FlyBase Allele Report: Dmel\Pex2[HP35039]

General Information

Symbol

Dmel\Pex2^HP35039

Species

D. melanogaster

Name

FlyBase ID

FBal0216593

Feature type

allele

Associated gene

Dmel\Pex2

Associated Insertion(s)

P{EPg}Pex2^HP35039

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{EPg}Pex2^HP35039

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{EPg}

misexpression element

Regulatory region(s)

UASp

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:8,340,066..8,340,066 [-]

(Matthews, 2008.4.15)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of peroxisomal disease

CEA

(Chen et al., 2010)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis

(Chen et al., 2010)

abnormal developmental rate

(Chen et al., 2010)

abnormal meiotic cell cycle

(Chen et al., 2010)

male sterile

(Chen et al., 2010)

short lived (with Df(3L)BSC376)

(Wangler et al., 2017)

short lived (with Pex2^{CR70193-KO-kG4})

(Gomez et al., 2025)

viable

(Chen et al., 2010)

Phenotype Manifest In

spermatid

(Chen et al., 2010)

testis

(Chen et al., 2010)

Detailed Description

Statement

Reference

pex2^HP35039 mutant testes contain many spermatocytes and some 'cotton ball'-like spheres but completely lack elongated spermatid bundles and mature sperm. The mutant testes appear much thinner and longer on the seventh day after eclosion than they do immediately after eclosion.

pex2^HP35039 mutants display growth defects, with mutants exhibiting a 2-day delay in eclosion.

pex2^HP35039 mutant testes do not contain hardly any normal mature spermatocytes. Instead, only a few fused spermatocytes with condensed chromosomes are found.

pex2^HP35039 mutant testes do not contain hardly any normal mature spermatocytes. Instead, only a few fused spermatocytes with condensed chromosomes are found. in addition, there are more spermatocytes in pex2^HP35039 mutants than wild-type. In pex2^HP35039 mutants, cysts of 16 spermatocytes without cell-cell boundaries and with large round nuclei are present. This suggests that the spermatocyte growth stage is defective. The majority of spermatocytes do not mature, and some are fused. The fused spermatocytes are able to continue develop but exhibit profound defects in spermatid differentiation. Only a few round spermatids are found compared with wild-type and they often exhibit four or more nuclei associated with a single enlarged mitochondrial derivative, indicating a failure of cytokinesis during meiotic division. Chromosome segregation appears unaffected. Unlike wild-type testes, which have very long spermatid bundles, the few spermatids in pex2^HP35039 mutants, presumably from a cyst, form 'cotton ball'-like spheres. Thus pex2^HP35039 mutant spermatids can initiate the extension of sperm tails, but this process is abnormal.

pex2^HP35039 mutant spermatocytes go through G1/S phase normally but do not reach the G2/M transition.

(Chen et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pex2^HP35039/Df(3L)BSC376 has short lived phenotype, suppressible by Dp(3;2)CH322-53M21

(Wangler et al., 2017)

Suppressor of

Statement

Reference

Pex2^HP35039/CG7081[+] is a suppressor | partially of male sterile phenotype of bond^Z5274

(Chen et al., 2010)

Pex2^HP35039/CG7081[+] is a suppressor | partially of abnormal cytokinesis phenotype of bond^Z5274

(Chen et al., 2010)

Pex2^HP35039/CG7081[+] is a suppressor | partially of male sterile phenotype of bond^Z3437

(Chen et al., 2010)

Pex2^HP35039/CG7081[+] is a suppressor | partially of abnormal cytokinesis phenotype of bond^Z3437

(Chen et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

A pex2^HP35039/+ background partially suppresses the male sterility and cytokinesis defects found in bond^Z3437 mutants.

(Chen et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Pex2^HP35039 is rescued by Scer\GAL4^nanos.PG/Pex2^UAS.cCa

(Chen et al., 2010)

Pex2^HP35039 is rescued by Pex2^UAS.cCa/Scer\GAL4^Hsp83.PA

(Chen et al., 2010)

Pex2^HP35039 is rescued by Scer\GAL4^nanos.PG/Pex2^UAS.mCherry

(Chen et al., 2010)

Pex2^HP35039 is rescued by Scer\GAL4^Hsp83.PA/Pex2^UAS.mCherry

(Chen et al., 2010)

Not rescued by

Pex2^HP35039 is not rescued by Scer\GAL4^ptc-559.1/Pex2^UAS.cCa

(Chen et al., 2010)

Pex2^HP35039 is not rescued by Scer\GAL4^ptc-559.1/Pex2^UAS.mCherry

(Chen et al., 2010)

Comments

The male sterility found in pex2^HP35039 can be rescued through expression of pex2^Scer\UAS.cCa under the control of Scer\GAL4^nos.PG or Scer\GAL4^Hsp83.PA. Expression under the control of Scer\GAL4^ptc-559.1 fails to rescue.

The male sterility found in pex2^HP35039 can be rescued through expression of pex2^{Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^nos.PG or Scer\GAL4^Hsp83.PA. Expression under the control of Scer\GAL4^ptc-559.1 fails to rescue.

(Chen et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

21973

w¹¹¹⁸; P{EPg}Pex2^HP35039/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG7081^HP35039

(Matthews, 2008.4.15)

Pex2^HP35039

(Sellin et al., 2018)

pex2^EP

(Wangler et al., 2017)

pex2^EPg

(Wangler et al., 2017)

pex2^HP35039

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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