glob1^KG06649 homozygous and glob1^KG06649/Df(3R)Exel8162 transheterozygous females show severe decrease in fertility and some are completely sterile.

Ovaries from 3-day-old glob1^KG06649 homozygous females are smaller compared to age-matched controls largely due to decreased number of late-stage follicles, but no obvious defects are observed in the morphology of other reproductive organs. Most of the eggs laid by these females are poorly developed and fragile with shrunken and flaccid dorsal appendages. Up to stage 7 the mutant egg chambers have morphology comparable to wild-type but during the subsequent stages most of the egg chambers attain irregular arrangement of follicle cells with multilayering, especially at the posterior surface (also observed in glob1^KG06649/Df(3R)Exel8162 follicles). In some cases, the nurse cell nuclei form clusters and abnormal positioning of oocytes is also occasionally observed. In about 30% of stage 10 mutant egg chambers the oocyte nucleus is mispositioned, the polar cells are often misplaced too, moreover the 'dumpless' phenotype is also frequent among the mutant follicles. glob1^KG06649 mutant egg chambers also display highly increased apoptotic signal in follicle and nurse cells (TUNEL assay) relative to stage-matched wild-type controls.

Vast majority of glob1^KG06649/glob1^KG06649 follicles show aberrant organization of F-actin with occasional aggregations from stages 6-7 onwards. During stages 10-12 an F-actin 'cage' develops around nurse cell nuclei to restrain its position and this is almost completely missing in ~90% of the mutant egg chambers; it appears that in some cases this leads to nurse cell nuclei actually entering the oocyte along with the cytoplasmic material pumped into it by nurse cells at this stage. glob1^KG06649 follicles also display abnormalities in the distribution of cell adhesion and cell-cell septate junction markers and severe defects in the positioning and morphology of ring canals, in some cases the ring canals are even pushed into the oocyte.

Similarly, in glob1^KG06649 mutant somatic clones in the follicular epithelium, the F-actin is erratically organized, the cells within the clone appear larger and more spherical than the surrounding non-clonal cells and are irregularly arranged.

Mosaic ovaries containing germline glob1^KG06649 clones (created by the DFS/OvoD technique) contain fewer mature eggs and abnormally developing follicles are frequently observed. Females with these mosaic ovaries lay much fewer eggs, about half of them unfertilized (wild-type females lay hardly any unfertilized eggs). Post-stage 7 mosaic egg chambers display disrupted F-actin cytoskeleton in the nurse cells and oocytes, abnormal positioning of the ring canals, and occasionally multiple oocytes within a single follicle (usually two, placed at the opposite ends of the egg chamber).

glob1^KG06649/glob1^KG06649 as well as glob1^KG06649/Df(3R)Exel8162 mutants display partial lethality with majority of homozygotes dying during embryonic stage, high mortality is observed also in larval stage. Only about a fifth of glob1^KG06649 and 4% of glob1^KG06649/Df(3R)Exel8162 transheterozygotes survive to adulthood. glob1^KG06649 homozygotes as well as glob1^KG06649/Df(3R)Exel8162 frequently show melanotic masses, visible especially during larval stages.

glob1^KG06649/glob1^KG06649 animals are developmentally delayed and first instar larvae display defects in tracheal liquid clearance as well as the tracheal morphology.

glob1^KG06649/glob1^KG06649 adults show age-progressive decline in climbing abilities that is more pronounce than in wild-type controls and a decrease in adult life span accompanied by aging associated impairments.