UAS regulatory sequences drive expression of a truncated form of Rca1 (lacking the amino-terminal region, including the F-Box), tagged at the C-terminus with Tag:HA and Tag:FLAG.
Scer\GAL4prd.RG1/Rca1Δ133.UAS.Tag:HA.Tag:FLAG rescues Rca12
Expression of Rca1Δ133.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG under the control of Scer\GAL4prd.RG1 in a Rca12 mutant background restores embryonic cell numbers to wild-type levels in Scer\GAL4prd.RG1-expressing segments.