FlyBase Allele Report: Dmel\alien[HM05119]

General Information

Symbol

Dmel\alien^HM05119

Species

D. melanogaster

Name

FlyBase ID

FBal0240248

Feature type

allele

Associated gene

Dmel\alien

Associated Insertion(s)

Carried in Construct

P{TRiP.HM05119}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009)

Mutagen

in vitro construct

(Perkins et al., 2009)

Progenitor genotype

Carried in construct

P{TRiP.HM05119}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HM05119

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response | larval stage, with Scer\GAL4^ppl.PP

(Zhao et al., 2022)

Phenotype Manifest In

cyst cell of testis, with Scer\GAL4^A3.eya

(Qian et al., 2015)

cyst cell of testis, with Scer\GAL4^c784

(Qian et al., 2015)

cyst cell of testis, with Scer\GAL4^NP1624

(Qian et al., 2015)

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^ppl.PP

(Zhao et al., 2022)

lipid droplet | third instar larval stage, with Scer\GAL4^ppl.PP

(Zhao et al., 2022)

testis, with Scer\GAL4^A3.eya

(Qian et al., 2015)

testis, with Scer\GAL4^c784

(Qian et al., 2015)

testis, with Scer\GAL4^NP1624

(Qian et al., 2015)

Detailed Description

Statement

Reference

The expression of alien^HM05119 under the control of Scer\GAL4^ppl.PP leads to fewer peripheral lipid droplets in the third instar larval fat body. Larvae show lower survival to starvation, compared to controls.

(Zhao et al., 2022)

Expression of alien^HM05119 under the control of any of the cyst cell drivers Scer\GAL4^tj-NP1624, Scer\GAL4^A3.eya or Scer\GAL4^c784 (limited to a week from eclosion onwards using Scer\GAL80^ts.αTub84B) results in a 100% penetrant testes phenotype that is never observed in controls. The apical region of the testis contain large areas containing gonialblasts and spermatogonia, and the rest of the testis is filled with cell debris. No spermatocytes are seen and, as in wild type, germline stem cells are only observed next to the hub. The net-like somatic support cell cytoplasmic extensions usually seen surrounding the germline cells are not observed (only very few and short cytoplasmic extensions are seen), but the support cells themselves are readily detectable. Loss of cytoplasmic extensions is seen very quickly after the onset of alien^HM05119 expression, and there is no evidence of cell death. The ability of the somatic cells to divide by mitosis is unaffected.

(Qian et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ppl.PP, alien^HM05119, mdy^UAS.cHa has embryonic/larval fat body | third instar larval stage phenotype, suppressible by Rdh1^TH01566.N, Scer\GAL4^ppl.PP

(Zhao et al., 2022)

Scer\GAL4^ppl.PP, alien^HM05119, mdy^UAS.cHa has lipid droplet | third instar larval stage phenotype, suppressible by Rdh1^TH01566.N, Scer\GAL4^ppl.PP

(Zhao et al., 2022)

Other

Statement

Reference

Scer\GAL4^ppl.PP, alien^HM05119, mdy^UAS.cHa has embryonic/larval fat body | third instar larval stage phenotype

(Zhao et al., 2022)

Scer\GAL4^ppl.PP, alien^HM05119, mdy^UAS.cHa has lipid droplet | third instar larval stage phenotype

(Zhao et al., 2022)

Rdh1^TH01566.N, Scer\GAL4^ppl.PP, alien^HM05119 has embryonic/larval fat body | third instar larval stage phenotype

(Zhao et al., 2022)

Rdh1^TH01566.N, Scer\GAL4^ppl.PP, alien^HM05119 has lipid droplet | third instar larval stage phenotype

(Zhao et al., 2022)

Additional Comments

Genetic Interactions

Statement

Reference

The double expression of mdy^UAS.cHa with alien^HM05119 under the control of Scer\GAL4^ppl.PP leads to enlarged lipid droplets in the third instar larval fat body; there are fewer peripheral lipid droplets.

The double expression of Rdh1^TH01566.N with alien^HM05119 under the control of Scer\GAL4^ppl.PP leads to small lipid droplets in the fat body of third instar larvae fed on a high-fat diet.

(Zhao et al., 2022)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

28908

y¹ v¹; P{TRiP.HM05119}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

alien^HM05119

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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