The AU[[4-6]] motifs in the "pA2" polyadenylation signal in ewg intron 6 have been mutated to A(CU)[[2-3]].
Splicing of intron 6 in ewgδ7.m1-3.elav mutants is abolished in photoreceptors and in adult brains.
The ability of ewgδ7.m1-3.elav transgenes is only ~42% and is comparable to a deletion of exon J, demonstrating that elav function in ewg intron 6 processing is abolished in this mutant.