FlyBase Allele Report: Dmel\robo1[KO.robo2]

General Information

Symbol

Dmel\robo1^KO.robo2

Species

D. melanogaster

Name

FlyBase ID

FBal0243803

Feature type

allele

Associated gene

Dmel\robo1

Associated Insertion(s)

TI{TI}robo1^KO.robo2

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Spitzweck et al., 2010)

I-SceI

(Spitzweck et al., 2010)

FLPase

(Spitzweck et al., 2010)

I-CreI

(Spitzweck et al., 2010)

ends-in gene targeting

(Spitzweck et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{TI}robo1^KO.robo2

Cytology

Description

The exons (and intervening small introns) that encode the mature robo1 protein have been replaced with a single exon encoding full length robo2 tagged with Tag:HA. The robo1^KO.robo2 allele represents the loss of the robo1 open reading frame at the endogenous robo1 locus, while the robo2^{robo1.HR.Tag:HA} allele represents the mature robo2 protein tagged with Tag:HA that is expressed under the control of the robo1 regulatory sequences in place of robo1.

(Spitzweck et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

robo1^KO.robo2, robo2^{robo1.HR.Tag:HA} has viable phenotype

(Spitzweck et al., 2010)

robo1^KO.robo2, robo2^{robo1.HR.Tag:HA} has fertile phenotype

(Spitzweck et al., 2010)

robo1^KO.robo2, robo2^{robo1.HR.Tag:HA} has abnormal neuroanatomy | embryonic stage phenotype

(Spitzweck et al., 2010)

Phenotype Manifest In

Other

Statement

Reference

robo1^KO.robo2, robo2^{robo1.HR.Tag:HA} has larval longitudinal connective phenotype

(Spitzweck et al., 2010)

robo1^KO.robo2, robo2^{robo1.HR.Tag:HA} has pCC neuron phenotype

(Spitzweck et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Fas2-positive longitudinal fascicles of stage 16 robo^KO.lea lea^{robo.HR.T:Ivir\HA1} double homozygous embryos show midline crossing defects that are qualitatively and quantitatively similar to those seen in robo mutant embryos (Fas2-positive longitudinal fascicles extend across or along the midline in 97.6% of segments). In stage 13 robo^KO.lea lea^{robo.HR.T:Ivir\HA1} double homozygous embryos, the pCC axons project aberrantly across the midline, as occurs in robo mutant embryos.

(Spitzweck et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

97228

y¹ w^*; TI{TI}robo1^KO.robo2/CyO, P{en1}wg^en11

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

robo1^KO.lea

robo1^KO.robo2

robo1^robo2

(Spitzweck et al., 2010)

robo^KO.lea

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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