FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Syt4wt.UAS
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General Information
Symbol
Dmel\Syt4wt.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0241913
Feature type
allele
Associated gene
Dmel\Syt4
Associated Insertion(s)
Carried in Construct
P{UAS-Syt4.wt}
Key Links
Transgenic product class
wild_type
(Barber et al., 2009)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Barber et al., 2009)
Mutagen
in vitro construct
(Barber et al., 2009)
Progenitor genotype
Carried in construct
P{UAS-Syt4.wt}
Cytology
Description

UASt regulatory sequences drive expression of wild-type full-length Syt4 coding sequences.

(Barber et al., 2009)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Syt4
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Syt4wt.Scer\UAS post-synaptically under the control of Scer\GAL4Mhc.PW results in an approximate 60% increase in bouton number.

      Expression of Syt4wt.Scer\UAS post-synaptically under the control of Scer\GAL4Mhc.PW results in enhanced spontaneous neurotransmitter release in the absence of nerve stimulation and enhanced evoked neurotransmission (at 0.5mM Ca[2+] concentration). These changes in evoked responses are not observed in 2.0mM extracellular Ca[2+] concentration, suggesting that alterations in release probability or vesicle pool size may compensate for the altered number of release sites. The fast component of synaptic depression release during initial stimulation is unchanged. However, overexpression larvae show more depression and a smaller steady-state response during the train.

      Overexpression of Syt4wt.Scer\UAS post-synaptically under the control of Scer\GAL4Mhc.PW triggers an elevation in the frequency of spontaneous fusion after high frequency stimulation compared with wild-type synapses, with spontaneous release rates often >15Hz.

      (Barber et al., 2009)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4Mhc.PW/Syt4wt.UAS rescues Df(3R)rn16/Syt4BA1

      (Barber et al., 2009)
      Comments

      Expression of Syt4wt.Scer\UAS post-synaptically under the control of Scer\GAL4Mhc.PW rescues the Syt4BA1/Df(3R)rn16 synaptic growth defect.

      (Barber et al., 2009)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Syt4wt.Scer\UAS
      Syt4wt.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)