FlyBase Allele Report: Dmel\ens[HP36480]

General Information

Symbol

Dmel\ens^HP36480

Species

D. melanogaster

Name

FlyBase ID

FBal0216583

Feature type

allele

Associated gene

Dmel\ens

Associated Insertion(s)

P{EPg}ens^HP36480

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{EPg}ens^HP36480

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EPg}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:4,133,932..4,133,932 [+]

(Matthews, 2008.4.15)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis

(Gallaud et al., 2014)

abnormal mitotic cell cycle

(Gallaud et al., 2014)

Phenotype Manifest In

larval brain

(Gallaud et al., 2014)

lateral transverse muscle cell (with ens^swo)

(Metzger et al., 2012)

neuroblast

(Gallaud et al., 2014)

spindle

(Gallaud et al., 2014)

Detailed Description

Statement

Reference

ens^HP36480 mutants exhibit small brain lobes and a higher mitotic index than in wild-type, suggesting a mitotic delay due to prolonged spindle assembly checkpoint activation.

ens^HP36480 mutant neuroblast spindles are not fully separated at prophase, and metaphase spindle are shorter than in wild-type. In addition, the time between nuclear envelope breakdown and anaphase onset is longer in ens^HP36480 mutants than in wild-type. Mean spindle length is shorter in ens^HP36480 mutants than in wild-type.

Centrosome splitting after cytokinesis is normal in ens^HP36480 mutant neuroblasts, however separation is incomplete. There is no monopolar spindle formation, suggesting that the machinery required to separate centrosomes at nuclear envelope breakdown is functional.

Approximately 14% of ens^HP36480 mutants display incomplete centrosome separation and severe mis-positioning.

ens^HP36480 mutant neuroblasts, subjected to cold treatment (which leads to microtubule depolymerisation), display weak aster formation around the chromosomes and no obvious Kt fibers after 30 seconds at 25[o]C. After 90 seconds, only short spindles with weak, disorganised microtubule arrays are present. Bipolar spindle assembly occurs within 3 minutes in flies lacking ens^HP36480, as in wild-type.

The mean rate of microtubule polymerisation is significantly lower in ens^HP36480 mutants than in wild-type neuroblasts.

(Gallaud et al., 2014)

ens^HP36480 fails to complement the myonuclear positioning defect of ens^swo.

(Metzger et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ens^HP36480 has abnormal mitotic cell cycle phenotype, enhanceable by Sas-4^s2214

(Gallaud et al., 2014)

ens^HP36480 has abnormal cytokinesis phenotype, enhanceable by Sas-4^s2214

(Gallaud et al., 2014)

Enhancer of

Statement

Reference

ens^HP36480, Scer\GAL4^GMR.PF is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

ens[+]/ens^HP36480 is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

ens^HP36480 is an enhancer of abnormal mitotic cell cycle phenotype of Sas-4^s2214

(Gallaud et al., 2014)

ens^HP36480 is an enhancer of abnormal cytokinesis phenotype of Sas-4^s2214

(Gallaud et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

ens^HP36480 has larval brain phenotype, enhanceable by Sas-4^s2214

(Gallaud et al., 2014)

ens^HP36480 has spindle phenotype, enhanceable by Sas-4^s2214

(Gallaud et al., 2014)

ens^HP36480 has neuroblast phenotype, enhanceable by Sas-4^s2214

(Gallaud et al., 2014)

Enhancer of

Statement

Reference

ens^HP36480, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

ens[+]/ens^HP36480 is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

ens^HP36480 is an enhancer of larval brain phenotype of Sas-4^s2214

(Gallaud et al., 2014)

ens^HP36480 is an enhancer of spindle phenotype of Sas-4^s2214

(Gallaud et al., 2014)

ens^HP36480 is an enhancer of neuroblast phenotype of Sas-4^s2214

(Gallaud et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

In ens^HP36480,Sas-4^s2214 double mutants, mitosis lasts for approximately 10 minutes (longer than in the Sas-4^s2214 single mutant) and the distribution of microtubules is more strongly affected than in the single mutant. The spindles are much shorter than in either single mutant. After cold treatment, which depolymerises microtubules, ens^HP36480,Sas-4^s2214 double mutants exhibit compromised microtubule regrowth, as in ens^HP36480 single mutants. Bipolar spindle assembly is severely compromised in these double mutants.

(Gallaud et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ens^HP36480 is rescued by ens^{Ubi-p63E.EGFP}

(Gallaud et al., 2014)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

21996

w¹¹¹⁸; P{EPg}ens^HP36480

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG14998^HP36480

(Matthews, 2008.4.15)

ens^HP36480

(Feuillette et al., 2020, Metzger et al., 2012)

ens^null

(Gallaud et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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