FlyBase Allele Report: Dmel\Fs[UAS.cPa]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\Fs^UAS.cPa

General Information

Symbol

Dmel\Fs^UAS.cPa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Pentek

FlyBase ID

FBal0221225

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

Also Known As

UAS-fs

Key Links

Transgenic product class

(Pentek et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

(Pentek et al., 2009)

Mutagen

in vitro construct

(Pentek et al., 2009)

Progenitor genotype

Carried in construct

Cytology

Description

UASt regulatory sequences drive expression of Fs.

(Pentek et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | embryonic stage, with Scer\GAL4^elav-C155

(Pentek et al., 2009)

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

(Pentek et al., 2009)

decreased cell number | progressive | spermatogenesis, with Scer\GAL4^NP1624

(Herrera et al., 2021)

fertile, with Scer\GAL4^Mef2.PR

(Pentek et al., 2009)

lethal | pharate adult stage, with Scer\GAL4^69B

(Pentek et al., 2009)

lethal | pharate adult stage, with Scer\GAL4^A9

(Pentek et al., 2009)

lethal | pharate adult stage, with Scer\GAL4^arm.PS

(Pentek et al., 2009)

lethal | pharate adult stage, with Scer\GAL4^elav-C155

(Pentek et al., 2009)

lethal | pharate adult stage, with Scer\GAL4^nrv2.PS

(Pentek et al., 2009)

lethal | P-stage, with Scer\GAL4^69B

(Pentek et al., 2009)

lethal | P-stage, with Scer\GAL4^A9

(Pentek et al., 2009)

lethal | P-stage, with Scer\GAL4^arm.PS

(Pentek et al., 2009)

lethal | P-stage, with Scer\GAL4^elav-C155

(Pentek et al., 2009)

lethal | P-stage, with Scer\GAL4^nrv2.PS

(Pentek et al., 2009)

viable, with Scer\GAL4^Mef2.PR

(Pentek et al., 2009)

Phenotype Manifest In

air bubble, with Scer\GAL4^69B

(Pentek et al., 2009)

air bubble, with Scer\GAL4^A9

(Pentek et al., 2009)

air bubble, with Scer\GAL4^arm.PS

(Pentek et al., 2009)

air bubble, with Scer\GAL4^elav-C155

(Pentek et al., 2009)

air bubble, with Scer\GAL4^nrv2.PS

(Pentek et al., 2009)

anterior pupal spiracle, with Scer\GAL4^69B

(Pentek et al., 2009)

anterior pupal spiracle, with Scer\GAL4^A9

(Pentek et al., 2009)

anterior pupal spiracle, with Scer\GAL4^arm.PS

(Pentek et al., 2009)

anterior pupal spiracle, with Scer\GAL4^elav-C155

(Pentek et al., 2009)

anterior pupal spiracle, with Scer\GAL4^nrv2.PS

(Pentek et al., 2009)

axon & eye photoreceptor cell | larval stage, with Scer\GAL4^elav-C155

(Pentek et al., 2009)

embryonic/larval optic lobe, with Scer\GAL4^elav-C155

(Pentek et al., 2009)

hub cell | decreased number, with Scer\GAL4^NP1624

(Herrera et al., 2021)

larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4^elav-C155

(Pentek et al., 2009)

leg | pharate adult stage, with Scer\GAL4^69B

(Pentek et al., 2009)

leg | pharate adult stage, with Scer\GAL4^A9

(Pentek et al., 2009)

leg | pharate adult stage, with Scer\GAL4^arm.PS

(Pentek et al., 2009)

leg | pharate adult stage, with Scer\GAL4^elav-C155

(Pentek et al., 2009)

leg | pharate adult stage, with Scer\GAL4^nrv2.PS

(Pentek et al., 2009)

pupa, with Scer\GAL4^69B

(Pentek et al., 2009)

pupa, with Scer\GAL4^A9

(Pentek et al., 2009)

pupa, with Scer\GAL4^arm.PS

(Pentek et al., 2009)

pupa, with Scer\GAL4^elav-C155

(Pentek et al., 2009)

pupa, with Scer\GAL4^nrv2.PS

(Pentek et al., 2009)

wing, with Scer\GAL4^A9

(Pentek et al., 2009)

Detailed Description

Statement

Reference

The expression of Fs^UAS.cPa under the control of Scer\GAL4^NP1624 alone leads to a progressive decrease in the number of hub cells in the testes.

(Herrera et al., 2021)

Expression of Fs^Scer\UAS.cPa under the control of Scer\GAL4^Mef2.PR results 33% of animals pupariating in the food, but 100% of the animals eclose.

Expression of Fs^Scer\UAS.cPa under the control of Scer\GAL4^arm.PS results in a number of defects; 43% of animals pupariate in the food, 65% of pupae are asymmetric, 84% of pupae fail to evert anterior spiracles, 65% of pupae have gas bubble defects, 25% of animals show pharate adult lethality, 71% of the pharate adults have crooked legs and only 1% of the animals eclose.

Expression of Fs^Scer\UAS.cPa under the control of Scer\GAL4^69B results in a number of defects; 86% of animals pupariate in the food, 84% of pupae are asymmetric, 66% of pupae fail to evert anterior spiracles, 59% of pupae have gas bubble defects, 5% of animals show pharate adult lethality, 100 % of the pharate adults have crooked legs and none of the animals eclose.

Expression of Fs^Scer\UAS.cPa under the control of Scer\GAL4^A9 results in a number of defects; 96% of animals pupariate in the food, 38% of pupae are asymmetric, 69% of pupae fail to evert anterior spiracles, 37% of pupae have gas bubble defects, 49% of animals show pharate adult lethality, 50% of the pharate adults have crooked legs and only 1% of the animals eclose.

Expression of Fs^Scer\UAS.cPa under the control of Scer\GAL4^elav-C155 results in a number of defects; 99% of animals pupariate in the food, 86% of pupae are asymmetric, 49% of pupae fail to evert anterior spiracles, 70% of pupae have gas bubble defects, 5% of animals show pharate adult lethality, 75% of the pharate adults have crooked legs and none of the animals eclose.

Expression of Fs^Scer\UAS.cPa under the control of Scer\GAL4^nrv2.PS results in a number of defects; 88% of animals pupariate in the food, 83% of pupae are asymmetric, 90% of pupae fail to evert anterior spiracles, 60% of pupae have gas bubble defects, 11% of animals show pharate adult lethality, 78% of the pharate adults have crooked legs and none of the animals eclose.

At 18[o]C, 69% of animals expressing Fs^Scer\UAS.cPa under the control of Scer\GAL4^A9 are viable. The wing area of these adults is reduced (83 +/- 5%) compared to controls, but the wing is patterned normally.

Expression of Fs^Scer\UAS.cPa under the control of Scer\GAL4^elav-C155 results in ISNb axon pathfinding defects in embryos, including stalling of the ISNb axons prior to muscles 12 and 13.

Larvae expressing Fs^Scer\UAS.cPa under the control of Scer\GAL4^elav-C155 have smaller optic lobes than normal and photoreceptor axons fail to properly innervate the optic lobe.

(Pentek et al., 2009)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Fs^UAS.cPa, Scer\GAL4^A9 has lethal phenotype, suppressible | partially by Actβ^UAS.cZa, Scer\GAL4^A9

(Pentek et al., 2009)

Fs^UAS.cPa, Scer\GAL4^A9 has lethal phenotype, suppressible | partially by daw^UAS.cPa, Scer\GAL4^A9

(Pentek et al., 2009)

NOT suppressed by

Statement

Reference

Fs^UAS.cPa, Scer\GAL4^A9 has lethal phenotype, non-suppressible by gbb^UAS.cKa, Scer\GAL4^A9

(Pentek et al., 2009)

Fs^UAS.cPa, Scer\GAL4^A9 has lethal phenotype, non-suppressible by scw^UAS.cNa, Scer\GAL4^A9

(Pentek et al., 2009)

Suppressor of

Statement

Reference

Fs^UAS.cPa, Scer\GAL4^NP1624, Scer\GAL80^{hh-MI10526-T3XG80.0} is a suppressor of decreased cell number | spermatogenesis phenotype of Dp^GD4444, Scer\GAL4^NP1624, Scer\GAL80^{hh-MI10526-T3XG80.0}

(Herrera et al., 2021)

Phenotype Manifest In

Suppressor of

Statement

Reference

Fs^UAS.cPa, Scer\GAL4^NP1624, Scer\GAL80^{hh-MI10526-T3XG80.0} is a suppressor of hub cell | decreased number phenotype of Dp^GD4444, Scer\GAL4^NP1624, Scer\GAL80^{hh-MI10526-T3XG80.0}

(Herrera et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^upd1-E132/Fs^UAS.cPa rescues Fs^null

(Herrera et al., 2021)

Scer\GAL4^fng-11-26/Fs^UAS.cPa rescues Fs^null

(Herrera et al., 2021)

Partially rescues

Scer\GAL4^NP1624/Fs^UAS.cPa partially rescues Fs^GD15843

(Herrera et al., 2021)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

606386

y¹ w^*; P{UAS-Fs.P}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Fs^Scer\UAS.cPa

Fs^UAS.cPa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Pentek

Secondary FlyBase IDs

References (2)

Report Sections