FlyBase Allele Report: Dmel\Pdhb[KK107865]

General Information

Symbol

Dmel\Pdhb^KK107865

Species

D. melanogaster

Name

FlyBase ID

FBal0235221

Feature type

allele

Associated gene

Dmel\Pdhb

Associated Insertion(s)

Carried in Construct

P{KK107865}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK107865}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK107865

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult brain, with Scer\GAL4^elav.PLu

(Dung et al., 2018)

axon | adult stage, with Scer\GAL4^elav.PLu

(Dung et al., 2018)

eye, with Scer\GAL4^GMR.PS

(Dung et al., 2018)

eye, with Scer\GAL4^GMR.PU

(Shimada et al., 2020)

interommatidial bristle, with Scer\GAL4^GMR.PS

(Dung et al., 2018)

larval brain | third instar larval stage, with Scer\GAL4^elav.PLu

(Dung et al., 2018)

mitochondrion | adult stage, with Scer\GAL4^elav.PLu

(Dung et al., 2018)

mitochondrion | third instar larval stage, with Scer\GAL4^elav.PLu

(Dung et al., 2018)

neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PLu

(Dung et al., 2018)

ommatidium | adult stage, with Scer\GAL4^GMR.PS

(Dung et al., 2018)

photoreceptor | adult stage, with Scer\GAL4^elav.PLu

(Dung et al., 2018)

type I bouton | third instar larval stage, with Scer\GAL4^elav.PLu

(Dung et al., 2018)

Detailed Description

Statement

Reference

The expression of Pdhb^KK107865 under the control of Scer\GAL4^GMR.PU induces a rough eye phenotype.

(Shimada et al., 2020)

Expression of Pdhb^KK107865 under the control of Scer\GAL4^GMR.PS results in rough eye phenotype associated with interommatidial bristle loss and ommatidium fusion in adult flies, the phenotype is made more severe with two copies of Pdhb^KK107865. No increase in the level of apoptosis (visualized by [*]Casp3 staining) is observed in third instar larval eye discs upon Pdhb^KK107865 expression.

Locomotor activity is perturbed by Scer\GAL4^elav.PLu-driven expression of Pdhb^KK107865 both at the larval stage (the average crawling speed is slightly but significantly reduced and the larvae frequently show aberrant crawling behavior with abnormal path patterns) and the adult stage where the climbing ability of the flies is significantly reduced already at 5 days post-eclosion and rapidly declines further with age. The lifespan of Pdhb^KK107865-expressing adult flies is also significantly shorter compared to controls.

Third instar larvae expressing Pdhb^KK107865 under Scer\GAL4^elav.PLu also display neuromuscular junction (NMJ) morphological defects: the NMJ branch length along with the number of Ib boutons is reduced, but the Ib bouton size is increased. The knock-down larvae also exhibit defects in photoreceptor axon innervation of the lamina and medulla and increased mitochondrial fragmentation in the brain. The abnormal fragmented and punctiform morphology of brain mitochondria is also seen in adult flies expressing Pdhb^KK107865 under Scer\GAL4^elav.PLu

(Dung et al., 2018)

Number of midgut cells per MARCM clone expressing Pdhb^KK107865 under the control of Scer\GAL4^Tub.PU is similar to control adults under calorie-restricted condition.

(Mattila et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Pdhb^KK107865, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by asRNA:CR43467^GD604, Scer\GAL4^GMR.PU

(Shimada et al., 2020)

Pdhb^KK107865, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible | partially by asRNA:CR43467^HMJ22886, Scer\GAL4^GMR.PU