UASt regulatory sequences drive expression of the kon genomic region (missing the first 39 amino acids) which has a Tag:SS(wg) signal sequence and three copies of a Tag:HA tag at the N-terminal end of the coding sequence. A V to G mutation has been introduced into the last conserved amino acid residue in the PDZ-binding motif.
In embryos expressing konVG.Scer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4Mef2.PR, some ectopic filopodia are seen at muscle tips at much later stages than normal.
Expression of konVG.Scer\UAS.T:Ivir\HA1,T:wg under the control of Scer\GAL4Bx-MS1096 has no effect on wing development.
Scer\GAL4tey-5053A/konVG.UAS.Tag:HA,Tag:SS(wg) partially rescues konC452/konC41