FlyBase Allele Report: Dmel\mip120[67]

General Information

Symbol

Dmel\mip120⁶⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0216297

Feature type

allele

Associated gene

Dmel\mip120

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

P{SUPor-P}mip120^KG05422

(Beall et al., 2007)

Caused by aberration

Df(2R)mip120⁶⁷

Cytology

Description

Imprecise excision of the P{SUPor-P}mip120^KG05422 insertion resulting in a deletion which removes most of the sequences encoding mip120. Sequences encoding the EfTuM gene (which is within an intron of mip120) are completely deleted.

(Beall et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage (with Df(2R)BSC274), with mEFTu1^+tBa

(Cheng et al., 2017)

female sterile (with Df(2R)BSC274), with mEFTu1^+tBa

(Cheng et al., 2017)

female sterile | recessive

(Beall et al., 2007)

male sterile | recessive

(Beall et al., 2007)

partially lethal

(Beall et al., 2007)

visible

(Beall et al., 2007)

Phenotype Manifest In

chromosome | adult stage | female (with Df(2R)BSC274), with mEFTu1^+tBa

(Cheng et al., 2017)

egg chamber

(Beall et al., 2007)

egg chamber (with Df(2R)BSC274), with mEFTu1^+tBa

(Cheng et al., 2017)

eye

(Beall et al., 2007)

nucleus & follicle cell

(Beall et al., 2007)

nucleus & nurse cell

(Beall et al., 2007)

nurse cell (with Df(2R)BSC274), with mEFTu1^+tBa

(Cheng et al., 2017)

ovary (with Df(2R)BSC274), with mEFTu1^+tBa

(Cheng et al., 2017)

spermatozoon

(Beall et al., 2007)

Detailed Description

Statement

Reference

mip120⁶⁷ homozygotes do not show any obvious egg chambers phenotype.

(Coux et al., 2018)

mip120⁶⁷,mEFTu1^+tBa/Df(2R)BSC274 mutant flies (carrying mip120⁶⁷ recombined with mEFTu1^+tBa to rescue the mEFTu1 gene also deleted in mip120⁶⁷) are completely female sterile, have much smaller ovaries and the terminal egg chamber development show a consistent arrest, apparently between stages 7 and 8, in all ovarioles. Nurse cells in these egg chambers - unlike those in wild-type control stage 7 and 8 egg chambers - contain large condensed chromosomes, each with a single discrete cid-positive centromere), that have failed to undergo chromosome disassembly and dispersion.

(Cheng et al., 2017)

mip120⁶⁷ mutants (Df(2R)mip120⁶⁷ animals in which EfTuM⁺ function is rescued by EfTuM^+tBa) can survive to adulthood, but they are generally unhealthy and appear smaller than non-mutant siblings. Males die within a day or so of eclosion and females die sporadically within 2-10 days after eclosion. Surviving adults are sterile.

mip120⁶⁷ adults (Df(2R)mip120⁶⁷ animals in which EfTuM⁺ function is rescued by EfTuM^+tBa) have moderate to severe eye phenotypes, including small or completely absent eyes and a rough appearance.

mip120⁶⁷ adults (Df(2R)mip120⁶⁷ animals in which EfTuM⁺ function is rescued by EfTuM^+tBa) completely lack amplification-stage egg chambers, with egg chambers never developing beyond stage 9, even in surviving females 7-10 days after eclosion. In the egg chambers that are present, there appears to be qualitatively less DNA replication in the follicle and nurse cell nuclei than normal (based on the number of BrdU-positive nuclei).

Surviving mip120⁶⁷ adult males (Df(2R)mip120⁶⁷ animals in which EfTuM⁺ function is rescued by EfTuM^+tBa) have smaller testes than control siblings. The testes appear to contain germ cells up to and beyond the spermatocyte stage, including sperm. The mutant sperm are completely immotile.

(Beall et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mip120⁶⁷, mEFTu1^+tBa, mip120[+] is a suppressor | partially of lethal | germline clone | maternal effect | embryonic stage phenotype of lin-52⁹³

(Lewis et al., 2012)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

mip120⁶⁷/Df(2R)BSC274, mEFTu1^+tBa has egg chamber phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/rhi^UAS.GFP

(Cheng et al., 2017)

mip120⁶⁷/Df(2R)BSC274, mEFTu1^+tBa has nurse cell phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/rhi^UAS.GFP

(Cheng et al., 2017)

mip120⁶⁷/Df(2R)BSC274, mEFTu1^+tBa has chromosome | female | adult stage phenotype, non-suppressible by Scer\GAL4^{VP16.nanos.UTR}/rhi^UAS.GFP

(Cheng et al., 2017)

Suppressor of

Statement

Reference

mip120⁶⁷, mEFTu1^+tBa, mip120[+] is a suppressor | partially of embryo | germline clone | maternal effect phenotype of lin-52⁹³

(Lewis et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Neither the arrest of oogenesis nor the persistent undispersed chromosome condensation phenotype in the nurse cells observed in egg chambers from mip120⁶⁷,mEFTu1^+tBa/Df(2R)BSC274 mutant females (carrying mip120⁶⁷ recombined with mEFTu1^+tBa to rescue the mEFTu1 gene also deleted in mip120⁶⁷) is rescued by Scer\GAL4^{nos.UTR.T:Hsim\VP16}-driven expression of rhi^{Scer\UAS.T:Avic\GFP}.

(Cheng et al., 2017)

Approximately 21% of embryos derived from females that carry homozygous lin-52⁹³ mutant germline clones and are heterozygous for mip120⁶⁷ (Df(2R)mip120⁶⁷ mutants in which EfTuM[+] function has been restored using EfTuM^+tBa) crossed to wild type males are able to hatch, a partial suppression of the phenotype seen when females are maternally mutant for lin-52⁹³ alone.

(Lewis et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mip120⁶⁷ is rescued by mip120^FL.mCherry

(Cheng et al., 2017)

mip120⁶⁷ is rescued by mip120^cBa

(Beall et al., 2007)

Not rescued by

mip120⁶⁷ is not rescued by mip120^{Nterm.mCherry}