FlyBase Allele Report: Dmel\Orc6::Hsap\ORC6[HDK23E.Orc6.UAS.GFP]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Allele: Dmel\Orc6::HsapORC6^{HDK23E.Orc6.UAS.GFP}

General Information

Symbol

Dmel\Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0365783

Feature type

allele

Associated gene

Orc6::Hsap\ORC6

Associated Insertion(s)

Carried in Construct

P{Orc6-UAS-HDK23E.N-GFP}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Balasov et al., 2020)

Progenitor genotype

Carried in construct

P{Orc6-UAS-HDK23E.N-GFP}

Cytology

Description

The native Orc6 promoter and UASt regulatory sequences are fused upstream of a hybrid protein composed of the N-terminal 206 amino acids of Hsap\ORC6 fused to the C-terminal 52 amino acids of Orc6. The protein contains a K23E amino acid substitution, a variant identified in a patient with Meier-Gorlin syndrome. The coding sequence is tagged at the N-terminal end with GFP.

(Balasov et al., 2020)

Allele components

Component

Use(s)

Regulatory region(s)

binary expression system - regulatory region

Encoded product / tool

Orc6::Hsap\ORC6

Tagged with

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Meier-Gorlin syndrome 3

CEA

(Balasov et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of Meier-Gorlin syndrome 3

is ameliorated by Orc6^Orc6.UAS.GFP

(Balasov et al., 2020)

is ameliorated by Orc6::Hsap\ORC6^{HD.Orc6.UAS.GFP}

(Balasov et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Balasov et al., 2020)

ORC6:p.Lys23Glu

(FlyBase curators, 2019-)

Variants Synonym(s)

Associated human disease model(s)

Meier-Gorlin syndrome 3

External database links

Comments concerning this variant

Phenotypic Data

Phenotypic Class

partially lethal - majority live, with Scer\GAL4^αTub84B.PL

(Balasov et al., 2020)

Phenotype Manifest In

larval brain | larval stage

(Balasov et al., 2020)

Detailed Description

Statement

Reference

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^αTub84B.PL, Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP} is a suppressor | partially of increased mortality during development phenotype of Orc6³⁵

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP} is a suppressor | partially of abnormal mitotic cell cycle | larval stage phenotype of Orc6³⁵

(Balasov et al., 2020)

NOT Suppressor of

Statement

Reference

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP} is a non-suppressor of lethal phenotype of Orc6³⁵

(Balasov et al., 2020)

Other

Statement

Reference

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}, Orc6³⁵, Scer\GAL4^αTub84B.PL has partially lethal - majority die phenotype

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}, Orc6³⁵ has lethal phenotype

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}, Orc6³⁵ has abnormal neuroanatomy | larval stage phenotype

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}, Orc6³⁵ has decreased size | larval stage phenotype

(Balasov et al., 2020)

Phenotype Manifest In

Suppressor of

Statement

Reference

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP} is a suppressor | partially of larval neuroblast phenotype of Orc6³⁵

(Balasov et al., 2020)

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP} is a suppressor | partially of chromosome | larval stage phenotype of Orc6³⁵

(Balasov et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The larval brain of Orc6³⁵/Orc6³⁵, Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}/Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP} is underdeveloped.

(Balasov et al., 2020)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Orc6::Hsap\ORC6^{HDK23E.Orc6.UAS.GFP}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections