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General Information
Symbol
Dmel\RhoGAP18BRC.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0240549
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the RhoGAP18B-RC transcript (that overlaps with RhoGAP18B-RD).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of RhoGAP18BRC.Scer\UAS under the control of Scer\GAL4whir3 has no effect.

Adult overexpression of RhoGAP18BRC.Scer\UAS in wild-type flies (Scer\GAL4whir3/+, Scer\GAL80ts.αTub84B; Avic\GFPScer\UAS) does not change ethanol-induced hyperactivity.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of RhoGAP18BRC.Scer\UAS under the control of Scer\GAL4whir3 in RhoGAP18B3 mutant males results in nearly normal ethanol sedation. This behavioural rescue is manifested as a reduction in the time required for 50% of the flies to reach sedation. In addition, RhoGAP18BRC.Scer\UAS expression also increases the viability of the RhoGAP18B3 mutant from 31% to 83%.

Expression of RhoGAP18BRC.Scer\UAS under the control of Scer\GAL4whir1 in RhoGAP18B1 mutants ameliorates the ethanol-resistance phenotype of RhoGAP18B1. The phenotype rescue is, however, incomplete, probably due to the fact that expression of RhoGAP18BRC.Scer\UAS under the control of Scer\GAL4whir1 is low.

Expression of RhoGAP18BRC.Scer\UAS under the control of Scer\GAL4whir3, using Scer\GAL80ts.αTub84B to block expression of Scer\GAL4whir3 at the permissive temperature (16[o]C), except for three days in adulthood, where the restrictive (25[o]C or 29[o]C) temperature is used to allow expression of Scer\GAL4whir3 to drive RhoGAP18BRC.Scer\UAS reveals a complete rescue of the RhoGAP18B3 ethanol-induced sedation phenotype. The reciprocal experiment, where the flies are raised at 25[o]C and then placed at 16[o]C for 3 days prior to behavioural testing fails to rescue the RhoGAP18B3 ethanol-induced sedation phenotype and in fact worsens the phenotype compared to control flies.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
RhoGAP18BRC.Scer\UAS
RhoGAP18BRC.UAS
Name Synonyms
Secondary FlyBase IDs
    References (2)