FlyBase Allele Report: Dmel\tefu[RNAi.UAS]

General Information

Symbol

Dmel\tefu^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0244112

Feature type

allele

Associated gene

Dmel\tefu

Associated Insertion(s)

Carried in Construct

P{UAS-tefu.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Rimkus et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Rimkus et al., 2008)

Mutagen

in vitro construct

(Rimkus et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-tefu.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a 642 bp fragment corresponding to exon 6 (nucleotides 1185-1827) of tefu.

(Rimkus et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

tefu

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of ataxia telangiectasia

CEA

(Petersen et al., 2012, Rimkus et al., 2008)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of ataxia telangiectasia

is exacerbated by Delta⁰⁵¹⁵¹

(Rimkus et al., 2008)

is ameliorated by HDAC1⁰⁴⁵⁵⁶

(Rimkus et al., 2008)

is exacerbated by Mekk1^EY02276

(Rimkus et al., 2008)

is ameliorated by rad50^EP1

(Rimkus et al., 2008)

is ameliorated by stg^EY12388

(Rimkus et al., 2008)

is exacerbated by wrd^A131

(Rimkus et al., 2008)

is ameliorated by BacA\p35^GMR.PH

(Rimkus et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(Petersen et al., 2012)

abnormal locomotor behavior | adult stage, with Scer\GAL4^repo

(Petersen et al., 2013, Petersen et al., 2012)

abnormal neuroanatomy, with Scer\GAL4^elav.PU

(Rimkus et al., 2008)

abnormal neuroanatomy, with Scer\GAL4^GMR.PU

(Rimkus et al., 2008)

abnormal neuroanatomy | adult stage, with Scer\GAL4^elav-C155

(Petersen et al., 2012)

abnormal neuroanatomy | adult stage, with Scer\GAL4^repo

(Petersen et al., 2012)

abnormal neuroanatomy | temperature conditional, with Scer\GAL4^hs.PB

(Rimkus et al., 2008)

decreased cell death | third instar larval stage, with Scer\GAL4^elav.PU

(Rimkus et al., 2008)

increased cell death | adult stage, with Scer\GAL4^repo

(Petersen et al., 2012)

increased cell death | third instar larval stage, with Scer\GAL4^elav.PU

(Rimkus et al., 2008)

increased occurrence of cell division | larval stage, with Scer\GAL4^GMR.PU

(Rimkus et al., 2008)

long lived, with Scer\GAL4^elav.PLu

(Petersen et al., 2012)

short lived, with Scer\GAL4^repo

(Petersen et al., 2012)

Phenotype Manifest In

adult optic lobe, with Scer\GAL4^elav-C155

(Petersen et al., 2012)

eye, with Scer\GAL4^GMR.PU

(Rimkus et al., 2008)

eye disc posterior to the morphogenetic furrow | third instar larval stage, with Scer\GAL4^elav.PU

(Rimkus et al., 2008)

eye disc posterior to the morphogenetic furrow | third instar larval stage, with Scer\GAL4^GMR.PU

(Rimkus et al., 2008)

eye photoreceptor cell | temperature conditional, with Scer\GAL4^hs.PB

(Rimkus et al., 2008)

eye photoreceptor cell | third instar larval stage, with Scer\GAL4^elav.PU

(Rimkus et al., 2008)

eye photoreceptor cell | third instar larval stage, with Scer\GAL4^GMR.PU

(Rimkus et al., 2008)

glial cell, with Scer\GAL4^repo

(Petersen et al., 2012)

lamina, with Scer\GAL4^repo

(Petersen et al., 2012)

neuron, with Scer\GAL4^repo

(Petersen et al., 2012)

neuropil, with Scer\GAL4^repo

(Petersen et al., 2012)

rhabdomere of eye photoreceptor cell, with Scer\GAL4^elav.PU

(Rimkus et al., 2008)

rhabdomere of eye photoreceptor cell, with Scer\GAL4^GMR.PU

(Rimkus et al., 2008)

Detailed Description

Statement

Reference

Flies show expressing tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^repo reduced climbing ability compared to wild type. In a countercurrent assay, 70% of the mutant flies are poor climbers, 16% moderate climbers and only 14% are good climbers (compared to wild-type values of 13% poor/moderate climbers and 87% good climbers). Flies which are poor climbers have significantly shorter longevity than good climbers, with the moderate climbers having intermediate longevity, suggesting that the difference in climbing ability between individual flies is due to differences in the severity of neurodegeneration.

(Petersen et al., 2013)

Flies expressing tefu^{dsRNA.Scer\UAS} in glial cells under the control of Scer\GAL4^repo exhibit climbing defects. They also exhibit significantly reduced longevity compared to controls, and unlike in wild type, holes are seen in the adult lamina and neuropil. Significantly more apoptotic (Caspase 3 positive) cells are seen than in wild type. At 7 days these are limited to neurons, but also include glial cells at 17 days. Mobility, longevity, brain morphology of apoptosis levels are not adversely affected when tefu^{dsRNA.Scer\UAS} is expressed in neurons under the control of Scer\GAL4^elav.PLu, and flies have increased lifespan compared to controls. Lifespan and apoptosis levels are not affected when tefu^{dsRNA.Scer\UAS} is expressed using Scer\GAL4^elav-C155, but a slight reduction in climbing ability is observed in young flies. No holes are seen in the brain, but several round structures with a darkly stained interior core are observed in the optic lobe.

(Petersen et al., 2012)

Expression of tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav.PU results in neurodegeneration phenotypes. Swelling, fragmentation and loss of rhabdomeres in 3-day old adult eyes is observed.

Expression of tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU results in neurodegeneration phenotypes. Swelling, fragmentation and loss of rhabdomeres in 3-day old adult eyes is observed, and the extent of neurodegeneration becomes more severe with age. Eyes from these animals show a rough-eye phenotype.

Expression of tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^hs.PB during development (animals heat-shocked twice daily from egg-laying until day 3), or during adulthood (adults heat-shocked twice daily from day 3 to day 15), both result in degeneration of adult photoreceptor neurons.

Dividing photoreceptor neurons are observed posterior to the morphogenetic furrow in third instar larval eye discs expressing tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU or Scer\GAL4^elav.PU.

Reduced cell death directly posterior to the morphogenetic furrow, and increased cell death more posterior to the morphogenetic furrow is observed in third instar larval eye discs expressing tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU. A small number of these dying cells are neurons.

(Rimkus et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has abnormal neuroanatomy phenotype, suppressible by BacA\p35^GMR.PH

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has abnormal neuroanatomy phenotype, suppressible by stg[+]/stg^EY12388

(Rimkus et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye phenotype, enhanceable by Mekk1[+]/Mekk1^EY02276

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye photoreceptor cell phenotype, enhanceable by Mekk1[+]/Mekk1^EY02276

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has ommatidium phenotype, enhanceable by Mekk1[+]/Mekk1^EY02276

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye phenotype, enhanceable by Delta⁰⁵¹⁵¹/Dl[+]

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye photoreceptor cell phenotype, enhanceable by Delta⁰⁵¹⁵¹/Dl[+]

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has ommatidium phenotype, enhanceable by Delta⁰⁵¹⁵¹/Dl[+]

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye phenotype, enhanceable by PP2A-B'[+]/wrd^A131

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye photoreceptor cell phenotype, enhanceable by PP2A-B'[+]/wrd^A131

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has ommatidium phenotype, enhanceable by PP2A-B'[+]/wrd^A131

(Rimkus et al., 2008)

Suppressed by

Statement

Reference

Scer\GAL4^elav.PU, tefu^RNAi.UAS has eye photoreceptor cell | third instar larval stage phenotype, suppressible by stg[+]/stg^EY12388

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye phenotype, suppressible by stg[+]/stg^EY12388

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye photoreceptor cell phenotype, suppressible by stg[+]/stg^EY12388

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye phenotype, suppressible by HDAC1⁰⁴⁵⁵⁶/Rpd3[+]

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye photoreceptor cell phenotype, suppressible by HDAC1⁰⁴⁵⁵⁶/Rpd3[+]

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye phenotype, suppressible by rad50[+]/rad50^EP1

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye photoreceptor cell phenotype, suppressible by rad50[+]/rad50^EP1

(Rimkus et al., 2008)

Scer\GAL4^GMR.PU, tefu^RNAi.UAS has eye phenotype, suppressible by BacA\p35^GMR.PH

(Rimkus et al., 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^elav.PU, tefu^RNAi.UAS has eye disc | third instar larval stage phenotype, non-suppressible by BacA\p35^GMR.PH

(Rimkus et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

stg^EY12388/+ suppresses the rough-eye phenotype and increases the number of normal photoreceptors per ommatidium in flies expressing tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU. Significantly reduced severity of progressive neurodegeneration is also observed in these animals.

Rpd3⁰⁴⁵⁵⁶/+ suppresses the rough-eye phenotype and increases the number of normal photoreceptors per ommatidium in flies expressing tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU.

rad50^EP1/+ suppresses the rough-eye phenotype and increases the number of normal photoreceptors per ommatidium in flies expressing tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU.

Mekk1^EY02276/+ enhances the rough-eye phenotype and reduces the number of normal photoreceptors per ommatidium in flies expressing tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU. Increased incidence of ommatidium fusions are observed in these animals.

PP2A-B'^A131/+ enhances the rough-eye phenotype and reduces the number of normal photoreceptors per ommatidium in flies expressing tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU. Increased incidence of ommatidium fusions are observed in these animals.

Dl⁰⁵¹⁵¹/+ enhances the rough-eye phenotype and reduces the number of normal photoreceptors per ommatidium in flies expressing tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU. Increased incidence of ommatidium fusions are observed in these animals.

Expression of tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav.PU results in increased levels of photoreceptor cell division in third instar larval eye discs, which is suppressed by stg^EY12388/+.

(Rimkus et al., 2008)

Xenogenetic Interactions

Statement

Reference

BacA\p35^GMR.PH suppresses the rough eye and neurodegeneration phenotypes observed in flies expressing tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^GMR.PU.

Expression of BacA\p35^GMR.PH in third instar larval eye discs expressing tefu^{dsRNA.Scer\UAS} under the control of Scer\GAL4^elav.PU does not suppress the increased levels of cell division observed when tefu^{dsRNA.Scer\UAS} is expressed alone.

(Rimkus et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

tefu^RNAi.UAS

tefu^{dsRNA.Scer\UAS}

tefu^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

Open Close