FlyBase Allele Report: Dmel\scrib[KK101128]

General Information

Symbol

Dmel\scrib^KK101128

Species

D. melanogaster

Name

FlyBase ID

FBal0231079

Feature type

allele

Associated gene

Dmel\scrib

Associated Insertion(s)

Carried in Construct

P{KK101128}

Also Known As

UAS-scrib-RNAi, UAS-scrib^RNAi, scrib-i

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101128}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101128

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Tamori et al., 2016)

CEA with Stat92E^{ΔNΔC.UASp.Tag:HA}, RhoGEF2^HMS01118

(Tamori et al., 2016)

CEA with Ras85D^V12.UAS

(Muzzopappa et al., 2017)

model of leukemia

CEA with Ras85D^V12.UAS

(Arefin et al., 2017)

model of carcinoma

CEA with Ras85D^V12.UAS

(Murcia et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cancer

is exacerbated by Stat92E^{ΔNΔC.UASp.Tag:HA}

(Tamori et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

wing disc | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PI

(Tamori et al., 2016)

wing hinge primordium | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PI

(Tamori et al., 2016)

wing hinge primordium | somatic clone | third instar larval stage, with Scer\GAL4^sd-SG29.1

(Tamori et al., 2016)

Detailed Description

Statement

Reference

Expression of scrib^KK101128 under the control of Scer\GAL4^Hml.Δ does not lead to a significant difference in survival as compared to controls when larvae are infected with the nematode Heterorhabditis bacteriophora and its associated bacterium Photorhabdus luminescens.

(Arefin et al., 2017)

Expression of scrib^KK101128 under the control of Scer\GAL4^ple.PF or Scer\GAL4^GMR13F02 (in combination with a Dicer-2 transgene to enhance RNAi efficiency) results in enhanced memory after conditioning in an olfactory conditioning assay.

(Cervantes-Sandoval et al., 2016)

The expression of scrib^KK101128 under the control of Scer\GAL4^sd-SG29.1 leads to dysplastic overgrowths only in the wing disc hinge region, as compared to controls.

The clonal expression of scrib^KK101128 under the control of Scer\GAL4^Act5C.PI (together with Dicer-2, for efficient RNAi) within mosaic third instar larval wing discs leads to dysplastic overgrowths only in the hinge region and leads to apoptosis (Dcp1 staining) in some scrib^KK101128-expressing cells in both the wing pouch and hinge regions of the mosaic wing disc, particularly at the boundary with non-expressing cells, as compared to controls; these overgrowths are particularly prevalent at the dorsal hinge region and, within it, at medial fold.

(Tamori et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Act5C.PI, scrib^KK101128 has neoplasia | third instar larval stage | somatic clone phenotype, enhanceable by Scer\GAL4^Act5C.PI/Stat92E^{ΔNΔC.UASp.Tag:HA}

(Tamori et al., 2016)

Suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^ap-md544, scrib^KK101128 has increased cell number | larval stage phenotype, suppressible by rpr^UAS.C/Scer\GAL4^He.PZ

(Muzzopappa et al., 2017)

NOT suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^ap-md544, scrib^KK101128 has neoplasia | larval stage phenotype, non-suppressible by rpr^UAS.C/Scer\GAL4^He.PZ

(Muzzopappa et al., 2017)

Enhancer of

Statement

Reference

Ras85D^V12.UAS/scrib^KK101128, Scer\GAL4^hh.PU is an enhancer of increased cell death | third instar larval stage phenotype of Scer\GAL4^hh.PU, rl^GD4697

(Murcia et al., 2019)

scrib^KK101128, Scer\GAL4^Hml.Δ is an enhancer of increased cell number | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.Δ

(Arefin et al., 2017)

NOT Enhancer of

Statement

Reference

scrib^KK101128, Scer\GAL4^Hml.Δ is a non-enhancer of abnormal locomotor behavior | larval stage | conditional phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.Δ

(Arefin et al., 2017)

Suppressor of

Statement

Reference

Ras85D^V12.UAS/scrib^KK101128, Scer\GAL4^hh.PU is a suppressor | conditional of increased cell death | third instar larval stage phenotype of Scer\GAL4^hh.PU, rl^GD4697

(Murcia et al., 2019)

NOT Suppressor of

Statement

Reference

scrib^KK101128, Scer\GAL4^Hml.Δ is a non-suppressor of abnormal locomotor behavior | larval stage | conditional phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.Δ

(Arefin et al., 2017)

Other

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^hh.PU, scrib^KK101128 has abnormal DNA repair | third instar larval stage phenotype

(Murcia et al., 2019)

Ras85D^V12.UAS, Scer\GAL4^Hml.Δ, scrib^KK101128 has abnormal immune response | larval stage phenotype

(Arefin et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^Hml.Δ, scrib^KK101128 has lethal - all die before end of P-stage phenotype

(Arefin et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, scrib^KK101128 has increased cell number | larval stage phenotype

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, scrib^KK101128 has neoplasia | larval stage phenotype

(Muzzopappa et al., 2017)

RhoGEF2^HMS01118, Scer\GAL4^Act5C.PI, Stat92E^{ΔNΔC.UASp.Tag:HA}, scrib^KK101128 has neoplasia | somatic clone | third instar larval stage phenotype

(Tamori et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Act5C.PI, scrib^KK101128 has wing disc | third instar larval stage | somatic clone phenotype, enhanceable by Scer\GAL4^Act5C.PI/Stat92E^{ΔNΔC.UASp.Tag:HA}

(Tamori et al., 2016)

Scer\GAL4^Act5C.PI, scrib^KK101128 has wing hinge primordium | third instar larval stage | somatic clone phenotype, enhanceable by Scer\GAL4^Act5C.PI/Stat92E^{ΔNΔC.UASp.Tag:HA}

(Tamori et al., 2016)

Suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^ap-md544, scrib^KK101128 has embryonic/larval hemocyte | increased number | larval stage phenotype, suppressible by rpr^UAS.C/Scer\GAL4^He.PZ

(Muzzopappa et al., 2017)

NOT suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^ap-md544, scrib^KK101128 has wing disc phenotype, non-suppressible by rpr^UAS.C/Scer\GAL4^He.PZ

(Muzzopappa et al., 2017)

Enhancer of

Statement

Reference

Ras85D^V12.UAS/scrib^KK101128, Scer\GAL4^hh.PU is an enhancer of wing disc | third instar larval stage phenotype of Scer\GAL4^hh.PU, rl^GD4697

(Murcia et al., 2019)

scrib^KK101128, Scer\GAL4^Hml.Δ is an enhancer of embryonic/larval hemocyte | larval stage | increased number phenotype of Ras85D^V12.UAS, Scer\GAL4^Hml.Δ

(Arefin et al., 2017)

Suppressor of

Statement

Reference

Ras85D^V12.UAS/scrib^KK101128, Scer\GAL4^hh.PU is a suppressor | conditional of wing disc | third instar larval stage phenotype of Scer\GAL4^hh.PU, rl^GD4697

(Murcia et al., 2019)

Other

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^hh.PU, scrib^KK101128 has wing disc | third instar larval stage phenotype

(Murcia et al., 2019)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, scrib^KK101128 has wing disc phenotype

(Muzzopappa et al., 2017)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, scrib^KK101128 has myoblast | increased number | larval stage phenotype

(Muzzopappa et al., 2017)

RhoGEF2^HMS01118, Scer\GAL4^Act5C.PI, Stat92E^{ΔNΔC.UASp.Tag:HA}, scrib^KK101128 has wing pouch | somatic clone | third instar larval stage phenotype

(Tamori et al., 2016)

RhoGEF2^HMS01118, Scer\GAL4^Act5C.PI, scrib^KK101128 has wing disc | somatic clone | third instar larval stage phenotype

(Tamori et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The double expression of Ras85D^V12.UAS and scrib^KK101128 for 3 days during larval development under the combined control of Scer\GAL4^hh.PU and Gal80[ts] results in increased DNA damage (comet assay) and decreased DNA damage response (phospho-H2Av) in third instar larval wing discs.

(Murcia et al., 2019)

Co-expression of scrib^KK101128 exacerbates the increased hemocyte count observed in larvae expressing Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Hml.Δ; these larvae are viable up to pupal stage and prepare for pupation, but only a subset of these pupae successfully eclose at 25[o]C, as compared to controls. None of these flies eclose when reared at 29[o]C, and pupae exhibit dessication and an absence of precursors of adult structures, in contrast to controls.

Larvae expressing both Ras85D^V12.Scer\UAS and scrib^KK101128 under the control of Scer\GAL4^Hml.Δ exhibit no significant difference in wound healing; larvae wounded and infected with the bacteria Staphylococcus aureus, Erwinia carotovora, or E.coli survive equally well as controls; and hemocytes isolated from larvae exhibit normal phagocytosis levels upon exposure to to heat killed E. coli, as compared to controls.

Larvae expressing both Ras85D^V12.Scer\UAS and scrib^KK101128 under the control of Scer\GAL4^Bx-MS1096 infected with the nematode Heterorhabditis bacteriophora and its associated bacterium Photorhabdus luminescens, do not show a significant difference in survival as compared to controls; but when these constructs are driven under the control of Scer\GAL4^Hml.Δ, infected larvae show a significant increase in mortality as compared to controls, and also show a similar decrease in motility upon nematode infection as seen in those expressing only Ras85D^V12.Scer\UAS.

(Arefin et al., 2017)

The co-expression of Ras85D^V12.UAS and scrib^KK101128 under the control of Scer\GAL4^ap-md544 induces wing disc neoplasia which, when transplanted, can form solid tumors in the host; in both wing discs and allografts, there is amplification of the resident myoblast population, as well as accumulation of circulating hemocytes. The same tumor induction and hemocyte recruitment occurs if expression is only induced in wing disc transplants (expression induction is controlled by Gal80[ts] and temperature-shift). Hemocyte ablation on the host (by expressing rpr^UAS.C under the control of Scer\GAL4^He.PZ) does not suppress the tumor-like growth.

(Muzzopappa et al., 2017)

The wing disc hinge overgrowths induced by the clonal expression of scrib^KK101128 under the control of Scer\GAL4^Act5C.PI (together with Dicer-2, for efficient RNAi) are enhanced by the co-expression of Stat92E^{ΔNΔC.UASp.Tag:HA}, leading to large tumor masses; this co-expression still does not lead to overgrowths in the wing pouch area and does not affect the basal delamination/extrusion observed upon scrib^KK101128 single expression.

The clonal co-expression of scrib^KK101128 and RhoGEF2^HMS01118 under the control of Scer\GAL4^Act5C.PI (together with Dicer-2, for efficient RNAi) in third instar larval wing discs leads to a significant proportion of apical delamination, which does not occur upon scrib^KK101128 single expression; the additional co-expression of Stat92E^{ΔNΔC.UASp.Tag:HA} leads to overgrowths in the wing pouch domain, which does not occur upon scrib^KK101128 single expression or scrib^KK101128, RhoGEF2^HMS01118 double expression.

(Tamori et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v105412

P{KK101128}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

scrib^KK101128

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

Open Close