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General Information
Symbol
Zzzz\CAGQ48.UAS.Tag:MYC,Tag:FLAG
Species
a. artificial
Name
FlyBase ID
FBal0127243
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
P{UAS-Q48.myc/flag}
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS sequences drive expression of 48 Glutamines. This is tagged with Tag:MYC and Tag:FLAG.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 results in photoreceptor neurodegeneration in the adult eye at 7 days post-eclosion and reduced crawling ability in third instar larvae.

Expression of Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF causes eye degeneration.

Expression of Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4GMR.PF causes severe eye degeneration with loss of pigmentation in the eye and formation of black necrotic-like spots.

Expression of Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG in photoreceptors under the control of Scer\GAL4GMR.PF results in electroretinogram depolarisation amplitudes that are 55% lower than controls.

Flies expressing Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4Eaat1.PR have a shortened lifespan compared to wild type and are found to be lethargic a few days before dying.

Expression of Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4elav.PLu results in loss of 1-2 photoreceptor neurons per ommatidium and some gaps in the eye tissue.

Expression of Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG driven by Scer\GAL4elav.PLu leads to degeneration of rhabdomeres and partial lethality. Suberoylanilide hydroxamic acid (SAHA) suppresses the lethality and reduces the level of degeneration seen in the eye to wild-type levels.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of CalpAKK104532 leads to a significant reduction in the number of flies with black necrotic-like spots in the eye, in both males and females expressing Scer\GAL4GMR.PF>Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG.

Co-expression of Mmus\Cd8aScer\UAS.T:Avic\GFP does not enhance the reduction in electroretinogram depolarisation amplitude seen when Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG is expressed in photoreceptors under the control of Scer\GAL4GMR.PF.

Co-expression of Dap160Scer\UAS.cKa enhances the reduction in electroretinogram depolarisation amplitude seen when Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG is expressed in photoreceptors under the control of Scer\GAL4GMR.PF.

Co-expression of Hsap\HDSu3.Scer\UAS.T:Zzzz\CAG,T:Hsap\MYC significantly suppresses the loss of photoreceptor neurons seen in flies expressing Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG under the control of Scer\GAL4elav.PLu.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Zzzz\CAGQ48.Scer\UAS.T:Hsap\MYC,T:Zzzz\FLAG
Zzzz\CAGQ48.UAS.Tag:MYC,Tag:FLAG
Name Synonyms
Secondary FlyBase IDs
    References (10)