dec-1fc106ΔV288-E473 is a dominant female sterile mutation that can be used as an eggshell marker for complete follicle cell clones of a mutation of interest; FRT-mediated recombination in females transheterozygous for a mutation of interest and dec-1fc106ΔV288-E473 can generate egg chambers containing homozygous mutant follicle cells that lack dec-1fc106ΔV288-E473. Eggs derived from egg chambers with large or complete mutant follicle cell clones are readily identifiable because they remain intact, whereas those produced by non-mutant follicular epithelia retaining dec-1fc106ΔV288-E473 collapse.
dec-1fc106ΔV288-E473 is a dominant female sterile mutation that can be used as an eggshell marker for complete follicle cell clones of a mutation of interest; FRT-mediated recombination in females transheterozygous for a mutation of interest and dec-1fc106ΔV288-E473 can generate egg chambers containing homozygous mutant follicle cells that lack dec-1fc106ΔV288-E473. Eggs derived from egg chambers with large or complete mutant follicle cell clones are readily identifiable because they remain intact, whereas those produced by non-mutant follicular epithelia retaining dec-1fc106ΔV288-E473 collapse.