FlyBase Allele Report: Dmel\kay[NIG.15507R]

General Information

Symbol

Dmel\kay^NIG.15507R

Species

D. melanogaster

Name

FlyBase ID

FBal0243041

Feature type

allele

Associated gene

Dmel\kay

Associated Insertion(s)

Carried in Construct

P{NIG.15507R}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Rallis et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Rallis et al., 2010)

Mutagen

in vitro construct

(Rallis et al., 2010)

Progenitor genotype

Carried in construct

P{NIG.15507R}

Cytology

Description

UASt sequences drive expression of a kay RNAi construct.

(Rallis et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-15507R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm, with Dcr-2^UAS.cDa, Scer\GAL4^P2.4.Pdf

(Ling et al., 2012)

abnormal locomotor rhythm, with Scer\GAL4^P2.4.Pdf

(Ling et al., 2012)

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

(Rallis et al., 2010)

Phenotype Manifest In

axon, with Scer\GAL4^ey-OK107

(Rallis et al., 2010)

Kenyon cell, with Scer\GAL4^ey-OK107

(Rallis et al., 2010)

Detailed Description

Statement

Reference

Flies expressing kay^NIG.15507R under the control of Scer\GAL4^P2.4.Pdf under constant darkness conditions show a lengthening of the locomotor activity period by approximately 1 hour compared to controls. The period lengthening is increased to approximately 1.5 hours if the flies also carry Dcr-2^Scer\UAS.cDa (to increase RNAi efficiency), and the proportion of arrhythmic flies is also increased.

s-LNv neurons develop normally in flies expressing kay^NIG.15507R under the control of Scer\GAL4^P2.4.Pdf in the presence of Dcr-2^Scer\UAS.cDa.

(Ling et al., 2012)

Expression of kay^NIG.15507R using Scer\GAL4^ey-OK107 results in axon overextension.

(Rallis et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Dcr-2^UAS.cDa, Scer\GAL4^P2.4.Pdf, kay^NIG.15507R has abnormal locomotor rhythm phenotype, non-suppressible by Pdf^UAS.cRa, Scer\GAL4^P2.4.Pdf

(Ling et al., 2012)

Other

Statement

Reference

Dcr-2^UAS.cDa, Scer\GAL4^ey-OK107, kay^NIG.15507R has abnormal neuroanatomy phenotype

(Rallis et al., 2010)

Phenotype Manifest In

Other

Statement

Reference

Dcr-2^UAS.cDa, Scer\GAL4^ey-OK107, kay^NIG.15507R has axon phenotype

(Rallis et al., 2010)

Dcr-2^UAS.cDa, Scer\GAL4^ey-OK107, kay^NIG.15507R has Kenyon cell phenotype

(Rallis et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The long period phenotype seen in flies expressing kay^NIG.15507R under the control of Scer\GAL4^P2.4.Pdf in the presence of Dcr-2^Scer\UAS.cDa (which increases RNAi efficiency) is not rescued by co-expression of Pdf^Scer\UAS.cRa.

(Ling et al., 2012)

Expression of kay^NIG.15507R and Dcr-2^Scer\UAS.cDa using Scer\GAL4^ey-OK107 results in axon degeneration.

(Rallis et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

kay^NIG.15507R is rescued by Dcr-2^UAS.cDa/kay::Dpse\kay^UAS.α/Scer\GAL4^P2.4.Pdf

(Ling et al., 2012)

Not rescued by

kay^NIG.15507R is not rescued by Dcr-2^UAS.cDa/Dpse\kay^UAS.trunc/Scer\GAL4^P2.4.Pdf

(Ling et al., 2012)

Comments

Co-expression of kay::Dpse\kay^Scer\UAS.α rescues the long period phenotype seen in flies expressing kay^NIG.15507R under the control of Scer\GAL4^P2.4.Pdf in the presence of Dcr-2^Scer\UAS.cDa (which increases RNAi efficiency).

Co-expression of Dpse\kay^{Scer\UAS.trunc} fails to rescue the long period phenotype seen in flies expressing kay^NIG.15507R under the control of Scer\GAL4^P2.4.Pdf in the presence of Dcr-2^Scer\UAS.cDa (which increases RNAi efficiency).

(Ling et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

15507R-2

P{NIG.15507R}2

15507R-4

P{NIG.15507R}4

Notes on Origin

Discoverer