FlyBase Allele Report: Dmel\sra[JF02557]

General Information

Symbol

Dmel\sra^JF02557

Species

D. melanogaster

Name

FlyBase ID

FBal0239258

Feature type

allele

Associated gene

Dmel\sra

Associated Insertion(s)

Carried in Construct

P{TRiP.JF02557}

Also Known As

UAS-nla-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.8.10)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.8.10)

Mutagen

in vitro construct

(Perkins et al., 2009.8.10)

Progenitor genotype

Carried in construct

P{TRiP.JF02557}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.8.10)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF02557

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease

modeled by Hsap\APP^{695.UAS.Tag:MYC}

(Shaw and Chang, 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

abnormal memory | adult stage, with Scer\GAL4^c739

(Shaw et al., 2015)

Phenotype Manifest In

synaptic vesicle | larval stage, with Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Detailed Description

Statement

Reference

Expression of sra^JF02557 under the control of Scer\GAL4^c739 results in severe short term memory defects in young (2-4 day old) adult flies in a pavlovian olfactory conditioning context.

(Shaw et al., 2015)

Expression of sra^JF02557 driven by Scer\GAL4^elav.PU does not lead to significant Syt1 aggregate formation in axons or changes in bouton number at the NMJ of third instar larvae but larvae show significant locomotor defects. Scer\GAL4^elav.PU>sra^JF02557 larval motor axons show significant decreases in vesicle transport movement and speed.

(Shaw and Chang, 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^c739, sra^JF02557 has abnormal memory | adult stage phenotype, non-enhanceable by Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739

(Shaw et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^c739, sra^JF02557 has abnormal memory | adult stage phenotype, non-suppressible by Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739

(Shaw et al., 2015)

NOT Enhancer of

Statement

Reference

sra^JF02557, Scer\GAL4^c739 is a non-enhancer of abnormal memory | adult stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739

(Shaw et al., 2015)

sra^JF02557, Scer\GAL4^elav.PU is a non-enhancer of abnormal locomotor behavior | third instar larval stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

sra^JF02557, Scer\GAL4^elav.PU is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

NOT Suppressor of

Statement

Reference

sra^JF02557, Scer\GAL4^c739 is a non-suppressor of abnormal memory | adult stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^c739

(Shaw et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

sra^JF02557, Scer\GAL4^elav.PU is an enhancer of axon | third instar larval stage phenotype of Appl^UAS.cUa, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

sra^JF02557, Scer\GAL4^elav.PU is an enhancer of synaptic vesicle | third instar larval stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

sra^JF02557, Scer\GAL4^elav.PU is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

sra^JF02557, Scer\GAL4^elav.PU is an enhancer of axon | third instar larval stage phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

sra^JF02557, Scer\GAL4^elav.PU is an enhancer of synaptic vesicle | third instar larval stage phenotype of Appl^UAS.cUa, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

sra^JF02557, Scer\GAL4^elav.PU is an enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Appl^UAS.cUa, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

NOT Enhancer of

Statement

Reference

sra^JF02557, Scer\GAL4^elav.PU is a non-enhancer of NMJ bouton | third instar larval stage | increased number phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav.PU

(Shaw and Chang, 2013)

Other

Statement

Reference

Hsap\APP^UAS.YFP, Scer\GAL4^elav.PU, sra^JF02557 has synaptic vesicle phenotype

(Shaw and Chang, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of sra^JF02557 significantly enhances aggregate formation in axons at the NMJ of Scer\GAL4^elav.PU>Appl^Scer\UAS.cUa larvae.

(Shaw and Chang, 2013)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} and sra^JF02557 (under the control of Scer\GAL4^c739) results in a similar performance index in short term memory assays as expression of either construct alone.

(Shaw et al., 2015)

Co-expression of sra^JF02557 significantly enhances aggregate formation in axons at the NMJ (but does not enhance locomotor defects) of Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} larvae; Syt1 anterograde and retrograde transport and speed is also reduced in larval motor axons. Co-expression of sra^JF02557 does not enhance bouton number (synapse proliferation) or satellite bouton number at the NMJ in Scer\GAL4^elav.PU>Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} larvae.

(Shaw and Chang, 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27260

y¹ v¹; P{TRiP.JF02557}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

sra^JF02557

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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