FlyBase Allele Report: Dmel\GckIII[KK101915]

General Information

Symbol

Dmel\GckIII^KK101915

Species

D. melanogaster

Name

FlyBase ID

FBal0231558

Feature type

allele

Associated gene

Dmel\GckIII

Associated Insertion(s)

Carried in Construct

P{KK101915}

Also Known As

GckIII RNAi, UAS-GckIII RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK101915}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK101915

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

terminal tracheal cell | third instar larval stage, with Scer\GAL4^btl.PS

(Song et al., 2013)

Detailed Description

Statement

Reference

Expressing GckIII^KK101915 under the control of Scer\GAL4^C587 (together with Dicer-2, for a more efficient RNAi) does not induce any obvious L3 spermatogenesis defects.

(La Marca et al., 2019)

Third instar larvae expressing GckIII^KK101915 under the control of Scer\GAL4^btl.PS exhibit a tracheal morphogenesis phenotype, with the terminal cells displaying a transition zone tube dilation defect. Ectopic septate junctions are seen in these terminal cells; the frequency with which septate junctions extend into the terminal cell transition zone is increased compared to wild type, with junctions extending into between 32% (vari staining) and 42% (Fas3 or cora staining) of cells compared to only 4% in wild type. Additional terminal cells display punctate septate junction staining and short discontinuous patches of septate junction staining can be seen 10s or 100s of microns away from the transition zone.

(Song et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

GckIII^KK101915, Scer\GAL4^btl.PS has terminal tracheal cell | third instar larval stage phenotype, enhanceable by Df(3R)Exel6174/+

(Song et al., 2013)

GckIII^KK101915, Scer\GAL4^btl.PS has terminal tracheal cell | third instar larval stage phenotype, enhanceable by Ccm3[+]/Ccm3^ins

(Song et al., 2013)

NOT Enhanced by

Statement

Reference

GckIII^KK101915, Scer\GAL4^btl.PS has terminal tracheal cell | third instar larval stage phenotype, non-enhanceable by Ccm3[+]/Ccm3^rev

(Song et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

There is a significant reduction in chromosome instability tolerance induced by mad2^GD16427 expression, when GckIII^KK101915 is expressed in imaginal wing discs under the control of Scer\GAL4^en.PU or Scer\GAL4^hh.PU.

(Wong et al., 2014)

Heterozygosity for Df(3R)Exel6174 enhances the penetrance of the transition zone tube dilation defect seen in third instar larval trachea expressing GckIII^KK101915 under the control of Scer\GAL4^btl.PS.

Heterozygosity for Ccm3^ins enhances the penetrance of the transition zone tube dilation defect seen in third instar larval trachea expressing GckIII^KK101915 under the control of Scer\GAL4^btl.PS.

Heterozygosity for Ccm3^rev does not enhance the penetrance of the transition zone tube dilation defect seen in third instar larval trachea expressing GckIII^KK101915 under the control of Scer\GAL4^btl.PS.

(Song et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v107158

P{KK101915}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

GckIII^KK101915

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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