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General Information
Symbol
Dmel\mip12067
Species
D. melanogaster
Name
FlyBase ID
FBal0216297
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Cytology
    Nature of the lesion
    Statement
    Reference

    Imprecise excision of the P{SUPor-P}mip120KG05422 insertion resulting in a deletion which removes most of the sequences encoding mip120. Sequences encoding the EfTuM gene (which is within an intron of mip120) are completely deleted.

    Caused by aberration
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    mip12067 homozygotes do not show any obvious egg chambers phenotype.

    mip12067,mEFTu1+tBa/Df(2R)BSC274 mutant flies (carrying mip12067 recombined with mEFTu1+tBa to rescue the mEFTu1 gene also deleted in mip12067) are completely female sterile, have much smaller ovaries and the terminal egg chamber development show a consistent arrest, apparently between stages 7 and 8, in all ovarioles. Nurse cells in these egg chambers - unlike those in wild-type control stage 7 and 8 egg chambers - contain large condensed chromosomes, each with a single discrete cid-positive centromere), that have failed to undergo chromosome disassembly and dispersion.

    mip12067 mutants (Df(2R)mip12067 animals in which EfTuM+ function is rescued by EfTuM+tBa) can survive to adulthood, but they are generally unhealthy and appear smaller than non-mutant siblings. Males die within a day or so of eclosion and females die sporadically within 2-10 days after eclosion. Surviving adults are sterile.

    mip12067 adults (Df(2R)mip12067 animals in which EfTuM+ function is rescued by EfTuM+tBa) have moderate to severe eye phenotypes, including small or completely absent eyes and a rough appearance.

    mip12067 adults (Df(2R)mip12067 animals in which EfTuM+ function is rescued by EfTuM+tBa) completely lack amplification-stage egg chambers, with egg chambers never developing beyond stage 9, even in surviving females 7-10 days after eclosion. In the egg chambers that are present, there appears to be qualitatively less DNA replication in the follicle and nurse cell nuclei than normal (based on the number of BrdU-positive nuclei).

    Surviving mip12067 adult males (Df(2R)mip12067 animals in which EfTuM+ function is rescued by EfTuM+tBa) have smaller testes than control siblings. The testes appear to contain germ cells up to and beyond the spermatocyte stage, including sperm. The mutant sperm are completely immotile.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressor of
    Statement
    Reference
    Phenotype Manifest In
    NOT suppressed by
    Suppressor of
    Statement
    Reference
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Neither the arrest of oogenesis nor the persistent undispersed chromosome condensation phenotype in the nurse cells observed in egg chambers from mip12067,mEFTu1+tBa/Df(2R)BSC274 mutant females (carrying mip12067 recombined with mEFTu1+tBa to rescue the mEFTu1 gene also deleted in mip12067) is rescued by Scer\GAL4nos.UTR.T:Hsim\VP16-driven expression of rhiScer\UAS.T:Avic\GFP.

    Approximately 21% of embryos derived from females that carry homozygous lin-5293 mutant germline clones and are heterozygous for mip12067 (Df(2R)mip12067 mutants in which EfTuM[+] function has been restored using EfTuM+tBa) crossed to wild type males are able to hatch, a partial suppression of the phenotype seen when females are maternally mutant for lin-5293 alone.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments

    The female sterility, oogenesis arrest as well as the nurse cell nuclear phenotype (persistent undispersed and condensed chromosomes) characteristic for mip12067,mEFTu1+tBa/Df(2R)BSC274 mutant females (carrying mip12067 recombined with mEFTu1+tBa to rescue the mEFTu1 gene also deleted in mip12067) is rescued by combination with mip120FL.T:Disc\RFP-mCherry but not by combination with any of the following: mip120Nterm.T:Disc\RFP-mCherry, mip120Cterm.T:Disc\RFP-mCherry, mip120CXC.T:Disc\RFP-mCherry or mip120HCH.T:Disc\RFP-mCherry.

    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    Name Synonyms
    Secondary FlyBase IDs
      References (5)