FlyBase Allele Report: Dmel\mars[UAS.Tag:FLAG]

General Information

Symbol

Dmel\mars^UAS.Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0230464

Feature type

allele

Associated gene

Dmel\mars

Associated Insertion(s)

Carried in Construct

P{UAS-mars.Flag}

Also Known As

pUAST-Flag-mars

Key Links

Transgenic product class

wild_type

(Yang et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Yang et al., 2005)

Mutagen

in vitro construct

(Yang et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-mars.Flag}

Cytology

Description

UASt sequences drive expression of full length mars which is tagged at the N-terminal end with Tag:FLAG.

(Yang et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mars

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^GMR.PF

(Yang et al., 2005)

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Yang et al., 2005)

increased cell death, with Scer\GAL4^GMR.PF

(Yang et al., 2005)

lethal, with Scer\GAL4^ap-md544

(Yang et al., 2005)

lethal, with Scer\GAL4^dpp.blk1

(Yang et al., 2005)

lethal, with Scer\GAL4^ey.PH

(Yang et al., 2005)

lethal, with Scer\GAL4^ptc-559.1

(Yang et al., 2005)

visible, with Scer\GAL4^GMR.PF

(Yang et al., 2005)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Yang et al., 2005)

eye disc, with Scer\GAL4^GMR.PF

(Yang et al., 2005)

Detailed Description

Statement

Reference

Flies expressing mars^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF have small, rough eyes. This phenotype is partially suppressed by coexpression of mars^Scer\UAS.WIZ.

The number of mitotic cells at the second mitotic wave in eye discs expressing mars^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF is increased compared to controls. The distribution of BrdU labeling is similar in the mutant and control eye discs.

68.8 +/- 9.5% of mitotic figures at the second mitotic wave are abnormal in eye discs expressing mars^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF. Circular or fragmented chromosomes, misalignment of chromosomes, aberrant anaphase with lagging chromosomes and incorrect separation of centriole pairs is seen.

The fraction of mitotic cells in anaphase or telophase is reduced to 2.7 +/- 1.8% in eye discs expressing mars^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF (compared to 21.0 +/- 11.0% of mitotic cells being in anaphase or telophase in wild-type discs).

Eye discs expressing mars^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF show a significant increase in the number of apoptotic cells posterior to the morphogenetic furrow compared to wild-type eye discs.

(Yang et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, mars^UAS.Tag:FLAG has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PF

(Yang et al., 2005)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The increased cell death posterior to the morphogenetic furrow that is seen in eye discs expressing mars^{Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of BacA\p35^Scer\UAS.cHa.

(Yang et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mars^{Scer\UAS.T:Zzzz\FLAG}

mars^UAS.Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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