FB2026_02 , released June 18, 2026
Allele: Dmel\vari48EP
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General Information
Symbol
Dmel\vari48EP
Species
D. melanogaster
Name
FlyBase ID
FBal0240434
Feature type
allele
Associated gene
Dmel\vari
Associated Insertion(s)
P{?EP}vari48EP
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Moyer and Jacobs, 2008)
Mutagen
P-element activity
(Moyer and Jacobs, 2008)
Progenitor genotype
P{EP}variGE13049
(Moyer and Jacobs, 2008)
Associated Insertion(s)
P{?EP}vari48EP
Caused by aberration
Df(2L)vari48EP
Cytology
Description

Imprecise excision of the progenitor insertion, resulting in a 4717bp deletion (coordinates 2L: 20792456...20797173) that removes part of the vari transcription unit. 416bp of the original insertions remains. The deletion also removes the 3'UTR and the 3 C-terminal amino acids of Pomp.

(Moyer and Jacobs, 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2L:20,788,525..20,793,242 [-]
Comment:

4717bp deletion resulting from the imprecise excision of P{EP}variGE13049. Removes most of vari coding sequence and the last three amino acids of Pomp. Position of deletion on reference sequence inferred by FlyBase curator.

(Moyer and Jacobs, 2008)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      No significant increase in defasciculation defects in intersegmental nerve b motor axons is observed in vari48EP heterozygous embryos compared to controls.

      vari327/vari48EP transheterozygous embryos display increased frequency of defasciculation defects in intersegmental nerve b motor axons.

      (Jeong et al., 2017)

      The stage 15 embryonic hindguts of vari48EP homozygotes are of normal length but present a lower angle of curvature, as compared to controls.

      (Wells et al., 2013)

      Late embryonic lethal.

      Homozygous embryos show loss of integrity of the transepithelial barrier; dextran dye is detected within the tracheal lumen of mutant embryos within 30 minutes of injection. The lumens of stage 16 trachea can be significantly dilated in mutant embryos compared to wild type, with large dilations being present along the dorsal trunk, but the structure of the lumenal cuticle and taenideal ridges is unaffected. Some lateral branches appear disconnected from the dorsal trunk.

      The ultrastructure of the lateral membranes and septate junctions of epithelial cells in the hindgut is similar in mutant and wild-type embryos.

      vari48EP/vari03953b embryos have large dilations along the dorsal trunk.

      Homozygous clones are not recovered in the adult eye, although the eyes contain fused ommatidia, missing bristles and duplicated bristles.

      (Moyer and Jacobs, 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      vari[+]/vari48EP is an enhancer of abnormal neuroanatomy | embryonic stage phenotype of Sema1ak13702

      (Jeong et al., 2017)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      vari[+]/vari48EP is an enhancer of larval intersegmental nerve branch ISNb of A1-7 | embryonic stage phenotype of Sema1ak13702

      (Jeong et al., 2017)

      vari[+]/vari48EP is an enhancer of axon | embryonic stage phenotype of Sema1ak13702

      (Jeong et al., 2017)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The moderate guidance defects observed in intersegmental nerve b motor axons of Sema1ak13702 heterozygous embryos are exacerbated by combination with a single copy of vari48EP.

      (Jeong et al., 2017)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      variGE13049

      (Moyer and Jacobs, 2008)
      Partially rescued by

      vari48EP is partially rescued by variUAS.cMa/Scer\GAL4da.G32

      (Moyer and Jacobs, 2008)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      vari48EP
      (Jeong et al., 2017, Wells et al., 2013, Moyer and Jacobs, 2008)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)