FB2026_02 , released June 18, 2026
Allele: Dmel\Orc6W228A.K229A.Orc6.UAS.GFP
Open Close
General Information
Symbol
Dmel\Orc6W228A.K229A.Orc6.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0241172
Feature type
allele
Associated gene
Dmel\Orc6
Associated Insertion(s)
Carried in Construct
P{Orc6-UAS-Orc6.N-GFP.W228A.K229A}
Key Links
Transgenic product class
missense_variant
(Balasov et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Balasov et al., 2009)
Mutagen
in vitro construct
(Balasov et al., 2009)
Progenitor genotype
Carried in construct
P{Orc6-UAS-Orc6.N-GFP.W228A.K229A}
Cytology
Description

The native Orc6 promoter and UAS regulatory sequences are fused upstream of a mutated form of Orc6 (contains the amino acid replacements W228A, K229A), which is tagged at the N-terminal end with GFP.

(Balasov et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
Orc6
UAS
Encoded product / tool
Orc6
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      Scer\GAL4Tub.PU/Orc6W228A.K229A.Orc6.UAS.GFP fails to rescue Orc635

      (Balasov et al., 2015)

      Orc6W228A.K229A.Orc6.UAS.GFP fails to rescue Orc635

      (Balasov et al., 2015)

      Orc6W228A.K229A.Orc6.UAS.GFP fails to rescue Orc635

      (Balasov et al., 2009)
      Comments

      Expression of Orc6W228A.K229A.Orc6.Scer\UAS.T:Avic\GFP under the control of either its native promoter or the Scer\GAL4tub.PU driver does not rescue either the third instar stage lethality or the aberrant karyotype of Orc635 mutant larvae.

      (Balasov et al., 2015)

      Expression of Orc6W228A.K229A.T:Avic\GFP results in the release of Orc635 mutant cells from G1 arrest, as shown by the ratio of cells containing either 1 or 2 centrosomes being indistinguishable from cells obtained from heterozygous siblings.

      Chromosomes from neuroblasts isolated from Orc635 mutants carrying Orc6W228A.K229A.T:Avic\GFP are able to incorporate BrdU, and therefore are able to replicate DNA. Brains observed in these larvae are significantly reduced in size compared with heterozygous or wild-type larval brains, suggesting severe defects in cell proliferation and larval brain development.

      Orc635 mutant neuroblasts carrying Orc6W228A.K229A.T:Avic\GFP accumulate at mitosis. The number of mitotic neuroblasts derived from these transgenic flies increases approximately 8-fold compared with Orc635 mutant cells.

      A large number of metaphase-like figures are observed in Orc635 mutant cells expressing Orc6W228A.K229A.T:Avic\GFP. Cells in other stages are also found (e.g. anaphase-like figures are found, containing broken chromosomes), some polyploid with multiple centrosomes, while others exhibit multiple centrosomes but don't have any DNA present.

      (Balasov et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Orc6W228A.K229A.Orc6.Scer\UAS.T:Avic\GFP
      Orc6W228A.K229A.Orc6.UAS.GFP
      Orc6W228A.K229A.T:Avic\GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)