FlyBase Allele Report: Dmel\CalpA[KK104532]

General Information

Symbol

Dmel\CalpA^KK104532

Species

D. melanogaster

Name

FlyBase ID

FBal0233044

Feature type

allele

Associated gene

Dmel\CalpA

Associated Insertion(s)

Carried in Construct

P{KK104532}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK104532}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK104532

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Huntington's disease

CEC with Hsap\HTT^GMR.Q120

(Menzies et al., 2015)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^GMR.Q120

(Menzies et al., 2015)

modeled by Hsap\HTT^{Q46.ex1p.UAS.EGFP}

(Menzies et al., 2015)

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG}

(Menzies et al., 2015)

ameliorates Alzheimer's disease

modeled by Hsap\MAPT^UAS.cWa

(Menzies et al., 2015)

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Park et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

CalpA^KK104532, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG}

(Menzies et al., 2015)

CalpA^KK104532, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

CalpA^KK104532, Scer\GAL4^GMR.PF is a suppressor of abnormal size phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^GMR.PF, CalpA^KK104532/Atg8a^KG07569 is a non-suppressor of visible phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

Scer\GAL4^GMR.PF, CalpA^KK104532/Atg8a^KG07569 is a non-suppressor of abnormal size phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^GMR.PF, CalpA^KK104532/Atg8a^KG07569 is an enhancer of rhabdomere | progressive phenotype of Hsap\HTT^GMR.Q120, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

Suppressor of

Statement

Reference

CalpA^KK104532, Scer\GAL4^GMR.PF is a suppressor of rhabdomere | progressive phenotype of Hsap\HTT^GMR.Q120, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

CalpA^KK104532, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\HTT^{Q46.ex1p.UAS.EGFP}, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

CalpA^KK104532, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG}

(Menzies et al., 2015)

CalpA^KK104532, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

NOT Suppressor of

Statement

Reference

Dcr-2^UAS.cDa, CalpA^KK104532, Atg8a^KG07569, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\HTT^{Q46.ex1p.UAS.EGFP}, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

Scer\GAL4^GMR.PF, CalpA^KK104532/Atg8a^KG07569 is a non-suppressor of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Menzies et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of CalpA^KK104532 results in a clear reduction in the number of aggregates observed in the eyes of flies expressing Scer\GAL4^GMR.PF>Hsap\HTT^{Q46.ex1p.Scer\UAS.T:Avic\GFP-EGFP}.

Co-expression of Scer\GAL4^GMR.PF>CalpA^KK104532 partially suppresses the toxicity of Hsap\HTT^GMR.Q120 in the eye.

Co-expression of CalpA^KK104532 leads to a significant reduction in the number of flies with black necrotic-like spots in the eye, in both males and females expressing Scer\GAL4^GMR.PF>Zzzz\CAG^{Q48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG}.

Co-expression of CalpA^KK104532 in an Atg8a^KG07569 mutant genetic background fails to reduce the number of aggregates observed in the eyes of flies expressing Scer\GAL4^GMR.PF>Hsap\HTT^{Q46.ex1p.Scer\UAS.T:Avic\GFP-EGFP}.

Co-expression of CalpA^KK104532 in an Atg8a^KG07569 mutant genetic background exacerbates the toxicity of Hsap\HTT^GMR.Q120 expression in the eye.

Co-expression of CalpA^KK104532 can protect from the toxicity of Scer\GAL4^GMR.PF>Hsap\MAPT^Scer\UAS.cWa expression as measured by the eye area of flies.

Co-expression of CalpA^KK104532 in an Atg8a^KG07569 mutant genetic background fails to suppress the toxicity of Scer\GAL4^GMR.PF>Hsap\MAPT^Scer\UAS.cWa.

(Menzies et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v101294

P{KK104532}VIE-260B

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CalpA^KK101294

(Menzies et al., 2015)

CalpA^KK104532

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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