FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\LamD395
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General Information
Symbol
Dmel\LamD395
Species
D. melanogaster
Name
FlyBase ID
FBal0243196
Feature type
allele
Associated gene
Dmel\Lam
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Muñoz-Alarcón et al., 2007)
Progenitor genotype
LamP
(Muñoz-Alarcón et al., 2007)
Cytology
Description

Imprecise excision of P{lacW}LamP generates LamD395, where exon 2, containing the translation start codon, is removed.

(Muñoz-Alarcón et al., 2007)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
2L:5,542,983..5,544,129 [+]
Comment:

Deletion resulting from the imprecise excision of P{lacW}LamP. The downstream deletion boundary within exon 3 of Lam was estimated from the map in Fig. 2.

(Muñoz-Alarcón et al., 2007)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Five day-old LamD395/LamK2 mutant flies exhibit severe midgut hyperplasia, equivalent to that seen in 50 day-old wild type flies.

      The eclosion rate of LamD395 mutant flies is increased when the flies are raised in axenic conditions.

      Suppressing secretion in fat body cells expressing Scer\GAL4Cg.PA results in a reduction in gut hyperplasia in LamD395/LamK2 mutant flies.

      (Chen et al., 2014)

      Most gonads of LamD395/Df(2L)cl-h1 male larvae are small, being filled with early germ cells and almost completely lacking differentiated spermatocytes and spermatids. The mutant early germ cells contain spectrosome-like structures and poorly branched fusome-like structures. Only 2.4% of the mutant His3-positive cysts contain 4 or 8 germ cells (in contrast to 52.2% of His3-positive cysts in wild type) and the cysts are distributed throughout the gonad. The nuclei of many cyst stem cells in the mutant gonad are positioned adjacent to hub cells (in wild type the nuclei are dispersed away from the hub), while the number of germline stem cells associated with the hub is decreased compared to wild type. Cyst cells are displaced away from germ cells in the mutant gonad, with many being mislocalised to the surface.

      (Chen et al., 2013)

      LamD395/Df(2L)cl-h1 trans-heterozygotes exhibit less and later lethality than LamD395 homozygotes, suggesting the presence of other genetic aberrations.

      LamD395/Df(2L)cl-h1 trans-heterozygous larvae move significantly less than controls or LamD395/+ heterozygotes.

      LamD395 homozygous and heterozygous mutants exhibit lower pupariation height, indicative of reduced larval locomotion and negative gravitaxis.

      (Muñoz-Alarcón et al., 2007)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of keyGD1249 under the control of Scer\GAL4Cg.PA suppresses the increased intestinal hyperplasia seen in LamD395/LamK2 mutant flies.

      Co-expression of PGRP-LBScer\UAS.T:Avic\GFP-YFP and PGRP-SC2Scer\UAS.cCa under the control of Scer\GAL4Cg.PA suppresses the increased intestinal hyperplasia seen in LamD395/LamK2 mutant flies.

      (Chen et al., 2014)

      Expression of rlScer\UAS.T:Uuuu\nls6 under the control of Scer\GAL4tj-NP1624 rescues the defects in germline stem cell differentiation seen in LamD395/Df(2L)cl-h1 males. In 40.6% of testes spermatocytes are formed and the gonads do not accumulate many early germline cells. The mislocalisation of the cyst stem cell nuclei adjacent to the hub and the reduction in number of germline stem cells associated with the hub are also partially rescued.

      (Chen et al., 2013)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescued by

      LamK2/LamD395 is partially rescued by LamUAS.cMAa

      (Chen et al., 2014)
      Comments

      Expression of LamScer\UAS.cMAa in the fat body under the control of Scer\GAL4Cg.PA significantly rescues the intestinal hyperplasia seen in LamD395/LamK2 mutant flies. The eclosion rate is increased from ~8% to ~40%.

      (Chen et al., 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Based on lethality, the following alleles can form an allelic class, from the most severe to the weakest: LamP; LamD395; Lam04643; LamG262.

      (Muñoz-Alarcón et al., 2007)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      LamD395
      (Chen et al., 2013)
      Name Synonyms
      Secondary FlyBase IDs
        References (3)