FlyBase Allele Report: Dmel\shi[ts1.QUAS]

General Information

Symbol

Dmel\shi^ts1.QUAS

Species

D. melanogaster

Name

FlyBase ID

FBal0243708

Feature type

allele

Associated gene

Dmel\shi

Associated Insertion(s)

Carried in Construct

P{QUAS-shi.ts1}

Also Known As

QUAS-shi^ts, QUAS-shi^ts1

Key Links

Transgenic product class

missense_variant

(Potter et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Potter et al., 2010)

Mutagen

in vitro construct

(Potter et al., 2010)

Progenitor genotype

Carried in construct

P{QUAS-shi.ts1}

Cytology

Description

QUAS regulatory sequences (followed by a minimal Hsp70 promoter) drive expression of a temperature sensitive form of shi (derived from the shi¹ mutant allele).

(Potter et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

QUAS

binary expression system - regulatory region

Encoded product / tool

shi

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies carrying shi^{ts1.Ncra\QUAS} under the control of Ncra\QF^ET49 become paralysed within 20 seconds of being placed at 37[o]C. They show full recovery within 5 minutes after being returned to the permissive temperature.

(Potter et al., 2010)

Flies expressing shi^{ts1.Ncra\QUAS} in all GH146-positive olfactory projection neurons under the control of Ncra\QF^GH146.PP show a significant reduction in olfactory attraction to ethyl acetate compared to wild-type controls.

Flies expressing shi^{ts1.Ncra\QUAS} in only lateral/ventral GH146-positive olfactory projection neurons under the control of Ncra\QF^GH146.PP (the flies contain Ncra\QS^Scer\UAS.cPa expressed under the control of Scer\GAL4^acj6-PG63, which prevents expression of shi^{ts1.Ncra\QUAS} via Ncra\QF^GH146.PP in the Scer\GAL4^acj6-PG63 expression domain, limiting expression to the lateral/ventral GH146-positive olfactory projection neurons) show a significant reduction in olfactory attraction to ethyl acetate compared to wild-type controls.