FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Hsap\APPAPP.LV.UAS.Tag:MYC,Tag:NLS(SV40-largeT)
Open Close
General Information
Symbol
Hsap\APPAPP.LV.UAS.Tag:MYC,Tag:NLS(SV40-largeT)
Species
H. sapiens
Name
FlyBase ID
FBal0241700
Feature type
allele
Associated gene
Hsap\APP
Associated Insertion(s)
Carried in Construct
P{UAS-mycAPP.LV}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Loewer et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-mycAPP.LV}
Cytology
Description

UAS sequences drive expression of a fusion protein composed of the Hsap\APP coding sequence, nuclear localization sequence (Tag:NLS(SV40-largeT)) and a lexA::VP16 driver sequence. The protein is also tagged at the end with a Tag:MYC epitope. This protein acts as a reporter of processing of Hsap\APP in vivo. In the absence of processing the lexA::VP16 driver is tethered to the membrane by Hsap\APP. Upon processing of Hsap\APP by α-secretase and Presenilin, a protein fragment containing the nuclear localization sequence and lexA::VP16 driver is released, which is then able to translocate to the nucleus and drive expression of a lexAop-driven reporter transgene.

(Loewer et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Hsap\APP
Tagged with
Tag:MYC
Tag:NLS(SV40-largeT)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Hsap\APPAPP.LV.UAS.Tag:MYC,Tag:NLS(SV40-largeT)
      Hsap\APPScer\UAS.T:Hsap\MYC,T:SV40\nls-largeT,T:Ecol\lexA.T:Hsim\VP16
      Hsap\APPScer\UAS.T:Hsap\MYC,T:SV40\nls2,T:Ecol\lexA.T:Hsim\VP16
      Hsap\APPScer\UAS.T:Hsap\MYC.T:SV40\nls2.T:Ecol\lexA.T:Hsim\VP16
      Name Synonyms
      Secondary FlyBase IDs
        References (4)