FlyBase Allele Report: Dmel\yki[UAS.Tag:MYC]

General Information

Symbol

Dmel\yki^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0239892

Feature type

allele

Associated gene

Dmel\yki

Associated Insertion(s)

Carried in Construct

P{UAS-yki.Myc}

Also Known As

UAS-Yki, UAS-Myc-Yki

Key Links

Transgenic product class

wild_type

(Zhang et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Zhang et al., 2008)

Mutagen

in vitro construct

(Zhang et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-yki.Myc}

Cytology

Description

UASt regulatory sequences drive expression of yki which is tagged at the N-terminal end with six copies of Tag:MYC.

(Zhang et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

yki

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage, with Scer\GAL4^GMR.PF

(Cho et al., 2018)

increased occurrence of cell division | larval stage, with Scer\GAL4^GMR.PU

(Guo et al., 2013)

visible, with Scer\GAL4^GMR.PF

(Ren et al., 2010)

visible, with Scer\GAL4^GMR.PU

(Guo et al., 2013)

visible | adult stage, with Scer\GAL4^GMR.PF

(Cho et al., 2018)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Cho et al., 2018, Ren et al., 2010)

eye, with Scer\GAL4^GMR.PU

(Guo et al., 2013)

eye disc, with Scer\GAL4^GMR.PU

(Guo et al., 2013)

Detailed Description

Statement

Reference

Expression of yki^UAS.Tag:MYC under the control of Scer\GAL4^GMR.PF leads to an increase in eye size when compared to controls.

(Cho et al., 2018)

Expression of yki^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU results in increased DNA replication in the eye disc (assayed by incorporation of BrdU) and overgrowth of the eye.

(Guo et al., 2013)

Expression of yki^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF results in an enlarged eye phenotype.

(Ren et al., 2010)

Expression of yki^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF induces little, if any, overgrowth of adult eyes.

(Zhang et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible | adult stage phenotype, enhanceable by Prp4k^KK100483, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has abnormal size | adult stage phenotype, enhanceable by Prp4k^HMC03788, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible | adult stage phenotype, enhanceable by Prp4k^HMC03788, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has abnormal size | adult stage phenotype, enhanceable by Prp4k^GD12108, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible | adult stage phenotype, enhanceable by Prp4k^GD12108, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has abnormal size | adult stage phenotype, enhanceable by Scer\FLP1^ey.PU, Prp4k^EY11156/Prp4k^EY11156

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible | adult stage phenotype, enhanceable by Scer\FLP1^ey.PU, Prp4k^EY11156/Prp4k^EY11156

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has abnormal size | adult stage phenotype, enhanceable by Prp4k^KK100483, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible phenotype, enhanceable by 14-3-3ζ^RNAi.UAS.cUa, Scer\GAL4^GMR.PF

(Ren et al., 2010)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible phenotype, enhanceable by 14-3-3ε^RNAi.UAS, Scer\GAL4^GMR.PF

(Ren et al., 2010)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible phenotype, enhanceable by 14-3-3ε^j2B10/14-3-3epsilon[+]

(Ren et al., 2010)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible phenotype, enhanceable by 14-3-3ζ^12BL/14-3-3zeta[+]

(Ren et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has abnormal size | adult stage phenotype, suppressible by Prp4k^UAS.GFP, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible | adult stage phenotype, suppressible by Prp4k^UAS.GFP, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PU, sd^UAS.Tag:HA, yki^UAS.Tag:MYC has visible phenotype, suppressible by Tgi^UAS.Tag:FLAG/Scer\GAL4^GMR.PU

(Guo et al., 2013)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible phenotype, suppressible by 14-3-3ε^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Ren et al., 2010)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible phenotype, suppressible by 14-3-3ζ^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Ren et al., 2010)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible phenotype, suppressible by 14-3-3ζ^UAS.Tag:HA/14-3-3ε^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Ren et al., 2010)

14-3-3ε^RNAi.UAS, Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible phenotype, suppressible by 14-3-3ζ^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Ren et al., 2010)

14-3-3ζ^RNAi.UAS.cUa, Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible phenotype, suppressible by 14-3-3ε^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Ren et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has abnormal size | adult stage phenotype, non-suppressible by Prp4k^KR.UAS.GFP, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has visible | adult stage phenotype, non-suppressible by Prp4k^KR.UAS.GFP, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, enhanceable by Prp4k^HMC03788, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, enhanceable by Prp4k^GD12108, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, enhanceable by Scer\FLP1^ey.PU, Prp4k^EY11156/Prp4k^EY11156

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, enhanceable by Prp4k^KK100483, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, enhanceable by 14-3-3ζ^RNAi.UAS.cUa, Scer\GAL4^GMR.PF

(Ren et al., 2010)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, enhanceable by 14-3-3ε^RNAi.UAS, Scer\GAL4^GMR.PF

(Ren et al., 2010)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, enhanceable by 14-3-3ε^j2B10/14-3-3epsilon[+]

(Ren et al., 2010)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, enhanceable by 14-3-3ζ^12BL/14-3-3zeta[+]

(Ren et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, suppressible by Prp4k^UAS.GFP, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Scer\GAL4^GMR.PU, sd^UAS.Tag:HA, yki^UAS.Tag:MYC has eye phenotype, suppressible by Tgi^UAS.Tag:FLAG/Scer\GAL4^GMR.PU

(Guo et al., 2013)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, suppressible by 14-3-3ζ^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Ren et al., 2010)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, suppressible by 14-3-3ζ^UAS.Tag:HA/14-3-3ε^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Ren et al., 2010)

14-3-3ε^RNAi.UAS, Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, suppressible by 14-3-3ζ^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Ren et al., 2010)

14-3-3ζ^RNAi.UAS.cUa, Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, suppressible by 14-3-3ε^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Ren et al., 2010)

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, suppressible by 14-3-3ε^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Ren et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, yki^UAS.Tag:MYC has eye phenotype, non-suppressible by Prp4k^KR.UAS.GFP, Scer\GAL4^GMR.PF

(Cho et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Eye specific clones (generated using ey-FLP) of Prp4k^EY11156 enhance the increased eye size phenotype of yki^UAS.Tag:MYC expression under the control of Scer\GAL4^GMR.PF when compared to control clones.

(Cho et al., 2018)

Co-expression of Tgi^{Scer\UAS.T:Zzzz\FLAG} suppresses the increased DNA replication in the eye disc (assayed by incorporation of BrdU) and overgrowth of the eye seen in animals expressing yki^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU.

Co-expression of Tgi^{Scer\UAS.T:Zzzz\FLAG} suppresses the eye overgrowth seen in flies expressing sd^{Scer\UAS.T:Ivir\HA1} and yki^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PU.

(Guo et al., 2013)

Co-expression of either 14-3-3ζ^VDRC.cUa or 14-3-3ε^{dsRNA.Scer\UAS} enhances the eye overgrowth phenotype caused by expression of yki^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF.

The ability of 14-3-3ε^{dsRNA.Scer\UAS} to enhance the eye overgrowth phenotype caused by expression of yki^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF is significantly suppressed by co-expression of 14-3-3ζ^{Scer\UAS.T:Ivir\HA1}.

The ability of 14-3-3ζ^VDRC.cUa to enhance the eye overgrowth phenotype caused by expression of yki^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF is significantly suppressed by co-expression of 14-3-3ε^{Scer\UAS.T:Ivir\HA1}.

The eye overgrowth phenotype caused by expression of yki^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF is enhanced by 14-3-3ε^j2B10/+ and by 14-3-3ζ^12BL/+.

Co-expression of either 14-3-3ε^{Scer\UAS.T:Ivir\HA1}, 14-3-3ζ^{Scer\UAS.T:Ivir\HA1} or both 14-3-3ε^{Scer\UAS.T:Ivir\HA1} and 14-3-3ζ^{Scer\UAS.T:Ivir\HA1} together suppresses the eye overgrowth phenotype caused by expression of yki^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF.

(Ren et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

yki^{Scer\UAS.T:Hsap\MYC}

yki^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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