FlyBase Allele Report: Hsap\ORC6[Orc6.UAS.GFP]

General Information

Symbol

Hsap\ORC6^Orc6.UAS.GFP

Species

H. sapiens

Name

FlyBase ID

FBal0241173

Feature type

allele

Associated gene

Hsap\ORC6

Associated Insertion(s)

Carried in Construct

P{Orc6-UAS-HsOrc6.N-GFP}

Key Links

Transgenic product class

wild_type

(Balasov et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Balasov et al., 2009)

Mutagen

in vitro construct

(Balasov et al., 2009)

Progenitor genotype

Carried in construct

P{Orc6-UAS-HsOrc6.N-GFP}

Cytology

Description

The native Orc6 promoter and UAS regulatory sequences are fused upstream of the full-length Hsap\ORC6 open reading frame, which is tagged at the N-terminal end with GFP.

(Balasov et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

Orc6

UAS

binary expression system - regulatory region

Encoded product / tool

Hsap\ORC6

Tagged with

GFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Meier-Gorlin syndrome 3

CEA

(Balasov et al., 2020)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Hsap\ORC6^Orc6.UAS.GFP is an enhancer of decreased occurrence of cell division | larval stage phenotype of Orc6³⁵

(Balasov et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^αTub84B.PL, Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP is a suppressor | partially of increased mortality during development phenotype of Orc6³⁵

(Balasov et al., 2020)

Scer\GAL4^αTub84B.PL, Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP is a suppressor of abnormal mitotic cell cycle | larval stage phenotype of Orc6³⁵

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP is a suppressor of abnormal mitotic cell cycle phenotype of Orc6³⁵

(Balasov et al., 2009)

NOT Suppressor of

Statement

Reference

Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP is a non-suppressor of lethal phenotype of Orc6³⁵

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP is a non-suppressor of abnormal mitotic cell cycle | larval stage phenotype of Orc6³⁵

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP is a non-suppressor of lethal | third instar larval stage phenotype of Orc6³⁵

(Balasov et al., 2009)

Other

Statement

Reference

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has lethal phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has abnormal neuroanatomy | larval stage phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has flightless phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has visible | adult stage phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has decreased size | larval stage phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵, Scer\GAL4^αTub84B.PL has partially lethal - majority die phenotype

(Balasov et al., 2020)

Phenotype Manifest In

Enhancer of

Statement

Reference

Hsap\ORC6^Orc6.UAS.GFP is an enhancer of larval brain phenotype of Orc6³⁵

(Balasov et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^αTub84B.PL, Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP is a suppressor of chromosome | larval stage phenotype of Orc6³⁵

(Balasov et al., 2020)

NOT Suppressor of

Statement

Reference

Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP is a non-suppressor of larval neuroblast phenotype of Orc6³⁵

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP is a non-suppressor of chromosome | larval stage phenotype of Orc6³⁵

(Balasov et al., 2020)

Other

Statement

Reference

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has wing phenotype

(Balasov et al., 2020)

Hsap\ORC6^Orc6.UAS.GFP, Orc6³⁵ has larval brain | larval stage phenotype

(Balasov et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The larval brain of Orc6³⁵/Orc6³⁵, Hsap\ORC6^Orc6.UAS.GFP/Hsap\ORC6^Orc6.UAS.GFP is underdeveloped. The surviving adults are flightless and exhibit upheld wings.

(Balasov et al., 2020)

Expression of Hsap\ORC6^T:Avic\GFP does not rescue viability in Orc6³⁵ mutants (that die at the third instar larval stage).

Expression of Hsap\ORC6^T:Avic\GFP results in the release of Orc6³⁵ mutant cells from G1 arrest, as shown by the ratio of cells containing either 1 or 2 centrosomes being indistinguishable from cells obtained from heterozygous siblings.

Chromosomes from neuroblasts isolated from Orc6³⁵ mutants carrying Hsap\ORC6^T:Avic\GFP are able to incorporate BrdU, and therefore are able to replicate DNA. b

Brains observed in these larvae are significantly reduced in size compared with heterozygous or wild-type larval brains, suggesting severe defects in cell proliferation and larval brain development.

Orc6³⁵ mutant neuroblasts carrying Hsap\ORC6^T:Avic\GFP accumulate at mitosis. The number of mitotic neuroblasts derived from these transgenic flies increases approximately 2-fold compared with Orc6³⁵ mutant cells.

(Balasov et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hsap\ORC6^{Orc6.Scer\UAS.T:Avic\GFP}

Hsap\ORC6^Orc6.UAS.GFP

Hsap\ORC6^T:Avic\GFP

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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