of Tsc1^R453X

(Hietakangas and Cohen, 2007)

Other

Statement

Reference

Tl^10b.UAS.cUa, rictor^Δ2 has pupa phenotype

(Roth et al., 2018)

Df(2R)Sema2b-C4, rictor^Δ2 has larval multidendritic class IV neuron | third instar larval stage phenotype

(Meltzer et al., 2016)

Df(2R)Sema2b-C4, rictor^Δ2 has dendrite | third instar larval stage phenotype

(Meltzer et al., 2016)

mTor^ΔP, rictor[+]/rictor^Δ2 has dendrite phenotype

(Koike-Kumagai et al., 2009)

mTor^ΔP, rictor[+]/rictor^Δ2 has larval dorsal multidendritic neuron ddaC phenotype

(Koike-Kumagai et al., 2009)

Sin1[+]/Sin1^e03756, rictor^Δ2 has dendrite phenotype

(Koike-Kumagai et al., 2009)

Sin1[+]/Sin1^e03756, rictor^Δ2 has larval multidendritic class IV neuron phenotype

(Koike-Kumagai et al., 2009)

rictor^Δ2, trc¹/trc[+] has dendrite phenotype

(Koike-Kumagai et al., 2009)

rictor^Δ2, trc¹/trc[+] has larval multidendritic class IV neuron phenotype

(Koike-Kumagai et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

rictor^Δ2-homozygous or heterozygous pupae show a significant decrease in weight upon the expression of Tl^10b.UAS.cUa under the control of Scer\GAL4^r4; there is also apparently smaller pupae only in the rictor^Δ2-homozygous background.

(Roth et al., 2018)

The level of non-contacting dendrite crossing in class IV dendrite arborizing larval neurons is significantly increased in rictor^Δ2;Df(2R)Sema-2b-C4 double mutants compared to either rictor^Δ2/+ or Df(2R)Sema-2b-C4/+ heterozygotes.

(Meltzer et al., 2016)

Scer\GAL4^109(2)80-mediated overexpression of CHORD^Scer\UAS.cSa in rictor^Δ2 mutant neurons partially restores the features of the miniature phenotype, but not those of the undergrowth phenotype.

(Shimono et al., 2014)

The abnormal wing posture phenotype seen in flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW is enhanced by rictor^Δ2/Y.

The mitochondrial aggregation phenotype seen in the muscles of flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW is enhanced by rictor^Δ2.

The ability of rictor^Δ2 to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW is blocked by co-expression of either Marf^{miRNA.cUa.Scer\UAS} or Atg1^Scer\UAS.cSa.

The ability of rictor^Δ2 to enhance the abnormal wing posture phenotype seen in flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW is partially suppressed by co-expression of Miro^KK102189.

(Wu et al., 2013)

The axon guidance phenotypes of Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu flies are not significantly rescued in a rictor^Δ1/rictor^Δ2 background.

The Rheb^Scer\UAS.cPa, Scer\GAL4^elav.PLu synapse overgrowth phenotype is significantly rescued in a rictor^Δ1/rictor^Δ2 background.

(Dimitroff et al., 2012)

rictor^Δ2/+ ; Sin1^e03756/+ double heterozygotes have a dendritic tiling defect in class IV dendrite arborization (da) neurons, with a significantly higher number of dendritic branches crossing one another compared to wild type (neither single heterozygote shows this phenotype).

rictor^Δ2 ; Sin1^e03756 double homozygotes show dendritic tiling defects in class IV da neurons that are indistinguishable from the single homozygotes.

The dendritic branches of ddaC neurons in Tor^ΔP/+ ; rictor^Δ2/+ double heterozygous larvae show increased crossing over of each other, indicating a tiling defect.

Class IV da neurons of rictor^Δ2/+ ; trc¹/+ double heterozygous larvae show defects in dendritic tiling, with a significantly higher number of dendritic branches crossing one another compared to wild type.

Expression of trc^Scer\UAS.cUa under the control of Scer\GAL4^ppk.PG slightly reduces the dendritic tiling defects seen in ddaC neurons in rictor^Δ2 animals.

Expression of trc^{Scer\UAS.T:Myr1} under the control of Scer\GAL4^ppk.PG significantly rescues the dendritic tiling defects seen in ddaC neurons in rictor^Δ2 animals.

Expression of trc^{T449A.Scer\UAS.T:Myr1} under the control of Scer\GAL4^ppk.PG does not rescue the dendritic tiling defects seen in ddaC neurons in rictor^Δ2 animals.

(Koike-Kumagai et al., 2009)

The severity of the rough eye phenotype caused by expression of foxo^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PF is enhanced by rictor^Δ2 at both 18 and 25^oC.

The overgrowth of the salivary gland that is seen larvae expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^nub-AC-62 is suppressed by rictor^Δ2.

rictor^Δ2 suppresses the overgrowth of the eye caused by homozygous Pten³ clones.

rictor^Δ2 does not suppress the overgrowth of the eye caused by homozygous Tsc1^R453X clones.

(Hietakangas and Cohen, 2007)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\PARK2^Scer\UAS.cYa completely blocks the ability of rictor^Δ2 to enhance the abnormal wing posture and mitochondrial aggregation phenotypes seen in flies expressing Pink1^{dsRNA.Scer\UAS} under the control of Scer\GAL4^Mhc.PW.

(Wu et al., 2013)

Complementation and Rescue Data

Rescued by

rictor^Δ2 is rescued by rictor^UAS.cHa/Scer\GAL4^unspecified

(Koike-Kumagai et al., 2009)

rictor^Δ2 is rescued by rictor^UAS.cHa/Scer\GAL4^ppk.PG

(Koike-Kumagai et al., 2009)

rictor^Δ2 is rescued by rictor^UAS.cHa/Scer\GAL4^Tub.PU

(Hietakangas and Cohen, 2007)

Comments

Class IV neuron-specific expression of rictor^Scer\UAS.cHa largely rescues the dendritic phenotypes of single-cell rictor^Δ2 class IV dendrite arborization neuron clones.

Expression of rictor^Scer\UAS.cHa under the control of Scer\GAL4^ppk.PG largely rescues the tiling defects seen in v'ada and vdaB neurons of rictor^Δ2 mutants.

(Koike-Kumagai et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

rictor D2

(Hietakangas and Cohen, 2007)

rictor²

(Shimono et al., 2014)

rictor^Δ2

(Luo et al., 2020, Roth et al., 2018, Meltzer et al., 2016, Shimono et al., 2014, Tixier et al., 2013, Dimitroff et al., 2012, Koike-Kumagai et al., 2009, Hietakangas and Cohen, 2007)

rictorΔ2

(Wu et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (9)

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