FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\RhoGAP18BNP1514
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General Information
Symbol
Dmel\RhoGAP18BNP1514
Species
D. melanogaster
Name
FlyBase ID
FBal0281070
Feature type
allele
Associated gene
Dmel\RhoGAP18B
Associated Insertion(s)
P{GawB}RhoGAP18BNP1514
Carried in Construct
Also Known As
whir3, whir3-GAL4
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Rothenfluh et al., 2006)
Progenitor genotype
Associated Insertion(s)
P{GawB}RhoGAP18BNP1514
Cytology
Description

A P-element is inserted within an intron inside RhoGAP18B.

(Rothenfluh et al., 2006)
Allele components
Component
Use(s)
Inserted element
P{GawB}
Encoded product / tool
GAL4
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  alcohol use disorder
      CEA
      (Rothenfluh et al., 2006)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      RhoGAP18B3 mutants exhibit resistance to ethanol-induced sedation measured with either a locomotor tracking system or a loss-of-righting (LOR) test. Ethanol absorption is normal in RhoGAP18B3 flies, which also perform normally in various behavioural assays, including those measuring sensitivity to CO[[2]]-induced sedation, nicotine toxicity, spontaneous locomotion, circadian rhythms, and negative geotaxis. RhoGAP18B3 flies also show increased resistance to the acute effects of nicotine and cocaine.

      RhoGAP18B3 mutants exhibit reduced viability (31% viability).

      RhoGAP18B3 mutant flies exhibit significantly reduced loss-of-righting (LOR-refers to the inability of flies to regain upright posture upon mechanical stimulation) compared to control flies.

      The brain structures of RhoGAP18B3 mutant flies do not seem morphologically different from wild-type.

      (Rothenfluh et al., 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Statement
      Reference

      RhoGAP18BNP1514 has partially lethal - majority die phenotype, suppressible by Arfip12/Arfip[+]

      (Gonzalez et al., 2018)

      RhoGAP18BNP1514 has partially lethal - majority die phenotype, suppressible by Arf51F[+]/Arf6NP5226

      (Gonzalez et al., 2018)

      RhoGAP18BNP1514 has partially lethal - majority die phenotype, suppressible by Efa6[+]/Efa6c00953

      (Gonzalez et al., 2018)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      RhoGAP18BNP1514 is rescued by Scer\GAL4NP1514/RhoGAP18BRC.UAS

      (Gonzalez et al., 2018)

      RhoGAP18BNP1514 is rescued by Scer\GAL4NP1514/RhoGAP18BRC.UAS

      (Rothenfluh et al., 2006)
      Not rescued by

      RhoGAP18BNP1514 is not rescued by Scer\GAL4NP1514/RhoGAP18BRC.UAS/Scer\GAL80ts.αTub84B

      (Rothenfluh et al., 2006)
      Comments

      Expression of RhoGAP18BRC.Scer\UAS under the control of Scer\GAL4whir3 in RhoGAP18B3 mutant males results in nearly normal ethanol sedation. This behavioural rescue is manifested as a reduction in the time required for 50% of the flies to reach sedation. In addition, RhoGAP18BRC.Scer\UAS expression also increases the viability of the RhoGAP18B3 mutant from 31% to 83%.

      (Rothenfluh et al., 2006)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Based on resistance to enthanol-induced sedation, the following RhoGAP18B alleles can be ranked from strongest to weakest as follows: RhoGAP18B3 > RhoGAP18B1 = RhoGAP18BΔRC.

      (Rothenfluh et al., 2006)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      RhoGAP18B3
      RhoGAP18BNP1514
      whir3
      (Gonzalez et al., 2018, Peru Y Colón de Portugal et al., 2012, Rothenfluh et al., 2006)
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0228863
      • FBal0240544
      References (4)