FlyBase Allele Report: Dmel\Orc6[35]

General Information

Symbol

Dmel\Orc6³⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0241167

Feature type

allele

Associated gene

Dmel\Orc6

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Balasov et al., 2009)

Mutagen

Delta2-3 transposase

(Balasov et al., 2009)

Progenitor genotype

Caused by aberration

Df(2R)Orc6³⁵

Cytology

Description

Imprecise excision of P{EPgy2}EY04071 (mapped 71bp upstream of the Orc6 start codon) results in deletion of the whole Orc6 coding region and some of CG1667.

(Balasov et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Meier-Gorlin syndrome 3

CEA

(Balasov et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Meier-Gorlin syndrome 3

is ameliorated by Orc6^Orc6.UAS.GFP

(Balasov et al., 2020)

is ameliorated by Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}

(Balasov et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Balasov et al., 2009)

abnormal mitotic cell cycle | larval stage

(Balasov et al., 2020)

abnormal neuroanatomy | embryonic stage

(Balasov et al., 2009)

abnormal neuroanatomy | larval stage

(Balasov et al., 2020, Balasov et al., 2009)

abnormal neuroanatomy | larval stage, with Orc6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

decreased occurrence of cell division | larval stage

(Balasov et al., 2009)

decreased size | adult stage, with Orc6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

decreased size | larval stage

(Balasov et al., 2020)

decreased size | larval stage, with Orc6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

flightless, with Orc6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

lethal

(Balasov et al., 2020, Balasov et al., 2015)

lethal - all die before end of larval stage

(Balasov et al., 2009)

some die during third instar larval stage

(Balasov et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

In Orc6³⁵ homozygotes, brains are underdeveloped; larval neuroblasts show prevalent karyotype defects, namely chromosomes with missing parts, aberrantly condensed, and fragmented.

The larval brain of Orc6³⁵/Orc6³⁵, Orc6^{K23E.Orc6.UAS.GFP}/Orc6^{K23E.Orc6.UAS.GFP} is underdeveloped. The surviving adult frequently exhibit missing/defective scutellar bristles and a small and misshaped eye.

(Balasov et al., 2020)

Orc6³⁵ mutants are lethal and die during third instar larval stage; on the cellular level they display chromosomal abnormalities in larval brain cells.

(Balasov et al., 2015)

The optic lobes of Orc6³⁵ mutant brains are significantly smaller in size compared with the wild-type or heterozygous animals. BrdU incorporation is also severely reduced in homozygous Orc6³⁵ mutant larval brains compared with the large levels of BrdU incorporation in heterozygous brains, demonstrating the lack of DNA replication. No imaginal discs are detected in late third instar larvae of Orc6³⁵ mutant animals.

Orc6³⁵ mutant salivary glands appear morphologically normal, but are reduced in size. BrdU incorporation in salivary glands is close to the levels of the wild-type or heterozygous animals, suggesting that DNA replication is not affected by the deletion of Orc6.

Orc6³⁵ mutant cells are delayed in cell cycle progression. The ratio of G1 cells (with a single centrosome) to G2 cells (with 2 centrosomes) is increased by a factor of approximately 7 in Orc6³⁵ mutants.

Overall, the number of mitotic cells observed in Orc6³⁵ mutants decreased compared with wild-type or heterozygous tissues. No cells at anaphase or telophase stages are found in cells carrying the Orc6³⁵ deletion. Instead, a large number of metaphase-like figures are observed, in which highly condensed chromosomes are present with incomplete or disorganised alignments. In addition, individual chromatids appear aberrantly condensed and fragmented. Mitotic chromosomes in mutant neuroblasts appear shorter and thicker than in wild-type and sister chromatid cohesion is frequently lost. Extensive chromosome fragmentation is also observed.

Chromosomes from neuroblasts isolated from Orc6³⁵ mutants carrying Orc6^{ΔC.220.T:Avic\GFP} are able to incorporate BrdU, and therefore are able to replicate DNA. Brains observed in these larvae are significantly reduced in size compared with heterozygous or wild-type larval brains, suggesting severe defects in cell proliferation and larval brain development.

Orc6³⁵ mutant neuroblasts carrying Orc6^{ΔC.220.T:Avic\GFP} accumulate at mitosis. The number of mitotic neuroblasts derived from these transgenic flies increases approximately 4-fold compared with Orc6³⁵ mutant cells.

A large number of metaphase-like figures are observed in Orc6³⁵ mutant cells expressing Orc6^{ΔC.220.T:Avic\GFP}. Cells in other stages are also found (e.g. anaphase-like figures are found, containing broken chromosomes), some polyploid with multiple centrosomes, while others exhibit multiple centrosomes but don't have any DNA present.

Chromosomes from neuroblasts isolated from Orc6³⁵ mutants carrying Orc6^{W228A.K229A.T:Avic\GFP} are able to incorporate BrdU, and therefore are able to replicate DNA. Brains observed in these larvae are significantly reduced in size compared with heterozygous or wild-type larval brains, suggesting severe defects in cell proliferation and larval brain development.

Orc6³⁵ mutant neuroblasts carrying Orc6^{W228A.K229A.T:Avic\GFP} accumulate at mitosis. The number of mitotic neuroblasts derived from these transgenic flies increases approximately 8-fold compared with Orc6³⁵ mutant cells.

A large number of metaphase-like figures are observed in Orc6³⁵ mutant cells expressing Orc6^{W228A.K229A.T:Avic\GFP}. Cells in other stages are also found (e.g. anaphase-like figures are found, containing broken chromosomes), some polyploid with multiple centrosomes, while others exhibit multiple centrosomes but don't have any DNA present.

(Balasov et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Orc6³⁵ has decreased occurrence of cell division | larval stage phenotype, enhanceable by Hsap\ORC6^Orc6.UAS.GFP

(Balasov et al., 2009)

Suppressed by

Statement

Reference

Orc6³⁵ has increased mortality during development phenotype, suppressible | partially by Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL, Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL, Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL, Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has abnormal mitotic cell cycle | larval stage phenotype, suppressible by Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has abnormal mitotic cell cycle | larval stage phenotype, suppressible | partially by Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL, Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP

(Balasov et al., 2020)

Orc6³⁵ has abnormal mitotic cell cycle | larval stage phenotype, suppressible by Scer\GAL4^αTub84B.PL, Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP

(Balasov et al., 2020)

Orc6³⁵ has abnormal mitotic cell cycle phenotype, suppressible by Hsap\ORC6^Orc6.UAS.GFP

(Balasov et al., 2009)

NOT suppressed by

Statement

Reference

Orc6³⁵ has lethal phenotype, non-suppressible by Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP

(Balasov et al., 2020)

Orc6³⁵ has lethal phenotype, non-suppressible by Scer\GAL4^αTub84B.PL, Hsap\ORC6^{K23E.Orc6.UAS.GFP}/Hsap\ORC6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has abnormal mitotic cell cycle | larval stage phenotype, non-suppressible by Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP

(Balasov et al., 2020)

Orc6³⁵ has abnormal mitotic cell cycle | larval stage phenotype, non-suppressible by Scer\GAL4^αTub84B.PL, Hsap\ORC6^{K23E.Orc6.UAS.GFP}/Hsap\ORC6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has lethal phenotype, non-suppressible by Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has lethal phenotype, non-suppressible by Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has abnormal mitotic cell cycle | larval stage phenotype, non-suppressible by Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has lethal phenotype, non-suppressible by Hsap\ORC6^{K23E.Orc6.UAS.GFP}/Hsap\ORC6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has lethal | third instar larval stage phenotype, non-suppressible by Hsap\ORC6^Orc6.UAS.GFP

(Balasov et al., 2009)

Other

Statement

Reference

Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}, Orc6³⁵ has lethal phenotype

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}, Orc6³⁵ has abnormal neuroanatomy | larval stage phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has visible | adult stage phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has decreased size | larval stage phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵, Scer\GAL4^αTub84B.PL has partially lethal - majority die phenotype

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}, Orc6³⁵, Scer\GAL4^αTub84B.PL has partially lethal - majority die phenotype

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}, Orc6³⁵ has lethal phenotype

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}, Orc6³⁵ has abnormal neuroanatomy | larval stage phenotype

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}, Orc6³⁵ has decreased size | larval stage phenotype

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}, Orc6³⁵ has decreased size | larval stage phenotype

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}, Orc6³⁵ has partially lethal - majority live phenotype

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}, Orc6³⁵, Scer\GAL4^αTub84B.PL has partially lethal - majority die phenotype

(Balasov et al., 2020)

Hsap\ORC6^{K23E.Orc6.UAS.GFP}, Orc6³⁵ has lethal phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has lethal phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has abnormal neuroanatomy | larval stage phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has flightless phenotype

(Balasov et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

Orc6³⁵ has larval brain phenotype, enhanceable by Hsap\ORC6^Orc6.UAS.GFP

(Balasov et al., 2009)

Suppressed by

Statement

Reference

Orc6³⁵ has larval neuroblast phenotype, suppressible by Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has chromosome | larval stage phenotype, suppressible by Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has larval neuroblast phenotype, suppressible | partially by Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has chromosome | larval stage phenotype, suppressible | partially by Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has larval neuroblast phenotype, suppressible by Scer\GAL4^αTub84B.PL, Hsap\ORC6^{K23E.Orc6.UAS.GFP}/Hsap\ORC6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has chromosome | larval stage phenotype, suppressible by Scer\GAL4^αTub84B.PL, Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP

(Balasov et al., 2020)

NOT suppressed by

Statement

Reference

Orc6³⁵ has larval neuroblast phenotype, non-suppressible by Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has chromosome | larval stage phenotype, non-suppressible by Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has larval neuroblast phenotype, non-suppressible by Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP

(Balasov et al., 2020)

Orc6³⁵ has chromosome | larval stage phenotype, non-suppressible by Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP

(Balasov et al., 2020)

Orc6³⁵ has larval neuroblast phenotype, non-suppressible by Scer\GAL4^αTub84B.PL, Hsap\ORC6^{K23E.Orc6.UAS.GFP}/Hsap\ORC6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

Orc6³⁵ has chromosome | larval stage phenotype, non-suppressible by Scer\GAL4^αTub84B.PL, Hsap\ORC6^{K23E.Orc6.UAS.GFP}/Hsap\ORC6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

Other

Statement

Reference

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has larval brain | larval stage phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has wing phenotype

(Balasov et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

In the following genotypes, the larval brain is underdeveloped: Orc6³⁵/Orc6³⁵, Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}/ Orc6::Hsap\ORC6^{DH.Orc6.UAS.GFP}; Orc6³⁵/Orc6³⁵, Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP; Orc6³⁵/Orc6³⁵, Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}.

Orc6³⁵/Orc6³⁵, Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP surviving adults are flightless and exhibit upheld wings.

(Balasov et al., 2020)

Expression of Hsap\ORC6^T:Avic\GFP does not rescue viability in Orc6³⁵ mutants (that die at the third instar larval stage).

Expression of Hsap\ORC6^T:Avic\GFP results in the release of Orc6³⁵ mutant cells from G1 arrest, as shown by the ratio of cells containing either 1 or 2 centrosomes being indistinguishable from cells obtained from heterozygous siblings.

Chromosomes from neuroblasts isolated from Orc6³⁵ mutants carrying Hsap\ORC6^T:Avic\GFP are able to incorporate BrdU, and therefore are able to replicate DNA. Brains observed in these larvae are significantly reduced in size compared with heterozygous or wild-type larval brains, suggesting severe defects in cell proliferation and larval brain development.

Orc6³⁵ mutant neuroblasts carrying Hsap\ORC6^T:Avic\GFP accumulate at mitosis. The number of mitotic neuroblasts derived from these transgenic flies increases approximately 2-fold compared with Orc6³⁵ mutant cells.

(Balasov et al., 2009)

Complementation and Rescue Data

Rescued by

Orc6³⁵ is rescued by Orc6^+t3.3

(Balasov et al., 2009)

Orc6³⁵ is rescued by Orc6^Orc6.UAS.GFP

(Balasov et al., 2009)

Partially rescued by

FBal0365779:, Orc6³⁵ is partially rescued by Orc6^{K23E.Orc6.UAS.GFP}/Orc6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

FBal0365779:, Orc6³⁵ is partially rescued by Scer\GAL4^αTub84B.PL, Orc6^{K23E.Orc6.UAS.GFP}/Orc6^{K23E.Orc6.UAS.GFP}

(Balasov et al., 2020)

FBal0241168:, Orc6³⁵ is partially rescued by Scer\GAL4^αTub84B.PL, Orc6^Orc6.UAS.GFP/Orc6^Orc6.UAS.GFP

(Balasov et al., 2020)

FBal0241168:, Orc6³⁵ is partially rescued by Orc6^Orc6.UAS.GFP/Orc6^Orc6.UAS.GFP

(Balasov et al., 2020)

Orc6³⁵ is partially rescued by Orc6^{Y225S.Orc6.UAS.GFP}/Scer\GAL4^Tub.PU

(Balasov et al., 2015)

Orc6³⁵ is partially rescued by Orc6^Orc6.UAS.GFP/Scer\GAL4^Tub.PU

(Balasov et al., 2015)

Orc6³⁵ is partially rescued by Orc6^{Y225F.Orc6.UAS.GFP}

(Balasov et al., 2015)

Orc6³⁵ is partially rescued by Scer\GAL4^Tub.PU/Orc6^{Y225F.Orc6.UAS.GFP}

(Balasov et al., 2015)

Orc6³⁵ is partially rescued by Scer\GAL4^Tub.PU/Orc6^{D224A.Y225A.Orc6.UAS.GFP}

(Balasov et al., 2015)

Orc6³⁵ is partially rescued by Orc6^Orc6.UAS.GFP

(Balasov et al., 2015)

Not rescued by

Orc6³⁵ is not rescued by Orc6^{Y225S.Orc6.UAS.GFP}

(Balasov et al., 2015)

Orc6³⁵ is not rescued by Orc6^{D224A.Y225A.Orc6.UAS.GFP}

(Balasov et al., 2015)

Orc6³⁵ is not rescued by Scer\GAL4^Tub.PU/Orc6^{W228A.K229A.Orc6.UAS.GFP}

(Balasov et al., 2015)

Orc6³⁵ is not rescued by Scer\GAL4^Tub.PU/Orc6^{ΔC.200.Orc6.UAS.GFP}

(Balasov et al., 2015)

Orc6³⁵ is not rescued by Orc6^{ΔC.200.Orc6.UAS.GFP}

(Balasov et al., 2015)

Orc6³⁵ is not rescued by Orc6^{W228A.K229A.Orc6.UAS.GFP}

(Balasov et al., 2015)

Orc6³⁵ is not rescued by Orc6^{ΔC.220.Orc6.UAS.GFP}

(Balasov et al., 2009)

Orc6³⁵ is not rescued by Orc6^{ΔC.200.Orc6.UAS.GFP}

(Balasov et al., 2009)

Orc6³⁵ is not rescued by Orc6^{W228A.K229A.Orc6.UAS.GFP}

(Balasov et al., 2009)

Comments

Expression of Orc6^{Y225S.Orc6.Scer\UAS.T:Avic\GFP} under its native promoter fails to rescue the third instar lethality in Orc6³⁵ mutant larvae but expression under the control of Scer\GAL4^tub.PU can partially restore the viability of the Orc6³⁵ mutants. The rescued adults, however, display several phenotypic defects compared to controls including upheld wing posture, missing posterior scutellar bristles, rough spots in the eye and irregular hair pattern on the abdomen.

Larvae expressing Orc6^{Y225S.Orc6.Scer\UAS.T:Avic\GFP} under the control of its native promoter in Orc6³⁵ mutant background display decreased levels of DNA replication in both neural ganglia and testes compared to controls.

Expression of Orc6^{Y225S.Orc6.Scer\UAS.T:Avic\GFP} from its native promoter does not rescue the aberrant karyotype characteristic for Orc6³⁵ and the chromosomes in larval brain cells display a number of defects including loss of arms, fragmentation, polyploidy, aberrant condensation or misalignment during the cell cycle. This abnormal karyotype is however rescued when Orc6^{Y225S.Orc6.Scer\UAS.T:Avic\GFP} is expressed under the control of the Scer\GAL4^tub.PU driver.

Expression of Orc6^{Orc6.Scer\UAS.T:Avic\GFP} under the control of its native promoter or the Scer\GAL4^tub.PU driver almost completely restores the viability of Orc6³⁵ mutants. The abnormal brain cell karyotype as well as the DNA replication defects observed in brain and testes of Orc6³⁵ third instar larvae are also rescued by Orc6^{Orc6.Scer\UAS.T:Avic\GFP} expression from its native promoter.

Expression of Orc6^{Y225F.Orc6.Scer\UAS.T:Avic\GFP} under the control of its native promoter or Scer\GAL4^tub.PU in the Orc6³⁵ mutant background partially rescues the third instar stage lethality.

Expression of Orc6^{D224A.Y225A.Orc6.Scer\UAS.T:Avic\GFP} under its native promoter fails to rescue the third instar lethality in Orc6³⁵ mutant larvae but expression under the control of Scer\GAL4^tub.PU can partially restore the viability of the Orc6³⁵ mutants and rescues their aberrant karyotype.

Expression of Orc6^{W228A.K229A.Orc6.Scer\UAS.T:Avic\GFP} under the control of either its native promoter or the Scer\GAL4^tub.PU driver does not rescue either the third instar stage lethality or the aberrant karyotype of Orc6³⁵ mutant larvae.

Expression of Orc6^{ΔC.200.Orc6.Scer\UAS.T:Avic\GFP} under the control of either its native promoter or the Scer\GAL4^tub.PU driver does not rescue either the third instar stage lethality or the aberrant karyotype of Orc6³⁵ mutant larvae.

(Balasov et al., 2015)

Expression of Orc6^T:Avic\GFP results in the release of Orc6³⁵ mutant cells from G1 arrest, as shown by the ratio of cells containing either 1 or 2 centrosomes being indistinguishable from cells obtained from heterozygous siblings.

Expression of Orc6^+t3.3 results in the release of Orc6³⁵ mutant cells from G1 arrest, as shown by the ratio of cells containing either 1 or 2 centrosomes being indistinguishable from cells obtained from heterozygous siblings.

Expression of Orc6^{ΔC.220.T:Avic\GFP} results in the release of Orc6³⁵ mutant cells from G1 arrest, as shown by the ratio of cells containing either 1 or 2 centrosomes being indistinguishable from cells obtained from heterozygous siblings.

Expression of Orc6^{ΔC.200.T:Avic\GFP} fails to release of Orc6³⁵ mutant cells from G1 arrest.

Orc6³⁵ mutants carrying the Orc6^{ΔC.200.T:Avic\GFP} transgene do not differ from Orc6³⁵ mutant cells, in terms of having no BrdU incorporation in brain neuroblasts.

Expression of Orc6^{W228A.K229A.T:Avic\GFP} results in the release of Orc6³⁵ mutant cells from G1 arrest, as shown by the ratio of cells containing either 1 or 2 centrosomes being indistinguishable from cells obtained from heterozygous siblings.

(Balasov et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Orc6³⁵

(Goto et al., 2018)

orc6³⁵

(Balasov et al., 2025, Balasov et al., 2020, Bleichert et al., 2013, Balasov et al., 2009)

Δorc6

(Balasov et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (6)

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