FB2026_02 , released June 18, 2026
Allele: Dmel\Orc6D224A.Y225A.Orc6.UAS.GFP
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General Information
Symbol
Dmel\Orc6D224A.Y225A.Orc6.UAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0319345
Feature type
allele
Associated gene
Dmel\Orc6
Associated Insertion(s)
Carried in Construct
P{Orc6-UAS-Orc6.N-GFP.D224A.Y225A}
Key Links
Transgenic product class
missense_variant
(Bleichert et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Bleichert et al., 2013)
Mutagen
in vitro construct
(Bleichert et al., 2013)
Progenitor genotype
Carried in construct
P{Orc6-UAS-Orc6.N-GFP.D224A.Y225A}
Cytology
Description

The native Orc6 promoter and UAS regulatory sequences are fused upstream of a mutated form of Orc6 (contains the amino acid replacements D224A, Y225A), which is tagged at the N-terminal end with GFP.

(Balasov et al., 2015)

Encodes a mutated form of Orc6 (contains the amino acid replacements D224A, Y225A), which is tagged at the N-terminal end with GFP.

(Bleichert et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
Orc6
UAS
UASt
Encoded product / tool
Orc6
Tagged with
GFP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4Tub.PU/Orc6D224A.Y225A.Orc6.UAS.GFP partially rescues Orc635

      (Balasov et al., 2015)
      Fails to rescue

      Orc6D224A.Y225A.Orc6.UAS.GFP fails to rescue Orc635

      (Balasov et al., 2015)
      Comments

      Expression of Orc6D224A.Y225A.Orc6.Scer\UAS.T:Avic\GFP under its native promoter fails to rescue the third instar lethality in Orc635 mutant larvae but expression under the control of Scer\GAL4tub.PU can partially restore the viability of the Orc635 mutants and rescues their aberrant karyotype.

      (Balasov et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Orc6D224A.Y225A.Orc6.Scer\UAS.T:Avic\GFP
      Orc6D224A.Y225A.Orc6.UAS.GFP
      Name Synonyms
      Secondary FlyBase IDs
        References (2)