FlyBase Allele Report: Dmel\larp[S022529]

General Information

Symbol

Dmel\larp^S022529

Species

D. melanogaster

Name

FlyBase ID

FBal0241444

Feature type

allele

Associated gene

Dmel\larp

Associated Insertion(s)

P{lacW}larp^S022529

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}larp^S022529

Cytology

Description

P{lacW} insertion in the first intron.

(Ichihara et al., 2007)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle (with larp^mtr-null)

(Blagden et al., 2009)

female sterile

(Ichihara et al., 2007)

female sterile (with larp^mtr-null)

(Blagden et al., 2009)

male sterile

(Ichihara et al., 2007)

male sterile (with larp^mtr-null)

(Blagden et al., 2009)

partially lethal - majority die | recessive

(Deak et al., 1997)

Phenotype Manifest In

centrosome (with larp^mtr-null)

(Blagden et al., 2009)

embryo | embryonic stage 4 (with larp^mtr-null)

(Blagden et al., 2009)

Nebenkern (with larp⁰⁶⁴⁸⁷)

(Ichihara et al., 2007)

onion stage spermatid (with larp⁰⁶⁴⁸⁷)

(Ichihara et al., 2007)

spindle (with larp^mtr-null)

(Blagden et al., 2009)

Detailed Description

Statement

Reference

45% of larp^S022529/larp^mtr-null females are sterile.

Eggs laid by larp^S022529/larp^mtr-null females show a number of defects during syncytial divisions, including chains of linked spindles and free centrosomes. Time-lapse imaging shows that centrosomes can be seen to successfully duplicate following the previous division cycle, however the movement of the centrosomes around the nuclear membrane is hindered. Nonetheless, a spindle forms between the two groups of centrosomes. Nuclear spacing is also disrupted.

(Blagden et al., 2009)

31.4% of onion stage spermatids show abnormalities in larp^mtr1/larp⁰⁶⁴⁸⁷ males. Of these abnormal cells, 52.0% show abnormal cell numbers or size, 35.0% have abnormal Nebenkern numbers or size, 13.0% show a failure of cytokinesis and 3.4% have multiple abnormalities.

(Ichihara et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Allelic series (severity of mutant alleles): larp^mtr-null > larp^S003108 > larp^S022529 > larp⁰⁶⁴⁸⁷.

(Blagden et al., 2009)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

0225/29

(Deak et al., 1997)

l(3)S022529^S022529

larp^S022529

larp<up>mtr 1</up>

(Blagden et al., 2009)

larp^mtr1

(Ichihara et al., 2007)

mtr¹

(Ichihara et al., 2007)

Name Synonyms

Secondary FlyBase IDs

FBal0220946
FBal0130029

References (3)

Report Sections

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