Cuticle melanisation induced by B.bassiana is partially suppressed by expression of Spn77BaScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4Act.
Expression of Spn77BaScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4hs.PB at 18[o]C allows some Spn77BaW7 animals to grow to the second instar stage. These animals have melanised trachea and in most cases die before reaching the third instar stage.
Expression of Spn77BaScer\UAS.P\T.T:Ivir\HA1 under the control of Scer\GAL4hs.PB at 22-25[o]C allows some Spn77BaW7 animals to survive to the third larval instar or pupal stages. These larvae and pupae have melanised trachea.