Contains 2386bp of the robo1 5' flanking region (coordinates 2R:18589619..18587234 , release 5 genome), which includes the region encoding the robo1 signal peptide up to residue Q52, 4 tandem Tag:HA tags, intronless sequence encoding the open reading frame of robo1 (starting at residue Y53 and ending at the stop codon) and 2177bp of the robo1 3' flanking region (nucleotides 2R:18582049..18579873 , release 5 genome).
roborobo.T:Ivir\HA1 almost fully rescues the midline crossing errors seen in Fas2-expressing longitudinal axons of robo1 homozygous embryos.