FlyBase Allele Report: Dmel\AIF[ΔN.UAS.Tag:MYC]

General Information

Symbol

Dmel\AIF^{ΔN.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0218259

Feature type

allele

Associated gene

Dmel\AIF

Associated Insertion(s)

Carried in Construct

P{UAS-AIF.ΔN.myc}

Key Links

Transgenic product class

inframe_deletion

(Joza et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Joza et al., 2008)

Mutagen

in vitro construct

(Joza et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-AIF.ΔN.myc}

Cytology

Description

UASt regulatory sequences drive expression of an N-terminally deleted form of AIF (amino acids 1-176 are missing) which is tagged at the C-terminal end with Tag:MYC.

(Joza et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

AIF

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^ey.PH

(Joza et al., 2008)

partially lethal, with Scer\GAL4^ey.PH

(Joza et al., 2008)

visible, with Scer\GAL4^ey.PH

(Joza et al., 2008)

visible, with Scer\GAL4^pnr-MD237

(Joza et al., 2008)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Joza et al., 2008)

eye disc, with Scer\GAL4^ey.PH

(Joza et al., 2008)

mesothoracic bristle, with Scer\GAL4^pnr-MD237

(Joza et al., 2008)

ommatidium, with Scer\GAL4^ey.PH

(Joza et al., 2008)

Detailed Description

Statement

Reference

Flies expressing AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH show a marked reduction in eye size and have disorganised ommatidial architecture. This phenotype shows 100% penetrance but variable expressivity, ranging from roughened appearance and normal size to a severe reduction in size. The eye discs of larvae expressing AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH show ectopic cell death.

Expression of AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^pnr-MD237 results in a reduction in bristle number and size on the dorsal thorax.

(Joza et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has visible phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ey.PH

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^pnr-MD237 has visible phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^pnr-MD237

(Joza et al., 2008)

Suppressed by

Statement

Reference

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has visible phenotype, suppressible | partially by Diap1^UAS.cMb, Scer\GAL4^ey.PH

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has visible phenotype, suppressible | partially by Diap2^UAS.cMa, Scer\GAL4^ey.PH

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has visible phenotype, suppressible by Trx2^GD10505, Scer\GAL4^ey.PH

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has partially lethal phenotype, suppressible by Trx2^GD10505, Scer\GAL4^ey.PH

(Joza et al., 2008)

NOT suppressed by

Statement

Reference

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by Dark^CD4/Dark^CD4

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by p53^-ns/p53^-ns

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by Dredd^B118

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has visible phenotype, non-suppressible by Df(3L)H99/+

(Joza et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has eye phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ey.PH

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^pnr-MD237 has mesothoracic bristle phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^pnr-MD237

(Joza et al., 2008)

Suppressed by

Statement

Reference

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has eye phenotype, suppressible by Trx2^GD10505, Scer\GAL4^ey.PH

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by Diap1^UAS.cMb, Scer\GAL4^ey.PH

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by Diap2^UAS.cMa, Scer\GAL4^ey.PH

(Joza et al., 2008)

NOT suppressed by

Statement

Reference

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by Dark^CD4/Dark^CD4

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by p53^-ns/p53^-ns

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by Dredd^B118

(Joza et al., 2008)

AIF^{ΔN.UAS.Tag:MYC}, Scer\GAL4^ey.PH has eye phenotype, non-suppressible by Df(3L)H99/+

(Joza et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The mutant eye phenotype caused by expression of AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH is not ameliorated by Dark^CD4/Dark^CD4, Dredd^B118/Y or Df(3L)H99/+.

The mutant eye phenotype caused by expression of AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH is not ameliorated if they are also homozygous for p53^-ns.

The reduced eye size caused by expression of AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH is significantly, but not completely, suppressed by co-expression of either Diap1^Scer\UAS.cMb or Diap2^Scer\UAS.cMa.

The mutant eye phenotype caused by expression of AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH is completely suppressed by co-expression of Trx-2^GD10505.

(Joza et al., 2008)

Xenogenetic Interactions

Statement

Reference

The reduction in eye size caused by expression of AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ey.PH is enhanced by co-expression of BacA\p35^Scer\UAS.cHa.

The loss of thoracic bristles caused by expression of AIF^{ΔN.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^pnr-MD237 is enhanced by co-expression of BacA\p35^Scer\UAS.cHa.

(Joza et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

AIF^{ΔN.Scer\UAS.T:Hsap\MYC}

AIF^{ΔN.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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