FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\RhoGAP18BRC.UAS
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General Information
Symbol
Dmel\RhoGAP18BRC.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0240549
Feature type
allele
Associated gene
Dmel\RhoGAP18B
Associated Insertion(s)
Carried in Construct
P{UAS-RhoGAP18B.RC}
Key Links
Transgenic product class
wild_type
(Rothenfluh et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Rothenfluh et al., 2006)
Mutagen
in vitro construct
(Rothenfluh et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-RhoGAP18B.RC}
Cytology
Description

UASt regulatory sequences drive expression of the RhoGAP18B-RC transcript (that overlaps with RhoGAP18B-RD).

(Rothenfluh et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
RhoGAP18B
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  alcohol use disorder
      CEA
      (Rothenfluh et al., 2006)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of RhoGAP18BRC.Scer\UAS under the control of Scer\GAL4whir3 has no effect.

      Adult overexpression of RhoGAP18BRC.Scer\UAS in wild-type flies (Scer\GAL4whir3/+, Scer\GAL80ts.αTub84B; Avic\GFPScer\UAS) does not change ethanol-induced hyperactivity.

      (Rothenfluh et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4NP1514/RhoGAP18BRC.UAS rescues RhoGAP18BNP1514

      (Gonzalez et al., 2018)

      RhoGAP18BRC.UAS/Scer\GAL4RhoGAP18B-1 rescues RhoGAP18B1

      (Rothenfluh et al., 2006)

      Scer\GAL4NP1514/RhoGAP18BRC.UAS rescues RhoGAP18BNP1514

      (Rothenfluh et al., 2006)
      Fails to rescue

      Scer\GAL4NP1514, RhoGAP18BRC.UAS, Scer\GAL80ts.αTub84B fails to rescue RhoGAP18BNP1514

      (Rothenfluh et al., 2006)
      Comments

      Expression of RhoGAP18BRC.Scer\UAS under the control of Scer\GAL4whir3 in RhoGAP18B3 mutant males results in nearly normal ethanol sedation. This behavioural rescue is manifested as a reduction in the time required for 50% of the flies to reach sedation. In addition, RhoGAP18BRC.Scer\UAS expression also increases the viability of the RhoGAP18B3 mutant from 31% to 83%.

      Expression of RhoGAP18BRC.Scer\UAS under the control of Scer\GAL4whir1 in RhoGAP18B1 mutants ameliorates the ethanol-resistance phenotype of RhoGAP18B1. The phenotype rescue is, however, incomplete, probably due to the fact that expression of RhoGAP18BRC.Scer\UAS under the control of Scer\GAL4whir1 is low.

      Expression of RhoGAP18BRC.Scer\UAS under the control of Scer\GAL4whir3, using Scer\GAL80ts.αTub84B to block expression of Scer\GAL4whir3 at the permissive temperature (16[o]C), except for three days in adulthood, where the restrictive (25[o]C or 29[o]C) temperature is used to allow expression of Scer\GAL4whir3 to drive RhoGAP18BRC.Scer\UAS reveals a complete rescue of the RhoGAP18B3 ethanol-induced sedation phenotype. The reciprocal experiment, where the flies are raised at 25[o]C and then placed at 16[o]C for 3 days prior to behavioural testing fails to rescue the RhoGAP18B3 ethanol-induced sedation phenotype and in fact worsens the phenotype compared to control flies.

      (Rothenfluh et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      RhoGAP18BRC.Scer\UAS
      RhoGAP18BRC.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (2)