FlyBase Allele Report: Dmel\RecQ4[14]

General Information

Symbol

Dmel\RecQ4¹⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0239075

Feature type

allele

Associated gene

Dmel\RecQ4

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Xu et al., 2009)

Mutagen

I-CreI

(Xu et al., 2009)

ends-in gene targeting

(Xu et al., 2009)

Progenitor genotype

Cytology

Description

RecQ4 gene in which the start codon ATG is replaced with CCTAGGGTCGACCCGCG, thus disrupting the translation start site and shifting the open reading frame.

(Xu et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:8,175,855..8,175,856 [-]

Inserted_sequence:

CCTAGGGTCGACCCGCG

Comment:

The start codon was replaced with CCTAGGGTCGACCCGCG, causing a frameshift. The endogenous copy of RecQ4 was replaced with the mutated copy by targeted recombination.

(Xu et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cell & dorsal mesothoracic disc

(Xu et al., 2009)

cell & larval brain

(Xu et al., 2009)

cell & larval salivary gland

(Xu et al., 2009)

chromosome

(Xu et al., 2009)

eye | somatic clone

(Xu et al., 2009)

ommatidium | somatic clone

(Xu et al., 2009)

oocyte | germline clone

(Xu et al., 2009)

Detailed Description

Statement

Reference

Nearly all RecQ4¹⁴ homozygous mutants survive for up to 8 days under normal culture conditions. However they exhibit developmental delays when compared with heterozygous siblings or wild type controls and eventually die at the early pupal stage.

Oocytes from females bearing RecQ4¹⁴ germ-line clones fail to develop beyond stage 6 of oogenesis.

At 5 days AED, the amount of DNA normalized by cell number is much lower in RecQ4¹⁴ salivary glands, compared to wild type. Mutant salivary glands also have abnormally small cells and nuclei.

At 5 days AED, RecQ4¹⁴ mutant brains incorporate much less BrdU than the wild type, indicating impaired DNA replication.

Metaphase spreads from RecQ4¹⁴ larval brains show a much higher frequency of aberrant patterns (failure of sister chromatid association and broken chromosomes) compared to wild type.

RecQ4¹⁴ show sensitivity to the mutagens paraquat and gamma irradiation, as judged by survival rates compared to controls.

Compared with wild type, more cells from RecQ4¹⁴ mutant wing imaginal discs accumulate at G2/M phase at the expense of G1/G0 and S phase cells.

RecQ4¹⁴ clones in the wing disc have fewer cells and are therefore smaller than their wild type twin spots.

RecQ4¹⁴, RecQ4^+t8.6 flies in which the RecQ4^+t8.6 rescuing transgene is specifically deleted in the eye by using Scer\FLP1^ey.PN have small rough eyes with disorganized and fewer ommatidia.

(Xu et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

When the in vivo DSB repair construct, P{Act-RFP.Rr3}, is cut (i.e. in flies with genotype Disc\RFP^{Rr3.Scer\SceI.RS.Act5C}, Scer\SCEI^Ubi-p63E.PP), RecQ4¹⁴ homozygotes show a significantly reduced survival ratio compared with heterozygous animals.

(Xu et al., 2009)

Complementation and Rescue Data

Rescued by

RecQ4¹⁴ is rescued by RecQ4^+t8.6

(Xu et al., 2009)

RecQ4¹⁴ is rescued by RecQ4^UAS.cXa/Scer\GAL4^Act.PU

(Xu et al., 2009)

RecQ4¹⁴ is rescued by Scer\GAL4^Act.PU/RecQ4^{FL.UAS.Tag:FLAG}

(Xu et al., 2009)

RecQ4¹⁴ is rescued by Scer\GAL4^Act.PU/RecQ4^{Δ868-1579.UAS.Tag:FLAG}

(Xu et al., 2009)

RecQ4¹⁴ is rescued by Scer\GAL4^Act.PU/RecQ4^{Δ1234-1579.UAS.Tag:FLAG}

(Xu et al., 2009)

RecQ4¹⁴ is rescued by Scer\GAL4^Act.PU/RecQ4^{Δ868-1207.UAS.Tag:FLAG}

(Xu et al., 2009)

Partially rescued by

RecQ4¹⁴ is partially rescued by Scer\GAL4^Act.PU/RecQ4^{Δ1234-1579.UAS.Tag:FLAG}

(Xu et al., 2009)

Not rescued by

RecQ4¹⁴ is not rescued by Scer\GAL4^Act.PU/RecQ4^{Δ868-1579.UAS.Tag:FLAG}

(Xu et al., 2009)

RecQ4¹⁴ is not rescued by Scer\GAL4^Act.PU/RecQ4^{Δ1-807.UAS.Tag:FLAG}

(Xu et al., 2009)

RecQ4¹⁴ is not rescued by Scer\GAL4^Act.PU/RecQ4^{Δ868-1207.UAS.Tag:FLAG}

(Xu et al., 2009)

RecQ4¹⁴ is not rescued by Scer\GAL4^Act.PU/RecQ4^{Δ1-1207.UAS.Tag:FLAG}

(Xu et al., 2009)

Comments

RecQ4^+t8.6 fully rescues the lethal phenotype of RecQ4¹⁴ mutants.

Expression of RecQ4^Scer\UAS.cXa using Scer\GAL4^Act fully rescues the lethal phenotype of RecQ4¹⁴ mutants.

Expression of RecQ4^{FL.Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^Act fully rescues the lethality, BrdU incorporation and gamma-irradiation sensitivity phenotypes of RecQ4¹⁴ mutants.

Expression of RecQ4^{Δ868-1579.Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^Act does not rescue the lethality or BrdU incorporation phenotypes of RecQ4¹⁴ mutants, but it does fully rescue the gamma-irradiation sensitivity phenotype.

Expression of RecQ4^{Δ1234-1579.Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^Act partially rescues (at approximately 10% efficiency) the lethality of RecQ4¹⁴ mutants, and fully rescues the BrdU incorporation and gamma-irradiation sensitivity phenotypes.

Expression of RecQ4^{Δ1-807.Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^Act fails to rescue the lethality, BrdU incorporation or gamma-irradiation sensitivity phenotypes of RecQ4¹⁴ mutants.

Expression of RecQ4^{Δ868-1207.Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^Act fails to rescue the lethality or BrdU incorporation phenotypes of RecQ4¹⁴ mutants, but this does rescue the gamma-irradiation sensitivity phenotype.

Expression of RecQ4^{Δ1-1207.Scer\UAS.T:Zzzz\FLAG} using Scer\GAL4^Act fails to rescue the lethality, BrdU incorporation or gamma-irradiation sensitivity phenotypes of RecQ4¹⁴ mutants.

(Xu et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

RecQ4¹⁴

dRecQ4¹⁴

(Xu et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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