UASt regulatory sequences drive expression of sns, in which eleven candidate sites for phosphorylation of tyrosine have been mutated (amino acid replacements Y1133F, Y1152F, Y1162F, Y1174F, Y1186F, Y1196F, Y1233F, Y1247F, Y1248F, Y1255F and Y1258F). The coding sequence is tagged at the C-terminal end with a single Tag:HA tag.
The myoblast fusion defects of snsXB3/snsZF1.4 embryos are rescued by expression of snsF11.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Mef2.PR.