FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\EndoAA66W.UAS
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General Information
Symbol
Dmel\EndoAA66W.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0243622
Feature type
allele
Associated gene
Dmel\EndoA
Associated Insertion(s)
Carried in Construct
P{UAS-EndoA.A66W}
Key Links
Transgenic product class
missense_variant
(Jung et al., 2010)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Jung et al., 2010)
Mutagen
in vitro construct
(Jung et al., 2010)
Progenitor genotype
Carried in construct
P{UAS-EndoA.A66W}
Cytology
Description

UAS drives expression of the GH12907 EndoA cDNA (in which lysine at position 129 has been changed to arginine in order to comply with the EndoA 'consensus sequence' in FlyBase), containing an A66W amino acid change.

(Jung et al., 2010)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
EndoA
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Depending on the insertion, Scer\GAL4elav-C155-driven expression of endoAA66W.Scer\UAS causes embryonic or pupal lethality, or flies die a few days after eclosion exhibiting uninflated wings and a small-eye phenotype. Additional eye phenotypes in flies surviving to adulthood include: unusually narrow and pointed aspect of the lower eye; roughening of the eye caused by ommatidia that are uneven in size and missing/supernumerary bristles; abnormal pitting in the ommatidia.

      (Jung et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Partially rescues

      Scer\GAL4elav-C155/EndoAA66W.UAS partially rescues EndoAΔ4

      (Jung et al., 2010)
      Comments

      Expression of endoAA66W.Scer\UAS using Scer\GAL4elav-C155 doesn't completely rescue endoAΔ4 larval lethality, though animals can survive to pupal or pharate stages, depending on the insertion. This does rescue the severe tetanus-induced depression in excitatory junctional potentials (EJPs), the slow post-tetanic recovery of EJP amplitude, and the low frequency of spontaneous miniature EJPs seen in endoAΔ4 mutants.

      (Jung et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      EndoAA66W.Scer\UAS
      EndoAA66W.UAS
      endoAA66W.Scer\UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (1)