FlyBase Allele Report: Dmel\Pld[UAS.cLa]

General Information

Symbol

Dmel\Pld^UAS.cLa

Species

D. melanogaster

Name

FlyBase ID

FBal0240724

Feature type

allele

Associated gene

Dmel\Pld

Associated Insertion(s)

Carried in Construct

P{UAS-Pld.L}

Also Known As

P{UAS-Pld}

Key Links

Transgenic product class

cDNA

(LaLonde et al., 2006)

wild_type

(LaLonde et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(LaLonde et al., 2006)

wild_type

(LaLonde et al., 2006)

Mutagen

in vitro construct

(Kwon and Montell, 2006, LaLonde et al., 2006, LaLonde et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Pld.L}

Cytology

Description

UASt regulatory sequences drive expression of a 3.9 kb Xba I-BamH I fragment containing the the Pld coding region from cDNA clone GH07346.

(LaLonde et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Pld

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with Scer\GAL4^nanos.PG

(LaLonde et al., 2006)

some die during embryonic stage, with Scer\GAL4^nanos.PG

(LaLonde et al., 2006)

viable, with Scer\GAL4^nanos.PG

(LaLonde et al., 2006)

Phenotype Manifest In

eye, with Scer\GAL4^unspecified

(Kwon and Montell, 2006)

eye photoreceptor cell, with Scer\GAL4^ninaE.PD

(LaLonde et al., 2005)

ommatidium, with Scer\GAL4^ninaE.PD

(LaLonde et al., 2005)

retina, with Scer\GAL4^ninaE.PD

(LaLonde et al., 2005)

rhabdomere, with Scer\GAL4^ninaE.PD

(LaLonde et al., 2005)

rhabdomere, with Scer\GAL4^unspecified

(Kwon and Montell, 2006)

Detailed Description

Statement

Reference

One-day old flies in which Pld^Scer\UAS.La is expressed under the control of Scer\GAL4^unspecified display early stages of degeneration and are typically missing one rhabdomere per ommatidium. Two-days post-eclosion, these flies display clear retinal degeneration, with the numbers and sizes of the rhabdomeres greatly reduced. The degeneration gradually progresses until nearly all of the rhabdomeres disappear in seven-day old Pld^Scer\UAS.La Scer\GAL4^unspecified flies.

(Kwon and Montell, 2006)

Expression of any of three different Pld^Scer\UAS.cLa transgenic lines under the simultaneous control of both P{GAL4-nos.NGT}40 and P{GAL4-nos.NGT}A results in embryonic lethality (averaging 30.4%).

Expression of any of three different Pld^Scer\UAS.cLa transgenic lines under the control of P{GAL4-nos.NGT}11 results in viable flies.

(LaLonde et al., 2006)

Expression of Pld^Scer\UAS.cLa under the control of Scer\GAL4^ninaE.PD results in progressive retinal degeneration when flies are raised under a 12 hour light/12 hour dark cycle: after 21 days, there is a clear loss of photoreceptor cells and increased intracellular vacuoles relative to controls. Maintaining these flies in the dark substantially decreases the severity of the phenotype.

Flies expressing Pld^Scer\UAS.cLa under the control of Scer\GAL4^ninaE.PD and raised under continuous light show some retinal degeneration after 1 day, and show severe retinal degeneration after 3 days - reduced rhabdomeres and degenerating photoreceptor cell bodies are visible.

(LaLonde et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^ninaE.PD/Pld^UAS.cLa is a suppressor of abnormal neurophysiology phenotype of rdgB⁹

(LaLonde et al., 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ninaE.PD/Pld^UAS.cLa is a non-suppressor of abnormal neurophysiology phenotype of norpA^EE5

(LaLonde et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Pld^UAS.cLa, Scer\GAL4^unspecified has rhabdomere phenotype, suppressible | partially by laza¹, Scer\GAL4^unspecified

(Kwon and Montell, 2006)

Pld^UAS.cLa, Scer\GAL4^unspecified has eye phenotype, suppressible | partially by laza¹, Scer\GAL4^unspecified

(Kwon and Montell, 2006)

Pld^UAS.cLa, Scer\GAL4^ninaE.PD has retina phenotype, suppressible by trp¹

(LaLonde et al., 2005)

Pld^UAS.cLa, Scer\GAL4^ninaE.PD has retina phenotype, suppressible by rdgB⁹

(LaLonde et al., 2005)

Suppressor of

Statement

Reference

Scer\GAL4^ninaE.PD/Pld^UAS.cLa is a suppressor of retina phenotype of norpA^EE5

(LaLonde et al., 2005)

Scer\GAL4^ninaE.PD/Pld^UAS.cLa is a suppressor of retina phenotype of rdgB⁹

(LaLonde et al., 2005)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ninaE.PD/Pld^UAS.cLa is a non-suppressor of retina phenotype of norpA^EE5

(LaLonde et al., 2005)

Scer\GAL4^ninaE.PD/Pld^UAS.cLa is a non-suppressor of retina phenotype of rdgB⁹

(LaLonde et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Retinal degeneration is significantly reduced in 2-day-old Pld^Scer\UAS.La (Scer\GAL4^unspecified) ; laza¹ flies relative to Pld^Scer\UAS.La (Scer\GAL4^unspecified flies. Suppression is detected in 3-day-old flies, however, by 7 days post-eclosion, the differences between Pld^Scer\UAS.La (Scer\GAL4^unspecified and Pld^Scer\UAS.La (Scer\GAL4^unspecified ; laza¹ flies are not significant.

(Kwon and Montell, 2006)

The absence of light-stimulated change in the membrane potential of retinal photoreceptors in norpA⁷ flies is not rescued by expressing Pld^Scer\UAS.cLa under the control of Scer\GAL4^ninaE.PD.

Expression of Pld^Scer\UAS.cLa under the control of Scer\GAL4^ninaE.PD partially suppresses the norpA⁷ retinal degeneration phenotype.

The retinal degeneration phenotype observed upon expression of Pld^Scer\UAS.cLa under the control of Scer\GAL4^ninaE.PD when flies are raised under continuous light for 1 day is suppressed in a trp¹ mutant background.

Expression of Pld^Scer\UAS.cLa under the control of Scer\GAL4^ninaE.PD suppresses the defective light response phenotype of 1 day-old rdgB⁹ mutants.

The retinal degeneration phenotype observed upon expression of Pld^Scer\UAS.cLa under the control of Scer\GAL4^ninaE.PD when flies are raised under continuous light for 1 or 3 days is suppressed in a rdgB⁹ mutant background. Conversely, expression of Pld^Scer\UAS.cLa under the control of Scer\GAL4^ninaE.PD does not prevent or slow the rate of retinal degeneration seen in rdgB⁹ flies.

(LaLonde et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Pld^Scer\UAS.La

Pld^Scer\UAS.cLa

Pld^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of LaLonde

Secondary FlyBase IDs

FBal0194321
FBal0196336

References (4)

Report Sections

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